Please use this identifier to cite or link to this item: http://repository.pdmu.edu.ua/handle/123456789/17692
Title: Alterations of the intensity of neun-immunoreactivity reactions in the cerebellar structural components of rats under influence of the food additives complex
Authors: Bilash, S.
Kononov, B.
Pronina, O.
Koptev, M.
Hryn, V.
Білаш, Сергій Михайлович
Кононов, Богдан Сергійович
Проніна, Олена Миколаївна
Коптев, Михайло Миколайович
Гринь, Володимир Григорович
Issue Date: Jan-2022
Publisher: Georgian medical news
Citation: Alterations of the intensity of neun-immunoreactivity reactions in the cerebellar structural components of rats under influence of the food additives complex / S. Bilash, B. Kononov, O. Pronina, M. Koptev, V. Hryn // Georgian medical news. – 2022. – № 1 (322). – P. 145–149.
Abstract: To estimate the changes of the intensity of the NeuN-immunoreactivity reaction in the structural components of the cerebellum of rats under the influence of the food additives complex. We used 100 white rats with an average weight of 258.1±0.67 g. in our study. We used paraffin sections with a thickness of 3 μm for immunohistochemical studies. Rat monoclonal antibody NeuN (0.N.211) sc-71667 (Santa Cruz Biotechnology, inc.) was used to detect neurons. During the morphometric study we identified that in the course of the experiment, the average number of NeuN-positive Purkinje cells of the ganglion layer of the cerebellar cortex of rats in different experimental groups (from 1st to 5th ) in comparison with the control group of animals significantly increases at p˂0.05. Concurrently, we determined morphometrically that in the course of the experiment the average number of NeuN - positive Golgi cells of type II of the granular layer of the cerebellar cortex of rats in different experimental groups in comparison with the control group of animals also increased significantly at p˂0.05. In our experimental study, we determined reliably that NeuN- positive structures of the gray matter of the cerebellum were different, both structurally and morphometrically.
Keywords: NeuN
rat
cerebellum
NeuN-immunoreactivity reaction
cells
ISSN: 1512-0112
URI: http://repository.pdmu.edu.ua/handle/123456789/17692
Appears in Collections:Наукові праці
Наукові праці

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