Дія комплексного препарату Цитофлавін на біоелектричну активність еферентних волокон сідничного нерву в умовах експериментальної менопаузи

Abstract

Мета – вивчення змін активності еферентних нервових волокон під впливом препарату Цитофлавін в умовах менопаузи. Препарат вводився інтраперитонеально самицям білого щура через 120 днів після оваріогістероектомії протягом 10 днів. Аналізували збудливість та амплітудно-часові параметри викликаних відповідей нервових волокон. Зафіксовано зменшення хронаксії на 8,85±1,46% (р<0,01), латентного періоду та загальної тривалості відповіді на 23,19±2,64% (р<0,01) та 29,48±3,39% (р<0,01) відповідно. При нанесенні парних подразнень спостерігалося більш раннє відновлення амплітуди відповіді на другий стимул на інтервалах 3-5 мс.; Цель – изучение изменения активности эфферентных нервных волокон под влиянием препарата Цитофлавин в условиях менопаузы. Препарат вводился интраперитонеально самкам белой крысы через 120 дней после овариогистероектомии в течение 10 дней. Анализировали возбудимость и амплитудно-временные параметры вызванных ответов. Зафиксировано уменьшение хронаксии на 8,85±1,46%, латентного периода на 23,19±2,64% и общей продолжительности ответа на 29,48±3,39% (р<0,01). При нанесении парных раздражений наблюдалось более раннее восстановление амплитуды второго ответа на интервалах 3-5 мс.; In our previous studies, it was found that the conditions of the early emergence and continued existence of estrogen deficiency is an increase in the excitability of efferent fibers of the sciatic nerve against the background of increasing chronaxie, latency and reduced lability. These changes can be explained by hypoxia neural structures against the background of increased sensitivity to lack of oxygen caused by estrogen deficiency to their neuroprotective properties, violation of myelination processes, decreased activity of Na + / K + -ATPase. The aim of this study was to investigate the influence of a combination of succinic acid riboksin, riboflavin and nicotinamide, in the preparation Cytoflavin that has antioxidant and metabolic properties on bioelectrical activity of efferent nerve fibers under experimental menopause. The rationale for the use of the drug Cytoflavin is that its components incentives respiration and energy production in cells, improves utilization of oxygen and glucose in the tissues, reducing the activity of antioxidant enzymes, activating intracellular protein synthesis, help resynthesis γ-aminobutyric acid neurons. Experiments were conducted on 26 white mature female rats Wistar weighing 200- 250 g, 120 days after total ovariohisteroektomia to create experimental menopause. Rats of experimental group received intraperitoneally Cytoflavin drug solution diluted 10 times physiological sodium chloride 0.9% 0.13 ml / 100 g weight 1 per day for 10 days. The animals in the control group performed intraperitoneally injected with sodium chloride 0.9%. The diversion caused by the activity carried on proximal ventral root with stimulation of the sciatic nerve pulse duration of 0.3 ms and strength from 1 to 5 rapids (P). Analyzed the following parameters: threshold, chronaxie, latent period, amplitude and duration of the action potential. Also investigated the phenomenon refractory by applying paired stimuli with intervals of 2 to 20 ms. Processing of the data was performed using standard methods of variation statistics. The reliability of the data was assessed using parametric methods (Student test) and nonparametric statistics (Mann-Whitney criterion). There was a decrease in chronaxie 8,85±1,46% (p<0.01) latency to 23,19±2,64% (p<0.01) and the total duration answers to 29,48±3,39% (p<0.01). When applied to paired stimuli observed earlier recovery of the amplitude of second response for interstimulus intervals from 3 to 5 ms. Improved time characteristics induced responses may explain the subsequent action of the drug components. Riboflavin provides maintenance and redox reactions controlled flavin coenzyme. It is a coenzyme glutathione reductase restoring pool of glutathione – a main component of antioxidant system cells. Riboflavin is also part of the mitochondrial respiratory enzymes that are able to ensure the regeneration of NAD +. Nicotinamide is a precursor nucleotides dehydrogenase (NAD + and NADP +). Value NADH / NAD + is a major regulatory mechanism for tricarbonic acid cycle and partially – oxidative phosphorylation. Action succinic acid under hypoxic conditions implemented in tricarbonic acid cycle and oxidative phosphorylation. It accelerates the turnover succinate – fumarate – malate and reduces the concentration of lactate. The consequence of increasing the circulation of tricarbonic acid cycle is to increase the amount of energy required for the synthesis of ATP and GABA. Succinic acid increases the oxygen consumption of tissues and improves tissue respiration by increasing electron transport in the mitochondria, play proton gradient in their membranes and the shift of oxyhemoglobin dissociation curve to the right, and thus increases the returns oxygen to the tissues. Positive changes in energy supply subcellular structures under the influence of antioxidant and antihypoxic effect of Cytoflavin can lead to increased lability of nerve fibers through the improvement of 3NA + / 2K + ATPase, as observed in experimental animals of this study.