Реакція гемомікроциркуляторного русла наднирника при корекції гострого асептичного перитоніту введенням кріоконсервованої плаценти у щурів

Abstract

Надниркові залози, як важливі складові нейроендокринної та стрес-адаптаційної систем, відповідають за реалізацію та мобілізацію захисних реакцій організму під час дії на нього різноманітних патогенних чинників. Гемомікроциркуляторне русло наднирників є основною ланкою, яка реагує на них зміною діаметру його елементів, зміною структури стінки, що поєднується з лейкоцитарною інфільтрацією периваскулярної сполучної тканини; Надпочечники, как важные элементы нейроэндокринной и стресс-адаптационной систем, отвечают за реализацию и мобилизацию защитных реакций организма во время действия на него различных патогенных факторов. Гемомикроциркуляторное русло надпочечников является основным звеном, которое реагирует на них изменением диаметра его элементов, изменением самой стенки и сопровождается лейкоцитарной инфильтрацией периваскулярной соединительной ткани; Introduction. It is proved that the adrenal glands as important components of neuroendocrine and stress-adaptive systems, are responsible for the implementation and mobilization of defense reactions of organism during the influence of various pathogenic factors on it. Therefore, the study of the morphofunctional changes in adrenal tissue during aseptic peritonitis and its correction with transplantation of the cryopreserved placenta is relevant. The aim of the research was to study hemomiсrocircular channel (HMCC) of the cortex and medulla of the adrenal glands when administering cryopreserved placenta during aseptic peritonitis. Material and research methods. Work is performed on 95 white male rats of “Vistar” line, divided into 3 groups. Group I – 5 intact animals, group II – 45 animals which were infected with acute experimental aseptic peritonitis via administering intraperitoneally of 5 mg λ-carrageenen («Sigma», USA) in 1 ml of isotonic solution of NaCl per an animal, group III – 45 animals which at simulated experimental acute aseptic peritonitis were made one-time transplantation of cryopreserved placenta (CPP). The withdrawal of animals from the experiment was carried out by an overdose of thiopentonal anesthesia on 1st, 2nd, 3rd, 5th, 7th, 10th, 14th, 21st and 30th day. Research of adrenal tissue was carried out according to established deadlines. The tested material was compacted into paraffin and epoxy resin with conventional methods and was used to produce histological sections, which were stained with hematoxylin-eosin (paraffin sections) and methylene blue (half-thin sections). The internal diameter of cortex capillaries (glomerular, zona fasciculata, reticular zone) and of medulla was measured. We used a microscope “Micromed XS-5510” with digital photomicroattachment of «Micromed» company with program TSView adapted for such researches. Mathematical analysis of the material was performed using standard methods of variation statistics: calculation of the mean values (M), the error of the mean values (m), Student’s test (t). Differences were considered reliable at p <0.05. Results of the research and their discussion. Increasing of the diameter of capillaries of glomerular zone (CGZ) in the third group from 5th to 10th day, compared to the intact group and from 7th to 10th days, compared to the second group confirms the property of CPP to strengthen the implementation of inflammation of the glomerular zone from the 7th to 10th day of the experiment. The diameter of the capillaries of zona fasciculate (CFZ) of the third group relatively to the diameter of capillaries of the intact group grew at 1st, 5th and 7th day, while compared to the second group the increase of the size of CFZ did not happen, which indicates the ability of CPP to neutralize the impact of aseptic peritonitis on zona fasciculate. Conclusions. It is found that when transplanting CPP at aseptic peritonitis a significant increase of the mean values of HMCC of adrenal cortex and medulla, which are manifested by 7-10 day of the study, takes place.