Analysis of specific groups of microorganisms isolated from atherosclerotic coronary arteries of patients depending on Asp299Giy polymorphism of TLR4 gene.

Abstract

The aim of this work was to establish the presence of specific groups of microorganisms, including paradontopathogenic in atherosclerotic plaque and surrounding tissues and the dependence of microbial contamination of polymorphism 896A / G gene TLR4 (rs4986790), and to identify the possible relationship of atherosclerosis and Asp299Giy gene polymorphism TLR4 in patients with coronary heart disease (CHD). Materials and methods: The 31 autopsies of coronary arteries from patients whodied from coronary arteries disease and 5 from healthy individuals. Results and discussions: The presence of periodontal pathogenic microorganisms in atherosclerotic plaques was observed in 83,9%. In 51,6% two or more species of microorganisms were found. Only in 11,1%> samples with atherosclerotic plaques the microorganisms were found in undamaged tissues. The patients who died from coronary arteries disease had 299Giy allele of TLR4 gene more frequent than healthy individuals (p=0,04) OR 2,92 (1,15-7,41). The presence of TLR4 gene polymorphic allele G in the individual genotype determines the increased contamination of atherosclerotic plaque tissues by the representatives of the following genera: Lactobacillus sp, Enterobacterium sp, Sneathia sp. /Leptotrihia sp. /Fusobacterium sp, Mobiiuncus sp. /Corynebacterium sp., Peptostreptococcus sp. The emergence of new correlation pairs with participation of Lachnobacterium sp. /Corynebacterium sp. among the carriers of G allele has been revealed via the intragroup correiation analysis. Conclusion: The obtained results confirm the possible involvement of the represented groups of microorganisms in the pathogenesis of atherosclerosis and the role of the TLR4 gene polymorphic variant G in the increased microbial contamination of the coronary arteries tissues. The presence of299Giy allele of TLR4 gene increases the risk of this disease.