Кафедра біології
Постійне посилання на фонд
Переглянути
Перегляд Кафедра біології за Автор "Akimov, O. Ye."
Зараз показуємо 1 - 7 з 7
Результатів на сторінці
Налаштування сортування
Документ Changes in the microcirculatory bed of the liver during alcoholic hepatitis modeling(Полтавський державний медичний університет, 2023) Mykytenko, A. O; Akimov, O. Ye.; Yeroshenko, G. A.; Shevchenko, O. M.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Шевченко, О. М.; Непорада, Каріне СтепанівнаIt is well known that chronic alcohol consumption leads to multiple organ damage and microcirculation disorders of organs and tissues. The aim of the work was to study the morphometric indicators of the microcirculatory bed of rat liver during alcoholic hepatitis modeling. The experiments were performed on 30 white, mature male Wistar rats, weighing 180–220 g. The animals were divided into 2 groups: I – control; group II– animals, on which we modeled alcoholic hepatitis according to Yu.M. Stepanov. (2017). The diameter of the sinusoidal capillaries around the central vein and the hepatic triad was increased at all time points compared to the control. The diameter of the lumen of the central vein of the hepatic lobe of rats increased on the 1st, 5th and 7th day of the experiment. The diameter of the lumen of the interlobular artery of rats was decreased during the studied periods, and the interlobular vein was increased. The diameter of the lumen of the arteriole increased on the 3rd day of the experiment, and the diameter of the lumen of the venule of the lobule of the rats decreased on the 1st day of the experiment. The microcirculatory channel of the liver of rats under the conditions of simulation of chronic alcoholic hepatitis in the first week demonstrates an increase in the exchange of metabolites between the central vein and the vein of the triad, which is accompanied by their expansion and threatens the development of venous hyperemia.Документ Extracellular matrix of rat liver under the conditions of combining systemic inflammatory response syndrome and chronic alcohol intoxication(Полтавський державний медичний університет, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне СтепанівнаThe aim of this study was to establish the effect of systemic inflammatory response syndrome on the background of chronic alcohol intoxication on the concentration of oxyproline, glycosaminoglycans and sialic acids in the liver of rats. The study was performed on 24 male Wistar rats. Animals were divided into 4 groups of 6 animals: I – control; II – systemic inflammatory response syndrome group; III – alcoholic hepatitis groupand IV – animals with a combination of systemic inflammatory response syndrome and chronic alcohol intoxication. The concentrations of glycosaminoglycans, heparin-heparan, keratan-dermatan and chondroitin fractions of glycosaminoglycans, free oxyproline and sialic acids were studied in the liver tissue homogenate. The combination of systemic inflammatory response syndromeand chronic alcohol intoxication leads to ncreased intensity of collagenolysis, glycoprotein catabolism and reduced intensity of breakdown of proteoglycans of connective tissue of the liver. However, it changes the ratio of individual fractions of glycosaminoglycans in the direction of reducing тtheconcentration of anti-inflammatory and increasing regenerative.Документ Hemical changes in the extracellular matrix of rat liver during chronic alcohol intoxication(Полтавський державний медичний університет, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Shevchenko, O. M.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Шевченко, Олена Миколаївна; Непорада, Каріне СтепанівнаExcessive alcohol consumption is a global health problem worldwide, resulting in more than 3 million deaths each year. The purpose of this work was to study the biochemical markers of intercellular matrix metabolism of rat liver under the conditions of chronic alcohol intoxication modeling. The experiments were performed on 30 male Wistar rats, weighing 180-220 g. The animals were divided into 2 groups: І – control; ІІ – chronic alcohol intoxication. Animals were removed from the experiment on days 10, 14, 21 and 28. We studied concentration of glycosaminoglycans, oxyproline and sialic acids in rat liver. We established that the greatest depolymerization of proteoglycans was on the 21st day, the highest intensity of collagenolysis was on the 14th day. Glycoproteins underwent the greatest catabolism on the 28th day. Chronic alcohol intoxication on the 28th day led to increased breakdown of glycoproteins and proteoglycans of the extracellular matrix of the liver, and increased the intensity of collagenolysis.Документ Influence of NF-kB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication(Інститут біохімії ім. О. В. Палладіна НАН України, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне СтепанівнаAlcohol-related liver disease is the most common cause of liver disease worldwide. The purpose of this work is the establishment of the influence of the transcription factor κB on the development of oxidative nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication.Chronic alcohol intoxication led to increased production of reactive oxygen and nitrogen species on the background of decreased antioxidant activity, thus intensifying lipid peroxidation in the liver. Blockade of the transcription factor κB during chronic alcohol intoxication despite an increase in antioxidant activity and decrease of reactive oxygen and nitrogen species production did not ameliorate oxidative damage to the liver. Blockade of activation of nuclear transcription factor κB in rat liver by PDTC reduced the risk of oxidative damage to hepatocytes, but did not reduce the risk of developing nitrosative damage to hepatocytes.Документ Influence of transcription factor κB on remodeling of extracellular matrix of rat liver under conditions of chronic alcohol intoxication(Полтавський державний медичний університет, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне СтепанівнаLiver fibrosis is a common tissue reaction associated with chronic liver damage resulting from prolonged parenchymal cell damage and inflammation. The fibrogenic response is characterized by the progressive accumulation of extracellular matrix components enriched in collagen fibrils and impaired extracellular matrix remodeling. The aim of this study was to establish the effect of transcriptional factor κB on the concentration of oxyproline, glycosaminoglycans and sialic acids in the liver of rats under conditions of chronic alcohol intoxication. The study was performed on 24 male Wistar rats. The animals were divided into 4 groups of 6 animals: І – control; II – animals with inhibition of κB; ІІI – animals with chronic alcohol hepatitis and ІV – animals with κB inhibition on the background of chronic alcohol intoxication. We studied the total concentration of glycosaminoglycans, concentration of heparin-heparan, keratan-dermatan and chondroitin fractions of glycosaminoglycans, free oxyproline and sialic acids in the liver tissue homogenate. Prolonged alcohol intoxication of rats leads to remodeling of the extracellular matrix as evidenced by increased collagenolysis and catabolism of glycoconjugates of amorphous connective tissue of the liver. The use of the inhibitor of the transcription factor κB in the background of prolonged alcohol intoxication of animals reduced the concentration of GAG by 1.43 times, lowered oxyproline concentration by 1.6 times and decreased the concentration of sialic acids by 1.14 times compared to chronic alcohol intoxication group. Transcriptional factor NF-κB under conditions of chronic alcohol intoxication affects the architectural remodeling of connective tissue of rat liver by increasing degradation of collagen fibers and destruction of glycoproteins and proteoglycans of the extracellular matrix.Документ Morphological and functional changes of the hepatic vascular bed Under the conditions of modeling alcoholic hepatitis(Полтавський державний медичний університет, 2021) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне СтепанівнаThe experiments were performed on 30 white adult outbred male rats weighing 180–220 g. Animals were divided into 2 groups: I – control (n=6); II – animals with simulated alcoholic hepatitis (n=24). We studied the total NO synthase activity, peroxynitrite, nitrites and nitrosothiols concentrations in rat liver homogenate. We also studied morphometric parameters of hemomicrocirculatory tract of the rat liver. On the 7th day of modeling of alcoholic hepatitis interparticle arteries and veins and lobular arterioles narrow. The central vein dilates. The sinusoidal capillaries around the hepatic triad and central vein dilate. Alcoholic hepatitis increases the total activity of NO synthases, the concentration of peroxynitrites and nitrosothiols, but reduces the concentration of nitrites in the liver of rats on day 7 of the experiment. Nitric oxide and its metabolites play an important role in the regulation of the resistant part of the hemomicrocirculatory tract of the rat liver in the first three days of the experiment. Further redistribution of nitric oxide cycle metabolites leads to an increase in the concentration of peroxynitrite, which is a factor of secondary alteration; Експерименти виконані на 30 білих статевозрілих безпородних щурах-самцях, вагою 180-220 г. Тварини були розділені на 2 групи: І – контрольна (n=6); ІІ група – тварини, яким моделювали алкогольний гепатит (n=24). В гомогенаті печінки щурів визначали активність загальної NO-синтази, концентрації пероксинітриту, нітритів та нітрозотіолів. Також визначали морфометричні параметри емомікроциркуляторного русла печінки щурів. На 7 добу моделювання алкогольного гепатиту міжчасточкові артерії і вени, часточкові артеріоли звужуються. Центральна вена розширюється. Синусоїдні капіляри навколо печінкової тріади і центральної вени розширяються. Алкогольний гепатит збільшує загальну активність NO-синтаз, концентрацію пероксинітритів та нітрозотіолів, проте знижує концентрацію нітритів у печінці щурів на 7 добу експерименту. Оксид азоту і його метаболіти грають важливу роль в регуляції резистентної ланки гемомікроциркуляторного русла печінки щурів перші три доби експерименту, подальший перерозподіл метаболітів циклу оксиду азоту призводить до збільшення концентрації пероксинітриту, який виступає фактором вторинної альтерації.Документ Peculiarities of connective tissue degradation in rat’s liver on early terms of chronic alcoholic hepatitis modelling(Українська медична стоматологічна академія, 2021) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина АнатоліївнаThe article deals with the peculiarities of connective tissue degradation in rat liver on early terms of chronic alcoholic hepatitis modelling. Modeling of chronic alcoholic hepatitis leads to the development of oxidative stress in rat liver tissues due to a decrease in the activity of antioxidant enzymes and an increase in the production of reactive oxygen species. As a result of the development of oxidative stress in the liver of rats in the first stages of modeling alcoholic hepatitis, the concentration of the anti-inflammatory heparin-heparan fraction of glycosaminoglycans decreases, the accumulation of keratan-dermatan and chondroitin fractions of glycosaminoglycans occurs. The processes of degradation of the fibrous components of the liver’s connective tissue increase and the concentration of the sulfide anion decreases.