Лектинова активність вуглеводних детермінант структурних компонентів воротарного відділу шлунку при гострому експериментальному гастриті та введенні кріоконсервованої плаценти

Abstract

Для оцінки функціонального стану поверхнево-ямкового епітелію самою доказовою визначилась реакція з лектином PNA. Зменшення частоти зв’язування в п’ятій експериментальній групі свідчить про зменшення синтезу та секреції шлункового соку,що посилює альтеративні процеси. В шостій та сьомій експериментальних групах реакція з цим лектином збільшується, що є підтвердженням посилення компенсаторно-захисних механізмів під дією препарату «Платекс-плацентарний». Звязування з лектином SBA визначається вибірково тільки для кислотних екзокриноцитів, що дозволяє рекомендувати його у якості селективного маркеру для визначення морфофункціонального складу пристінкових гландулоцитів; Для оценки функционального состояния поверхностно-ямочного эпителия самым доказательным определилась реакция с лектином PNA. Уменьшение частоты связывания в пятой экспериментальной группе свидетельствует об уменьшении синтеза и секреции желудочного сока, что усиливает альтеративные процессы. В шестой и седьмой экспериментальной группе реакция с данным лектином усиливалась, что есть подтверждением усиления компенсаторно-защитных механизмов под воздействием препарата «Платекс-плацентарный». Связывание с лектином SBA определяется выборочно только для кислотных экзокриноцитов, что позволяет рекомендовать его в качестве селективного маркера для определения морфофункционального состава пристеночных гландулоцитов; Abstract. The purpose of the study was to identify changes of carbohydrate specificity of cellular surfaces of structural elements of pyloric part of stomach in administration of cryopreserved placenta in connection with experimental acute gastritis. The object of experimental study were forestomach gastrobiobsy materials from 175 Wistar senior male rats. The animals were divided into seven groups: 10 intact animals were assigned to group I; 10 animals, administered intraperitoneally with 1 ml of saline were assigned to control group II; 10 animals who were made incision on the outer surface of the hip were assigned to control group III; 10 animals, administered intraperitoneally with 1 ml of saline and with incision on the outer surface of the hip were assigned to control group IV; 45 animals with simulated acute gastritis, induced by intraperitoneal administration of 5 mg of l-carrageenan in 1ml of saline per one animal were assigned to experimental group V; 45 animals, administered with “Platex-platsentarnuy” medication one time were assigned to experimental group VI; 45 animals, administered subcutaneously with “Platex-platsentarnuy” medication one time in connection with simulated acute gastritis were assigned to experimental group VII. Study of glucoconjugates of Sambúcus nígra bark (SNA) lectin, specific to sialic acid, established strong relationships with receptors of apical membrane of stratified squamous epithelial cells, granules of cervical mucous cells and pyloric exocrinocytes of glands’ bottom in intact rats. The outcome of SNA lectin conjugations with granules of pyloric exocrinocytes of glands’ body of pyloric part of stomach was moderate in intact rats. In the fifth experimental group of animals the reaction of luminal surface of stratified squamous epithelium decreased by 50 %. Granules of cervical mucous cells showed negative reaction. Unlike rats from intake group, the surface of main exocrinocytes of glands’ body showed moderate expression, and the surface of main exocrinocytes of glands’ bottom showed strong expression. Relationship of granules of main exocrinocytes of glands’ bottom to SNA lectin increased by 25 %. SNA-positive conjugates on the stratified squamous epithelial cells and granules of the main exocrinocytes of glands’ body decreased by 25 % in the sixth experimental group of animals. Occurrence of poor SNA-marking has been detected on the surface of pyloric exocrinocytes of glands’ body. It has been revealed that SNA-apical membrane of stratified squamous epithelium and granules of cervical mucous cells conjugation decreased by 50 % in the animals from the seventh experimental group, as compared to intact animals; by 25 % – with granules of pyloric exocrinocytes of stomach pyloric glands’ body. However, poor conjugation has been defined for the surface of pyloric exocrinocytes of glands’ body and moderate for the surface of exocrinocytes of glands’ bottom. Lectinohistochemical study provided with detailed description of morphofunctional changes in cardiac, fundic and pyloric parts of stomach wall of rats in experiment. Changes of expression of receptors of sialospecific lectins are the markers of impairment of protective function of stratified squamous epithelial cells’ mucus and intensification of proliferation processes. Galactosospecific lectins promote estimation of secretion production, maturing of secretory granules and their excretion into glands’ lumens. They can also provide with assessment of mitotic activity of epithelial cells. Fucoso- and mannosospecific markers can be used to estimate the quality of mucus secretion by epithelial cells of stomach. Conclusion. Lectinohistochemical study showed detailed morphofunctional changes in pyloric part of rat stomach wall in experiment. Changes of expression of receptors of sialospecific lectins are the markers of impairment of protective function of stratified squamous epithelial cells’ mucus and intensification of proliferation processes. Galactosospecific lectins promote estimation of secretion production, maturing of secretory granules and their excretion into glands’ lumens. They can also provide with assessment of mitotic activity of epithelial cells. Fucoso- and mannosospecific markers can be used to estimate the quality of mucus secretion by epithelial cells of stomach.