Електрофоретична активність клітин букального епітелію в дітей із дисбактеріозом кишечнику

Abstract

Зміна нормальної мікрофлори кишкового тракту при дисбактеріозах може призводити до функціональних змін у кишечнику, що зумовлює порушення всмоктування поживних речовин, засвоєння кальцію, синтез ряду вітамінів, а це шкідливо діє на формування твердих тканин зубів. Під час проведення дослідження нами було виявлене підвищення показників електрофоретичної активності клітин букального епітелію у дітей із дисбактеріозом кишечника. Изменение нормальной микрофлоры кишечного тракта при дисбактериозах может приводить к функциональным изменениям в кишечнике, что приводит к нарушению всасывания питательных веществ, усвоение кальция, синтез ряда витаминов, а это вредно действует на формирование твердых тканей зубов. Во время проведения исследования нами было обнаружено повышение показателей электрофоретической активности клеток буккального эпителия у детей с дисбактериозом кишечника; In recent years, an increasing number of children with caries in early childhood. This is due to many factors, one of which is a child of the disease in the first years of life. Currently, increased the number of intestinal diseases, the cause of which was a violation of the qualitative and quantitative ratio of aerobic and anaerobic microorganisms in the lumen of the body. This disease is called "intestinal dysbiosis". Changing the normal intestinal microflora at dysbacteriosis may lead to functional changes in the bowel, leading to malabsorption of nutrients, calcium absorption, synthesis of some vitamins and a harmful effect on the formation of dental hard tissues. Recently, the question is widely studied biological significance of the properties of the cell nuclei. Revealed nuclear cell potential that is different from and represents a cytoplasmic genetic and functional differences between the state of cells. In this regard, the aim of our study was to investigate the activity of the cells of buccal epithelium: percentage of motile cell nuclei, nuclei, and the displacement amplitude plazmolem, amplitude ratio plazmolem and nuclei in children with intestinal dysbiosis. For the study we selected 40 children 2-3 years old with intestinal dysbiosis (study group) and clinically healthy children of the same age (40), who were in the control group. Our observations have shown that at a time when the negatively charged cell nuclei moving buccal epithelium is always moving in the cytoplasm to the anode, mobile plasmolemma moved in the opposite direction - to the cathode. This indicates that the total charge on the surface of the movable plazmol СBE contained settled tap water or standard phosphate buffer pH=7 with calcium, is positive. Electrophoretic buccal epithelial cell activity in children with intestinal dysbiosis differ from the healthy children. We found that the percentage of motile cell nuclei and plazmol dysbiosis in children with lower than in healthy children. In children 2 years, all were lower than in children 3 years of age. Thus, the observed increase in activity indicators electrophoretic buccal epithelial cells in the age aspect. In children with intestinal dysbiosis, compared with healthy children showed a reduction in percentage of motile cells of buccal epithelium is 4,5 ± 0,19% (5,6 ± 0,15% - in the comparison group), in the ratio of amplitude and amplitude plasmolemma cell nuclei buccal epithelium to 1.37 (- 1.5 in the control group), indicating a decrease in defense reactions in the mouth, which in turn creates a cariogenic situation in the mouth, which calls for preventive measures aimed at its elimination. During the research we have found no increase in the electrophoretic activity buccal epithelial cells in children with intestinal dysbiosis. Number of cell nuclei are displaced in the electric field is determined by the physiological state of the organism, the age of the child and homeostasis mouth that must be considered when planning and carrying out preventive measures aimed at increasing the resistance of dental hard tissues.