Процеси перекисного окиснення ліпідів у легенях щурів за умов опікової хвороби та їх корекція препаратом «Ліпін»

Abstract

В умовах опікової хвороби в тканинах легень підвищується активність процесів ПОЛ на фоні зниження антиоксидатного захисту. Експериментальна корекція препаратом «Ліпін» сприяє нормалізації патологічних змін в легенях щурів за умов опікової хвороби, на що вказує підвищення активності антиоксидантних ферментів та пригнічення вільно-радикального окиснення; В условиях ожоговой болезни в тканях легких повышается активность процессов ПОЛ на фоне снижения антиоксидантной защиты. Экспериментальная коррекция препаратом Липин способствует нормализации патологических изменений в легких крыс в условиях ожоговой болезни, на что указывает повышение активности антиоксидантных ферментов и угнетение свободно-радикального окисления; Introduction. It is known that burn disease leads to oxidative stress. Lung injury is an important component in the structure of multiple organ failure syndrome at burn disease. Better understanding of the mechanisms of pathological changes in burn disease contributes to searching for effective methods of treatment. Objective. The research was aimed to study the influence of «Lipin» on pathological changes in rats lungs under modelled burn disease. Materials and methods. Experiments were carried out on 112 white male rats-, weight 180-200g. The hind limbs of rats were exposed to + 75°C bath for 7s to induce experimental burn disease. The "Lipin" injected intraperitoneally (5 mg/kg) after burn. In homogenate of lung tissue were determined the content of malondialdehyde (MDA), oxidation-modified proteins (OMP) and middle mass molecules (MMM), superoxide dismutase (SOD), catalase, phospholipids, triglycerols, free cholesterol and fat acids on the 1, 7, 14, 21, 28 days of burn disease. Results. We found that the level of MDA in the lungs of rats increased compared with the control in 3.12 times at 24 h, on the seventh day - in 2.73 times, indicating activation of free radical processes. At 24 h increase of OMP in lung tissues compared with the control was in 1.58 times on the seventh day - in 1.74 times. These changes indicated the accumulation of free radical oxidation in the lungs. It also leads to endogenous intoxication and increase content MMM. Сontent of MMM in the lungs of rats increased in 1.72 times on the first day, the seventh day - in 2.09 times compared with the control. This shows the development of endotoxemia and metabolic disorders in the lungs of rats. When correction of "Lipin" level of MDA increased 1.84 times at 24 h, on the seventh day - in 1.31 times. On the first day increase of OMP amounted in 1.19 times, on the seventh day - in 1.32 times. Content of MMM in lung tissues of rats increased in 1.27 times at 24 h, on the seventh day - in 1.54 times compared with the control. Under conditions of burn disease is observed the decrease of phospholipids and triglycerols, the increase in free cholesterol and fat acids. Experimental correction by «Lipin» leads normalization of lipid composition in rats’ lungs at burn disease. Conclusions. Under the conditions of burn disease in lungs the activity of free-radical process increases while antioxidant defense decrease. Experimental correction by «Lipin» leads to the normalization of pathological changes in rats’ lungs at burn disease. Thus, the use of "Lipin" reduces the intensity of free radical processes and the degree of intoxication in lung tissues at burn disease