Динаміка змін показників перокисного окислення та антиоксидантної системи при лікуванні генералізованого пародонтиту початкового ступеня розвитку у осіб, що проживають на екологічно-забруднених територіях Прикарпаття

Abstract

Місцеве застосування гель кварцетину який активували за допомогою лазерного опромінення хворим на генералізований пародонтит 1-го та ІІ-го ступеня сприяло покращенню показників про- та антиоксидантної системи в сироватці крові (ТБК-активних продуктів, активності ферментів глутатіонпероксидази та супероксиддисмутази).

Местное применение гель кварцетина который активировали с помощью лазерного облучения больным на генерализованный пародонтит I-й и II-й степени способствовало улучшению показателей про- и антиоксидантной системы в сыворотке крови (ТБК-активных продуктов, активности ферментов глутати- онпероксидазы и супероксиддисмутазы).

Local application of gel quercetin which is activated using laser irradiation to patients with generalized periodontitis of I and II degree, contributed to the improvement of the indicators of pro- and antioxidant system in blood serum (TBA-active products, the activity of the enzymes glutathione peroxidase and superoxide dismutase). According to the obtained data the concentration of TBA-active products is significantly increased in patients with GP before treatment, that means an increase in oxidating stress in patients. However, there is an increase in the activity of ceruloplasmin, glutathione peroxidase and decrease in SOD activity, indicating the exhaustion of the AOS, indexes are true. Complex treatment to patients with GP led to an improvement of pro-as well as antioxidant system when using quercetin (IA, IB group), and when using quercetin in combination with laser therapy (IIA and IIB group). In group IIA compared with group IA, after three months of treatment, there is a significant decrease in the number of TBA-active products by 5.47% (p < 0.05), after 6 months - by 1.26% (p < 0.05) and after 12 months - by 3.1 % (p < 0.01). And in patients with GP of II degree (IIB-IB) there is a decrease in the number of TBA-active products - by 2.40% (p < 0.05) after three months, after 6 months - by 1.42% (p < 0,05), after12 months - by 1.16% (p < 0.01). The results indicate that patients with GP of I degree managed to achieve better results after the treatment than patients with GP of II degree, due to a lighter course of the disease; it indicates the reduction of lipid peroxidation and stabilization of cell membranes. As for ceruloplasmin, a decrease in the level of ceruloplasmin has been observedin patients with GP of I degree (IA-IIA) after three months by 1.52% (p < 0.01), after 6 months - by 2.86% (p < 0.001), after 12 months - by 2.41% (p < 0.001). And there was a decrease in the level of ceruloplasmin in patients with GP of II degree (IB-IIB) after three months by 1.41% (p < 0.01), after 6 months - by1.70% (p< 0.001) and after 12 months - by 1.29% (p < 0.001). So after three months of treatment we have achieved the same improvementin both groups, indicating the reduction of the inflammatory process. But a significant improvement was observed in patients with GP of I degree after 6 and 12 months. A significant improvement of results has been observed in patients with GP of I-degree (IA-IIA) after three months - the levels of glutathione peroxidase decreased by 7.48% (p < 0.05), after 6 months it dropped by 12.83% (p< 0.01), and after 12 months - by 9.5% (p< 0.05). However, a steady improvement of results has been observed in patients with GP of II degree (IB-IIB) throughout the year. After three months there was a decrease by 11.16% (p < 0.01), after 6 months by 11.57% (p< 0.001) and after 12 months by 11.11% (p< 0.01). So we managed to stabilize the level of glutathione peroxidase, which also indicates the reduction of lipid peroxidation. The increase in the concentration of SOD by 2.71% (p < 0.05) after three months, by 2.07% (p< 0.01) after 6 months and by 2.84% (p< 0.001) after 12 months has been observed in patients with GP of I-degree (IA-IIA). The SOD concentration in patients with GP of II degree (IB-IIB) has been increased by 1.76% (p < 0.05) after three months, after 6 months it increased by 1.63% (p< 0.05) and after 12 months - by 1.9% (p< 0.001). So, as in the case of TBA-active products the situation has become much better for patients with GP of I-degree. So, after the complex treatment of patients with GP of both groups by using the proposed methods, there is a decrease of the activity of both antioxidant system indexes - ceruloplasmin and glutathione peroxidase, and a decrease in the concentration of the finished TBA-active products of LPO in the setting of the growth of SOD. In result of the conducted researches it has been established that the use of laser therapy along with quercetinum stimulates metabolic processes in periodontal tissues and provides reliable improvement of clinical indicators of the treatment efficiency and can be proposed for the treatment of patients with GP of I-II degree of severity, living in ecologically contaminated areas.