Fouzan, MohammedMustafa, HananEntonu, PeterTkachenko, О. V.Ткаченко, Олена Вікторівна2025-06-302025-06-302025New molecular-biological and genetic methods applied in African countries / M. Fouzan, H. Mustafa, P. Entonu ; scientific supervisor : O. V. Tkachenko // XХІX Конгрес студентів та молодих учених «Майбутнє за наукою», 9–11 квітня 2025 р., м. Тернопіль, Україна. – Тернопіль, 2025. – С. 96.https://repository.pdmu.edu.ua/handle/123456789/26630Innate pathology level in Africa exceeds high-income countries on 20%. Hemoglobinopathies, G6PD deficiency, albinism, neural tube defects are prevalent in sub-Saharan Africa. Nigerian population having sickle-cell disease is the biggest in the world, comprising 22-25% of all the inhabitants. 2:100 of Nigerian children or 2- 3% of all newborns have it. Muscular-skeletal and gastro-intestinal congenital anomalies are prevalent in the Nigerians among all congenital diseases. Inherited myopathies were found to be dominant in the North Africa. There exist dys- advantages of old investigative methods defining essentiality to create, introduce in healthcare system and to manage the new ones – informative, rather easily to be performed and possessing multiple advantages. The authors analyzed modern literature concerning new investigative methods for medical-biological and genetic researches in African countries. Materials and methods: scientific literary sources for 2019-2025 on the topic studied. Brief analysis of literary sources testifies to their rather big set and expressed opportunities. Following methods application was analyzed: Next-Generation Sequencing (NGS), Single Nucleotide Polymorphism (SNP) Genotyping, Sanger Sequencing, Fluorescence in situ Hybridization (FISH), Restriction Fragment Length Polymorphism (RFLP), Microarray Analysis, CRISPR- Cas9 gene editing, Reverse Transcription PCR (RT-PCR), Whole Exome Sequencing (WES).enmolecular biologygeneticsmolecular-biological methodsgenetic methodsnext-generation sequencing (NGS)single nucleotide polymorphism (SNP) genotypingsanger sequencingfluorescence in situ hybridization (FISH)restriction fragment length polymorphism (RFLP)microarray analysisCRISPR-Cas9 gene editingreverse transcription PCR (RT- PCR)whole exome sequencing (WES)whole-genome sequencingidentifying mutationschromosomal abnormalities detectinggenetic diversitypopulational geneticssickle cell anemiaspecific genes’ sequencinginfectious diseases diagnosticsgenetic variations’ analyzing at specific positions in the genomedisease susceptibilitymalariapharmacogenomicsgenetic disorderscancer markersHIVleukemiathalassemiaRNADNAhepatitisCOVID-19reverse transcriptionrevertasereverse polymeraserare inherited diseasesexonsexomescomplex diseasesdiabeteshypertensionhemoglobinopathiesG6PD deficiencyalbinismneural tube defectsmuscular-skeletal anomaliesgastro-intestinal congenital anomaliesinherited myopathiescaspasescaspase 9Thesis