Активність орнітиндекарбоксилази та α-амілази у тканинах слинних залоз щурів за умов глутамат-індукованого ожиріння

Abstract

Метою дослідження було вивчення активності орнітиндекарбоксилази та α-амілази у тканинах слинних залоз щурів за умов глутамат-індукованого ожиріння. За умов моделювання глутамат-індукованого ожиріння виникають патологічні зміни у тканинах слинних залоз щурів: зменшення активності орнітиндекарбоксилази та α-амілази, що свідчить про пригнічення білоксинтезуючої функції.; Целью исследования было изучение активности орнитиндекарбоксилази и α-амілази в тканях слюнных желез крыс при условиях глутамат-индуктируемого ожирение. В условиях моделирования глутамат-индуцированного ожирения возникают патологические изменения в тканях слюнных желез крыс: уменьшение активности орнитиндекарбоксилазы и α-амилазы, что свидетельствует об угнетении белоксинтезирующей функции.; Obesity is currently regarded as pandemic. According to the data of World Health Organization there are more than a billion overweight people on the planet. According to the epidemiological studies conducted in Ukraine more than half of the adult population is overweight. Low levels of daily physical activity combined with intake of food with excess calories, as well as extensive and uncontrolled use in food industry of food additives, primarily of monosodium glutamate, favor an increase in adiposity. According to the literature, obesity and pathologies associated with it can lead to decreased functioning of cells of salivary glands, reduced saliva flow, increased viscosity of saliva, development of xerostomia. Impaired functioning of salivary glands leads to the development of pathological processes in the oral cavity, increases danger of tooth decay and periodontal disease development, causes a disturbance of the digestive processes in other parts of the gastrointestinal tract. However, pathological mechanisms involved in the impact of obesity on salivary glands are not fully established. The aim of the study was to determine the activity of ornithine decarboxylase and α-amylase in the tissues of rats’ salivary glands under monosodium glutamate-induced obesity. The study was done on 20 rats. We modeled monosodium glutamate-induced- obesity in rats. The rats were divided on 2 groups at the beginning of the experiment. I group was intact control. Newborn rats of II group were administered monosodium glutamate (4 mg/g) at 2, 4, 6, 8, 10 days of life. After 4 months of experiment animals were determined body mass index (BMI). In the homogenate of rats’ salivary glands we determined the activity of ornithine decarboxylase and α-amylase. It was established that BMI of rats of II group significantly increased in 1.21 times compared with the control group. Thus, the rats of II group were observed a significant increase of BMI compared with control group that indicates on the development of obesity. It was found that there is credible decrease of activity of ornithine decarboxylase in 1.30 times in the tissues of salivary glands of rats under monosodium glutamate-induced obesity.