Особливості клітинного циклу гепатоцитів при експериментальній неалкогольній жировій хворобі печінки та її корекції

Abstract

У статті розглянуті особливості показників клітинного циклу клітин печінки щурів, яким моделювали НАЖХП: кількість клітин у синтетичний період клітинного циклу (фазу S) більша порівняно з показниками у інтактних тварин, виявлено збільшення фрагментації ДНК та проліферативної активності клітин печінки. Застосування Ангіоліну зменшує проліферативну активність, активізує захисні та репаративні процеси, сприяє захисту клітин печінки від проапоптичного впливу стеатогепатиту; В статье рассмотрены особенности показателей клеточного цикла клеток печени крыс, которым моделировали НАЖБП: количество клеток в синтетический период клеточного цикла (фазу S) больше по сравнению с показателями у интактных животных, выявлено увеличение фрагментации ДНК и пролиферативной активности клеток печени. Применение Ангиолина уменьшает пролиферативную активность, активизирует защитные и репаративные процессы, способствует защите клеток печени от проапоптического влияния стеатогепатита; There remains an urgent problem of existence of pathological conditions (including liver disease), in which cells are characterized by increased susceptibility to signals that induce apoptosis. The purpose of the study – assess the impact of biologically active substance «Angiolin» with endothelial protective action on the cellular mechanisms of regeneration of liver tissue in NAFLD model. Materials and methods. Experimental studies were conducted on 30 adult white male rats. 20 rats were created a model of liver steatosis, some of the animals (10 rats) were treated with Angiolin administration (50 mg per kg of body weight). Maintenance and manipulation of the animals were carried out according to the «General ethical rules of experimentation on animals» approved by the first National Congress of Bioethics (Kyiv, 2001). The researchers were guided by the recommendations of the «European Convention for the Protection of vertebrate animals used for experimental and other scientific purposes» (Strasbourg, 1986) and the provisions of the «Good Laboratory Practice». The content of DNA in the nuclei of rat liver cells was determined by flow cytometry. Suspensions nucleus of liver cells were obtained using a special solution for the study of nuclear DNA CyStain DNA Step 1 from Partec, Germany, which allows you to quickly and simultaneously perform the extraction of cores and to mark nuclear DNA by 4,6-diamidino-2-phenylindole (DAPI). Results and discussion. The most significant differences between the parameters of the cell cycle of rat liver with created model of hepatic steatosis in the range SUB-G0G1 and relation to proliferation index was recorded. The signs of induction of apoptosis (DNA fragmentation more than 2.7 times), impaired DNA synthesis and increased proliferative activity of liver cells (6.2%) were found. The cell cycle of liver cells of rats with NAFLD model has its own characteristics: the number of cells in a synthetic cell cycle period (phase S) was higher in comparison with those in intact animals. The increase of DNA fragmentation and proliferation activity of liver cells was found. Using of Angiolin reduces proliferative activity, activates protective and reparative processes, help to protect liver cells fromи proapoptotic influence of steatohepatitis. Conclusions. Thus, we have proved that in the group of rats with the NAFLD model and treatment with Angiolin, liver cells was the most stable and activation of protective and reparative processes were marked.