Морфологічна характеристика формування капсули суглобу щурів у післянатальному періоді у нормі та експерименті

Abstract

На тлі вивчення формування капсули колінного суглоба щурів в післянатальному періоді в нормі та після моделювання змін в системі мати-плацента-плід встановлено, що перехідна частина капсули суглоба є ключовою структурою, що забезпечує підтримку цілісності суглоба як органу, оскільки в цій області визначається контакт між різними структурними компонентами суглоба: волокнами капсули, клітинами синовіального шару, матриксом хряща і безпосередньо хондроцитами суглобового хряща. Порушення її цілісності, на нашу думку, може спричинити зміну морфофункціональних властивостей як синовіального шару капсули, так і суглобового хряща; На фоне изучения формирования капсулы коленного сустава крыс в постнатальном периоде в норме и после моделирования изменений в системе мать-плацента-плод установлено, что переходная часть капсулы сустава является ключевой структурой, обеспечивающей поддержку целостности сустава как органа, поскольку в этой области определяется контакт между различными структурными компонентами сустава: волокнами капсулы, клетками синовиального слоя, матриксом хряща и непосредственно хондроцитами суставного хряща. Нарушения ее целостности, по нашему мнению, может привести к изменению морфофункциональных свойств как синовиального слоя капсулы, так и суставного хряща; The article is devoted to the problem of joint capsule development and reactivity. The purpose of the article is to give morphological characteristic to the process of joint capsule development in normal conditions and after changes in the system of «mother-placenta-fetus». Оbject and methods. Knee joint capsules of five groups of rats were investigated in the work since birth up to the 12 day of postnatal life. The first group consisted of intact rats. The second one was undergone intrafetal antigen injection at day 17 of gestation. The third group was a control one in comparison with the second group. Pregnant rats of the fourth group got hydrocortisone injections throughout the third period of pregnancy. The fifth group was a control for the fourth one. Joint capsule was examined at 1-st, 7-th, 11-th, 14-th, 21-st, 30-th, 45-th, 60-th, 90-th and 120-th day after birth. Hystological samples were stained with hematoxilin-eosin, alcyan blue. Lectin-hystochemic asseys were also conducted. All quantities data were statistically estimated (p<0,05). Results and discussion. On the basis of the conducted complex of research methods (macro-microscopic, histological, histochemical, lectinhistochemical and immunohistichemical) the features of morphogenesis of rats’ knee-joint from a moment of birth up to 120-th day of life and peculiarities of reactivity of rats’ knee-joint after an antigen and hormonal influence in the antenatal period of development were analysed. The leading role of transitional part and lymphocytes in maintance of joint integrity as organ and its role in realization of reactivity of joint as an organ after antigen and hormonal intranatal influence was approved. A common synovial layer is distinguished. It covers the cavity of joint and form an innate immunological barrier, impedimental to destruction of cartilage from the side of synovia after influence of factors of antigen and hormonal nature. In rats after intranatal antigen injection the reliable increase of maintenance of PNA+-lymphocytes is determined. On the background of enhanceable maintenance of PNA+-lymphocytes in transitional zone more earlier formation of subchondral bone is determined. In rats, got from females after introduction of hydrocortione during pregnancy general amount of lymphocytes, PNA+-lymphocytes diminishes as compared to control. That is accompanied by more earlier as compared to control thinning of articular cartilage, diminishing of relative area is determined by occupied by fibres their disorganization, shortening and diminishing of depth of penetration in the matrix of articular cartilage of the joined bones, is determined more later as compared to control appearance of elastic fibres.