Вплив метаболітів стрептоміцетів на фізіологічну активність кукурудзи

Abstract

Мета. Дослідити умови приживаності стрептоміцету штаму Streptomyces recifensis var. lyticus П-29 в ризосфері кукурудзи сорту Аквазор. Встановити вплив стрептоміцету на морфобіохімічні ознаки рослини. Методи. Використовували біохімічні, біометричні, мікробіологічні методи. Результати. Встановлено, що максимальний показник приживаності стрептоміцету штаму S recifensis var. lyticus П-29 у ризосфері кукурудзи сорту Аквазор складав 40,2%, що супроводжувалося збільшенням ваги рослини на 18,0% та підвищенням пероксидазної активності коренів на 15,0%. Результати дослідження можуть бути використані у сільському господарстві для виготовлення високоефективних препаратів, які проявляють рістстимулюючу дію до рослин; Цель. Исследовать условия приживаемости стрептомицета штамма Streptomyces recifensis var. lyticus П-29 в ризосфере кукурузы сорта Аквазор. Установить влияние стрептомицета на морфобиохимические признаки растения. Методы. Использовали биохимические, биометрические, микробиологические методы. Результаты. Установлено, что максимальный показатель приживаемости стрептомицета штамма S. recifensis var. lyticus П-29 в ризосфере кукурузы сорта Аквазор составлял 40,2%, что сопровождалось увеличением веса растения на 18,0% и повышением пероксидазной активности корней на 15,0%. Результаты исследования могут быть использованы в сельском хозяйстве для производства высокоэффективных препаратов, которые проявляют ростстимулирующее действие на растения; To improve the growth and development of plants with the aim of increasing yields in agriculture apply plant growth stimulants and physiologically active substances. These compounds accelerate the germination of seeds, root development, flowering, fruiting, governing the rest of the plants and ensure their resistance to disease. Such biologically active substances are capable of synthesizing microorganisms based on active substances which are made of biological products. Active strains of microorganisms (which are components of the biological products) do not cause human genetic effects like chemical remedies. Replacement of hazardous chemicals biological will reduce the use of fertilizers and pesticides in agriculture that will produce clean, high quality crop products. Therefore, an important issue today remains the creation and application of biological preparations on the basis of active strains of microorganisms and their metabolites for increasing the yield of cultivated plants. The aim of this work was to investigate the conditions of survival streptomycete strain Streptomyces recifensis var. lyticus P-29 in the rhizosphere of maize varieties Equator. To determine the influence streptomycete on Morton signs of plants. The object of the study were: the stimulating effect monoporosa suspension of the strain Streptomyces recifensis var. lyticus P-29 corn varieties Equator. A study of the stimulating action of metabolites streptomycete on corn varieties Equator was performed using monoporosa suspension of strain S. recifensis var. lyticus P-29. Controlled the dynamics of the development streptomycete in the sand (K1), the development of plants in sand without streptomycete (K2) and the development of plants, as spores streptomycete in germinated corn grain. The results of research and their discussion. The survival rate streptomycetes in the rhizosphere of maize discovered in the maximum number of 40.2% on the 12th day from the start of the experiment. However, in the future, the survival rate of spores gradually decreased from 8.7% to 6.5%. In the experiment, in addition to survival streptomycete studied changes mortoni signs. If you make spores of the strain P-29 under the corn germ in the amount of 14.1 ± 0,03) • 107 CFU/ml was observed an increase in the root length average of 33% throughout the observation with the maximum length on the 19th day of the experiment. With the length of the roots increased their weight, the value of which increased by an average of 26%. Also supervised the changes that have occurred in the ground part of the plant. Thus, over the period of the experiment, the length of the coleoptile in seedlings increased by 22% and weight by 32%. In General, observed increase of weight of plants 64%. It should be noted that with the increase of biometric parameters of the plants were observed changes in enzymatic activity. So, the peroxidase activity of roots was increased because of the presence of spores in the rhizosphere streptomycete 10-15% in comparison with the control.