Возрастные особенности роста и формообразования костей скелета после 60-дневного воздействия на организм паров эпихлоргидрина

Abstract

60-денний вплив парів епіхлоргідріна з експозицією 5 годин в 10 ГДК супроводжується гальмуванням темпів росту великогомілкової і кульшової кісток, а також третього поперекового хребця, виразність і темпи відновлення якого залежать від віку піддослідних тварин. Найшвидше темпи росту досліджуваних кісток відновлювалися у статевонезрілих щурів, в період інволютивних змін відновлення було мінімальним. Застосування на тлі інгаляцій епіхлоргідріна тіотриазоліна в дозуванні 117,4 мг/кг маси або настоянки ехінацеї пурпурової з розрахунку 0,1 мг сухої речовини на 100 г маси супроводжувалося згладжуванням виявлених змін. Використання тіотриазоліну було більш ефективним, ніж застосування ехінацеї.

60-дневное воздействие паров эпихлоргидрина с экспозицией 5 часов в 10 ПДК сопровождается торможением темпов роста большеберцовой и тазовой кости, а также третьего поясничного позвонка, выраженность и темпы восстановления которого зависят от возраста подопытных животных. Быстрее всего темпы роста исследуемых костей восстанавливались у неполовозрелых крыс, в период инволютивных изменений восстановление было минимальными. Применение на фоне ингаляций эпихлоргидрина тиотриазолина в дозировке 117,4 мг/кг массы либо настойки эхинацеи пурпурной из расчёта 0,1 мг сухого вещества на 100 г массы сопровождалось сглаживанием выявленных изменений. Использование тиотриазолина было более эффективным, чем применение эхинацеи.

The aim of the present study was to examine growth of the tibia and the hipbone, as well as the third lumbar vertebra, in rats after 60 day inhalation of epichlorihydrin and to find ways of correction of its negative effects with thiotriazoline and Echinaceae Tinctura. Object and methods. The experiment involved 420 male rats of three age groups (young, mature and old). Each age group was further separated into the following groups: intact animals, animals that received daily epchlorohydrin inhalations as a single 5-hour exposure to 10 MPC for 60 days and the groups 3 and 4 received 2.5% intraperitoneal solution of thiotriazoline in dosage 117.4 mg per kg of body weight and per os Echinaceae Tinctura in dosage of 0.1 mg of active component per 100 g of body weight. The animals were withdrawn from the experiment by the 1st, 7th, 15th, 30th and 60th days after discontinue of 60 day cycle of epichlorohydrin inhalations. The tibiae, hipbones and third lumbar vertebrae were excised and put to gross measurements (V.I. Luzin, 2004). The data obtained were analyzed by means of variation statistics using standard applied software. Results and discussion. By the first day after epichlorohydrin discontinue, the maximum length of the tibia and hipbone and the height of the body of the third lumbar vertebra in young animals decreased as compared to the control values by 9.26%, 7.45% and 8.33%. In adult animals the same values changed in the same way by 6.09%, 6.32% and 4.86% and in old animals – by 4.72%, 5.45% and 4.16%. In readaptation period young animals exhibited restoration of deranged features by the 30th day of observation, in adult animals alterations persisted up to the 30th day of observation and old animals did not exhibit marked restoration. Administration of thiotriazoline or Echinaceae Tinctura reduced negative effects of epichlorohydrin during inhalation and after it. After thiotriazoline administration, deranged features of the growth rare of skeletal bones restored in young animals by the 1th to 15th days of observation, in adult animals exhibited by the 15th to 60th days of observation and in old animals signs of restoration were observed by the 30th and the 60th days. After Echinaceae Tinctura administration, in young animals restoration of the growth rare of skeletal bones was observed by the 1th to 15th days of observation and in adult and old animals – by the 30th and the 60th days of observation. Generally thiotriazoline appeared to be more effective than Echinaceae Tinctura. Conclusions: 60-days of the epychlorhidrine inhalations in dosage of 10 MPC as a single 5-hour exposure per day caused the deceleration of growth of the tibia and the hipbone, as well as the third lumbar vertebra, with the age-dependent mode. Reparation of the growth rate of bones was possible in young organisms, but in older age groups changes have become unimproved. Administration of the thiotriazoline in dosage of 117.4 mg per kg of body weight or tincturа of Echinacea Purpurеa in dosage of 0.1 mg of active substance per 100 grams of body weight prevents the deceleration of growth of skeletal bones after the 60-days influence of the epychlorhidrine vapors, but the thiotriazoline has much more reparative potency.