Стан антиоксидантної системи легень при гострому емоційно-больовому стресі у щурів з різним типом реагування та його корекція глутапіроном

Abstract

В статті наведені результати впливу гострого стресу на легеневу тканину щурів з різним типом реагування. За цих умов спостерігається активація вільнорадикальних процесів на фоні зниження активності антиоксидантної системи, про що свідчить накопичення ТБК-реактантів, зменшення вмісту аскорбінової кислоти та зниження активності каталази. Показано, що вказані зміни більш виражені у щурів з активним типом реагування. Також наведено дані, що глутапірон ефективно нормалізує патологічні зміни у легеневій тканині щурів за умов гострого стресу; В статье представлены результаты влияния острого стресса на ткани легких крыс с разным типом реагирования. В этих условиях наблюдается активация свободно-радикальных процессов на фоне снижения активности антиоксидантной системы, о чем свидетельствует накопление ТБК-реактантов, уменьшение содержания аскорбиновой кислоты и снижение активности каталазы. Показано, что указанные изменения более выражены у крыс с активным типом реагирования. Также представлены данные, что глутапирон эффективно нормализует патологические изменения в легочной ткани крыс в условиях острого стресса; Lung injury is a frequent consequence of acute stress. Better understanding of the mechanisms of pathological changes at acute emotional-pain stress contributes to searching for effective methods of treatment. Objective. The aim of our research is a study of free-radical oxidation and changes of antioxidant system in rats lungs with different types of reaction under condition of acute emotional-pain stress and their correction by glutapyrone. Object and methods. Experiments were carried out on 78 male Wistar rats, weight 150-180 g. The rats were divided into three groups (active, middle, passive). It was used open field test. Experimental investigation was carried out in accordance with international bioethical principles and the laws of Ukraine. We modeled acute emotional-pain stress on the rats of experimental and control groups (model by O. Disederato). The intensity of the free-radical processes was evaluated on the basis of the content of malondialdehyde (MDA) and antioxidant system – based on the activity of catalase, the content of ascorbic acid in homogenates of the lungs. Glutapyrone was injected intraperitoneally (1 mg/kg) after modelling of acute emotional-pain stress. Results. Under conditions of emotional-pain acute stress is observed changes of antioxidant system in rats lungs. It was found the increasing of MDA in lungs: rats from active group – by 1,67 times (р<0,05), after correction by glutapyrone by 1,24 times (р<0,05); rats from passive group – by 1,42 times (р<0,05), after correction – by 1,21 times (р<0,05) compared to the control. This indicates the development of oxidative stress in rats under acute emotional-pain stress. Under these conditions it was observed the decrease of catalase and ascorbic acid in lungs. The activity of catalase decreased: in active group – by 1,85 times (р<0,05), after correction by 1,26 times (р<0,05); in passive group – by 1,53 times (р<0,05), after correction – by 1,17 times (р<0,05) compared to the control. It was observed the decreasing of ascorbic acid level in lungs: rats from active group – by 1,32 times (р<0,05), after application of glutapyrone by 1,18 times (р<0,05); in passive group – by 1,15 times (р<0,05), with correction – by 1,07 times (р<0,05) compared to the control. Thus, application of glutapyrone resulted to normalization of the activity of catalase and increasing of the content of ascorbic acid in the lungs tissues of both types of rats. We found that the rats from active group had more significant changes than the rats from passive groupe. Conclusions. At emotional-pain acute stress there is development of disbalance between the action of prooxidant and antioxidant systems due to the activation of free radical processes. The development of oxidative stress and reduction of antioxidant defense were more pronounced in active type rats. This indicates that stress lung damage also depends on the typological features of the nervous system. Experimental correction by glutapyrone leads to reduce of pathological changes in rats lungs at acute emotional-pain stress. Prospects for further research. Further study of the pathological changes in the lungs tissues under condition of acute emotional-pain stress in dynamics