Перегляд за Автор "Kovtun, S. I."
Зараз показуємо 1 - 3 з 3
Результатів на сторінці
Налаштування сортування
Документ NOS3 (rs61722009) gene variants testing in prediction of COVID-19 pneumonia severity(Elsevier, 2023) Fishchuk, L. Ye.; Rossokha, Z. I.; Pokhylko, V. I.; Cherniavska, Yu. I.; Dubitska, O. M.; Vershihora, V. O.; Tsvirenko, S. M.; Kovtun, S. I.; Horovenko, N. H.; Похилько, Валерій Іванович; Чернявська, Юлія Ігорівна; Цвіренко, Світлана МиколаївнаBackground There is a hypothesis that a sufficient level of endothelial nitric oxide synthase is important for reliable protection against COVID-19. Theoretical ideas about the NOS3 gene demonstrated that it can have an effect on links of the complications pathogenesis in COVID-associated pneumonia. We determined the goal – to investigate the association of the NOS3 gene variants with the occurrence of the disease and its clinical course in patients of the intensive care unit. Methods The study group included 117 patients with a diagnosis of severe “viral COVID-19 pneumonia”. Determination of NOS3 gene variants was performed using the PCR method. The probability of differences in the quantitative results were determined using ANOVA or Kruskal-Wallis test (depends on normality of studied parameters). Results Our results indicate that the presence of the NOS3 gene 4a allele increase the risk of complicated COVID-19-associated pneumonia (χ2 = 18.84, p = 0.00001, OR = 3.53 (1.95–6.39)). It was shown, that carriers of the 4aa genotype had a significantly higher ratio of SpO2/FiO2 on the first and second days after hospitalization (p = 0.017 and p = 0.03, respectively). Patients with the 4aa genotype also had the acid-base imbalances, as showed by indicators of base deficiency and standard bicarbonate, which were beyond the reference values. Potassium and sodium concentrations on the first and second day after hospitalization were also significantly lower in patients with 4aa genotype (p = 0.009 and p = 0.048, respectively), for whom, in the same time, the concentrations of C-reactive protein and total bilirubin were significantly higher (p = 0.002 and p = 0.033, respectively). Conclusions Our results confirmed that the rs61722009 variant of the NOS3 gene is associated with an increased risk of severe СOVID-19-associated pneumonia and its adverse clinical course with potential progression of kidney and liver damage, and occurrence risk of systemic inflammatory response syndrome. These results require further research for the new metabolic strategy formation, in order to prevent the severe COVID-19 associated pneumonia and its complications.Документ The deletion variant of the CCR5 gene (rs333) but not the ACE gene (rs4340) is associated with long-term respiratory support in patients with COVID-19 pneumonia(ТОВ "МОРІОН", 2020) Rossokha, Z. I.; Fishchuk, L. Ye.; Pokhylko, V. I.; Cherniavska, Yu. I.; Tsvirenko, S. M.; Kovtun, S. I.; Medvedieva, N. L.; Vershyhora, V. O.; Gorovenko, N. G.; Россоха, Зоя Іванівна; Фіщук, Лілія Євгенівна; Похилько, Валерій Іванович; Чернявська, Юлія Ігорівна; Цвіренко, Світлана Миколаївна; Ковтун, Сергій Іванович; Медведєва, Наталія Леонідівна; Вершигора, Вікторія Олександрівна; Горовенко, Наталія Григорівна; Россоха, Зоя Ивановна; Фищук, Лилия Евгеньевна; Похилько, Валерий Иванович; Чернявская, Юлия Игоревна; Цвиренко, Светлана Николаевна; Ковтун, Сергей Иванович; Медведева, Наталия Леонидовна; Вершигора, Виктория Александровна; Горовенко, Наталья ГригорьевнаThe aim was to analyze the effect of the ACE gene (c.2306-117_404I/D, rs4340) and the CCR5 gene (del32, rs333) variants on the course of severe COVID-19 pneumonia in patients treated at the intensive care unit. Materials and methods. The study group included 31 patients (16 men and 15 women) with diagnosis «viral COVID-19 pneumonia». All patients underwent standard daily repeated clinical, instrumental and laboratory examinations. Determination of the ACE and CCR5 gene variants was carried out by a molecular method using allele-specific polymerase chain reaction. Results. The results of our retrospective study did not confirm the association of investigated genes variants with lethal outcomes. Clinical predictors of lethal outcomes were: low of SpO2/FiO2 ratio, tachypnea, tachycardia, low systolic blood pressure, anemia, leukocytosis during the first days of hospitalization and need of mechanical lung ventilation. Patients with heterozygous W/del32 genotype of the CCR5 gene in comparison with patients with genotype W/W had significantly longer respiratory support, namely a significantly increased duration of oxygen therapy using an oxygen mask (4.50±3.70 vs. 2.19±1.28 days, respectively), significantly longer mechanical lung ventilation (15.00±4.24 vs. 4.40±4.98 days, espectively) and the significantly greater total duration of oxygen therapy (9.60±5.68 vs. 4.19±3.84 days, respectively). Patients with the W/del32 genotype of the CCR5 gene had significantly increased white blood cell counts as compared to patients with the W/W genotype (13.64±10.66 vs. 8.38±2.85, respectively). Conclusions. Significant clinical predictors of lethal outcomes in patients with severe COVID-19 pneumonia were found on admission: lower SpO2/FiO2 ratios, tachypnea, tachycardia, anemia, leukocytosis during the first days of hospitalization, and need of mechanical lung ventilation. The variant of the CCR5 gene was the genetic predictor of severe course of COVID-19 pneumonia with increased need for respiratory support. The variant of the CCR5 gene was associated with elevated white blood cell count in the complete blood test.The obtained results indicate the need for further multifaceted research in this direction to determine the leading genetically mediated pathogenetic mechanisms of severe viral COVID-19 pneumonia.Документ Інфекційні полінейропатії у дітей(Вищий державний навчальний заклад України «Українська медична стоматологічна академія», 2012) Пікуль, Катерина Вікторівна; Ковтун, Сергій Іванович; Гасюк, Наталія Іванівна; Прилуцький, Костянтин Юрійович; Ладур, Тетяна Сергіївна; Пикуль, Екатерина Викторовна; Ковтун, Сергей Иванович; Гасюк, Наталия Ивановна; Прилуцкий, Константин Юрьевич; Ладур, Татьяна Сергеевна; Pikul, K. V.; Kovtun, S. I.; Gasyuk, N. I.; Prilutsky, K. Y.; Ladur, T. S.У статті авториприводять клінічний приклад дитини з діагнозом: Хвороба Лайма (бореліоз), стадія дисемінації, тяжкий перебіг. Правобічний неврит лицевого нерва, правосторонній лагофтальм, синдром сухого ока справа. Герпес VI типу. Метаболічна кардіоміопатія. Діагональна хорда в порожнині лівого шлуночка. Вторинна недостатність імунітету. Гострий бронхіт (реконвалісцент); Актуальность проблемы обусловлена особенностью внимания к клинико-эпидемиологическому наблюдению за болезнями, сопровождающимися вялыми параличами у детей до 15 лет. Лечение инфекционных полинейропатий является сложным процессом ввиду полиэтиологичности, разнообразия патогенетических механизмов и регенераторных изменений в нервных волокнах, а также вариабельности клинических проявлений. В статье авторы, помимо обзора литературы, приводят клинический случай у ребенка с диагнозом: Болезнь Лайма (борелиоз), стадия диссеминации, тяжелое течение. Правосторонний неврит лицевого нерва, правосторонний логофтальм, синдром сухого глаза справа. Герпес VI типа. Метаболическая кардиомиопатия. Диагональная хорда в полости левого желудочка. Вторичная недостаточность иммунитета. Острый бронхит (реконвалесцент); Actuality of problem is caused by the peculiarity of attention to clinical and epidemiological surveillance for the diseases that are accompanied by flaccid paralysis of children under 15 years. Treatment of infectious polyneuropathy is complex process due to polyetiology, variety of pathogenic mechanisms and regenerative changes in the nerve fibers and also variability of clinical manifestations. In the article, except literature review, authors also lead clinical case of the disease of child with a diagnosis: Layme’s disease (borreliosis), dissemination stage, heavy flow. Right-sided neuritis of facial nerve, rightsided logophtalma, right-sided syndrome of dry eye. Herpes of VI type. Metabolic cardiomyopathy. Diagonal chord at the cavity of the left ventricle. Secondary deficiency of immunity. Acute bronchitis (reconvalescents).