Кафедра медицини катастроф та військової медицини

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https://repository.pdmu.edu.ua/handle/123456789/25

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Перегляд Кафедра медицини катастроф та військової медицини за Автор "Akimov, O. Ye."

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  • Ескіз недоступний
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    Influence of prolonged tripterelin-induced central deprivation of testosterone synthesis on morphological structure of rat’s liver
    (Українська медична стоматологічна академія, 2021) Polyvyana, O. A.; Shepitko, K. V.; Stetsuk, Ye. V.; Akimov, O. Ye.; Dubinin, D. S.; Полив'яна, Олександра Анатоліївна; Шепітько, Костянтин Володимирович; Стецук, Євген Валерійович; Акімов, Олег Євгенович; Дубінін, Дмитро Сергійович
    In recent years, the incidence of prostate cancer has increased worldwide. For example, in 2012, the GLOBOCAN project found that prostate cancer was the second most commonly diagnosed cancer and the fifth leading cause of cancer deaths among men worldwide. Insufficient testosterone can exacerbate liver damage caused by obesity. The aim of our study was to identify morphological changes in the liver of rats at the tissue and cellular levels, to study the processes of formation of reactive oxygen species and the intensity of lipid peroxidation during prolonged central deprivation of testosterone synthesis caused by triptereline. Triptorelin-induced central deprivation of testosterone synthesis leads to oxidative damage to hepatocytes due to increased production of reactive oxygen species and decreased activity of antioxidant enzymes. Oxidative damage to liver cells begins at the molecular and cellular levels and becomes apparent at the tissue level on day 180 of the central deprivation of testosterone synthesis.
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    Документ
    Morphofunctional features of rat testes interstitial endocrinocytes and sustentocytes after 90 days of central testosterone synthesis deprivation
    (Українська медична стоматологічна академія, 2020) Stetsuk, Ye. V.; Akimov, O. Ye.; Shepitko, K. V.; Goltsev, A. N.; Стецук, Євген Валерійович; Стецук, Евгений Валерьевич; Акімов, Олег Євгенович; Акимов, Олег Евгеньевич; Шепітько, Костянтин Володимирович; Шепитько, Константин Владимирович; Гольцев, Анатолій Миколайович; Гольцев, Анатолий Николаевич
    With the social system development, there is a tendency to change attitudes towards family and family values. In developed European countries, there is a trend towards high sexual activity in elderly men and late creation of a family with children, which undergoes certain difficulties in connection with a decreased testosterone production in later years. The purpose of the study was to establish the microscopic organization of rat interstitial endocrinocytes and sustentocytes, to determine the sources of nitric oxide production and the intensity of oxidative stress in the testes with experimental central deprivation of testosterone synthesis with diphereline on the 90th day of the experiment. The experiments were carried out on 20 sexually mature male white rats of the Wistar line. Rats were divided into 2 groups: the control group (10) and the experimental group (10), which were injected subcutaneously with diphereline (Triptorelin embonate) at a dose of 0.3 mg / kg of the active substance for 90 days. Prolonged central deprivation of testosterone synthesis in animals leads to emergence of functional stress structural signs in population of sustentocytes and interstitial endocrinocytes, which are aimed to support testicular secretion. Central deprivation of testosterone synthesis within 90 days causes oxidative stress development owing to reactive oxygen species hyperproduction and nitrite accumulation in testicular tissue due to increased inducible NO-synthase activity;
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    Документ
    Structural organization of stromal and parenchymal components of rat testes during central deprivation of testosterone synthesis on the 180 day of the experiment
    (Українська медична стоматологічна академія, 2020) Стецук, Євген Валерійович; Стецук, Евгений Валерьевич; Stetsuk, Ye. V.; Акімов, Олег Євгенович; Акимов, Олег Евгеньевич; Akimov, O. Ye.; Шепітько, Костянтин Володимирович; Шепитько, Константин Владимирович; Shepitko, K. V.; Гольцев, Анатолій Миколайович; Гольцев, Анатолий Николаевич; Goltsev, A. M.
    Prolonged central deprivation of testosterone synthesis may lead to biochemical and morphological changes in testes. Influence of prolonged testosterone deficiency on reactive nitrogen and oxygen species production, morphological changes in interstitial endocrinocytes and sustentocytes is not yet fully understood. The number of interstitial endocrinocytes is reduced in comparison with the control group, there are interstitial spaces between the convoluted tubules with a complete absence of interstitial endocrinocytes. Interstitial endocrinocytes are reduced in size, their nuclei are heterochromic. When we studied the structural organization of rat sustentocytes from the experimental group in comparison with the control group, we found that hyperplasia of the elements of the smooth endoplasmic reticulum was observed in the cytoplasm of the sustentocytes. The number of mitochondria in the cytoplasm of the sustentocytes decreased, the electron density of the mitochondrial matrix was lowered, protein structures were present either inside the vacuoles or independently located in the cell cytoplasm. Biochemical studies revealed increased NO production from inducible NO-synthase and development of oxidative stress. Experimental central deprivation of testosterone synthesis with diphereline on the 180th day of the experiment leads to shift of NO synthesis from constitutive NO-synthases to inducible NO-synthase and intensification of oxidative stress due to increase of superoxide anion-radical production and decrease in antioxidant protection.