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Документ Dynamics of CD68 receptor expression in macrophages of the interstitial space of the rat testis under triptorelin prolonged administration(Ukr. Biochem. J., 2023) Stetsuk, Ye. V.; Shepytko, V. I.; Akimov, O. Ye.; Boruta, N. V.; Rud, M. V.; Pelypenko, L. B.; Lysachenko, O. D.; Vilkhova, O. V.; Skotarenko, T. A.; Voloshyna, O. V.; Стецук, Євген Валерійович; Шепітько, Володимир Іванович; Акімов, Олег Євгенович; Борута, Наталія Володимирівна; Рудь, Марія Володимирівна; Пелипенко, Лариса Борисівна; Лисаченко, Ольга Дмитрівна; Вільхова, Олена Вікторівна; Скотаренко, Тетяна АнатоліївнаTestosterone, the production of which is stimulated by the release of luteinizing hormone (LH) has a remarkable anti-inflammatory and immunomodulatory effect, and in conditions of testosterone deficiency tissue damage can occur due to excessive differentiation of macrophages into a proinflammatory M1 phenotype. The aim of this study was to determine the spread of CD68 receptors as the marker of inflammation on the cells in the interstitial space and testicular vessels under LH synthesis blockade with tryptorelin. Sexually mature white male rats were randomly divided into control (10 animals) and experimental (25 animals) groups. Animals in the experimental group were injected with a tryptorelin acetate solution (0.3 mg/kg). Immunochemical analysis of CD68+ expression was estimated at Olympus FV10i-LIV laser scanning confocal microscope using fluorescent labeling dye. It was shown that luteinizing hormone deprivation led to an increase in the distribution of the CD68 receptor in the interstitial space and in the testicular vessels from day 30 to 180 of the experiment, associated with the increase of inducible NO synthase activity in testis tissue.Документ Role of nitric oxide in development of fibrotic changes in rats’ testes after 270 day central deprivation of testosterone synthesis(Українська медична стоматологічна академія, 2020) Stetsuk, Ye. V.; Akimov, O. Ye.; Shepitko, K. V.; Boruta, N. V.; Goltsev, A. M.; Стецук, Евгений Валерьевич; Акимов, Олег Евгеньевич; Шепитько, Константин Владимирович; Борута, Наталия Владимировна; Гольцев, Анатолий Николаевич; Стецук, Євген Валерійович; Акімов, Олег Євгенович; Шепітько, Костянтин Володимирович; Борута, Наталія Володимирівна; Гольцев, Анатолій МиколайовичDisturbance in production of nitric oxide (NO) may lead to various changes in different organs and systems. Certain clinical situations require prolonged usage of inhibitors of testosterone synthesis. Scientific literature provides limited information regarding the influence of prolonged deprivation of testosterone synthesis on production of NO and microscopic organization of rats’ testes. Prolonged central deprivation of testosterone synthesis leads to endothelial dysfunction, development of fibrosis, decreases the nitric oxide production and shifts pro-/antioxidant balance in favor of the pro-oxidants without increase in lipid peroxidation intensity. Central deprivation of testosterone synthesis leads to fibrosis with subsequent disruption of the structural organization of the convoluted seminiferous tubules, hemodynamic disturbances, endothelial dysfunction, increased density of the vascular wall of blood vessels and systemic stasis. Decreased production of NO from constitutive isoforms of NO-synthase plays major role in development of structural changes in the interstitial tissue of testes on the 270th day of the experiment.