Перегляд за Автор "Nazarchuk, O."

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  • Ескіз недоступний
    Документ
    CCL2/MCP-1 Serum Chemokine Level in Patients with Odontogenic Infectious and Inflammatory Diseases of the Soft Tissues of the Maxillofacial Area and Mediastinum
    (Acta Facultatis Medicae Naissensis, 2022) Faustova, M.; Nazarchuk, O.; Dmytriiev, D.; Avetikov, D.; Loban, G.; Babina, Yu.; Ananieva, M.; Фаустова, Марія Олексіївна; Аветіков, Давид Соломонович; Лобань, Галина Андріївна; Ананьєва, Майя Миколаївна
    Aim. The paper was aimed at determining the CCL2/MCP-1 level in patients with odontogenic infectious and inflammatory diseases of soft tissues of the maxillofacial area and mediastinum. Methods. The study involved 46 patients with odontogenic infectious and inflammatory diseases of soft tissues of the maxillofacial area and 12 healthy volunteers. The level of patients’ plasma CCL2/MCP-1 level was determined using a kit for specific ELISA.
  • Ескіз недоступний
    Документ
    Microbiological aspects concerning the etiology of acute odontogenic inflammatory diseases in the soft tissues of the head and neck region
    (2022) Avetikov, D.; Ananieva, M.; Faustova, M.; Nazarchuk, O.; Chumak, Yu.; Havryliev, V.; Loban’, G. ; Фаустова, Марія Олексіївна; Назарчук, Олександр Адамович; Лобань, Галина Андріївна; Чумак, Юлія Вікторівна; Аветіков, Давид Соломонович; Ананьєва, Майя Миколаївна; Гаврільєв, Виктор Миколайович
    Odontogenic purulent inflammatory diseases (OPIDs) make up about 20% of cases in the structure of general surgical pathology and are among the frequent diseases of the head and neck region with a high (10−40%) mortality rate. Insufficient information about the source state of acute odontogenic inflammation of the perimandibular soft tissues significantly reduces the effectiveness of diagnostic measures of OPID in head and neck region, as evidenced by almost 50% of the diagnostic error rate. Statistically, OPID in soft tissue of head and neck region most often occurs due to dissemination of pathogens of the necrotized pulp, periodontal pockets in periodontitis, or pericoronitis during the difficult eruption of retained teeth. Previously, the quantitative dominance (about 70%) of Staphylococcus spp. among the microorganisms isolated from the odontogenic foci of inflammation was determined. However, in recent years, with the expansion of microbiological diagnostic capabilities, the presence of non-fermenting Gramnegative bacteria and anaerobes with a significant proportional proportion of the total microbiota of OPID in soft tissue of head and neck region has been increasingly indicated. Recently, there has been a rapid acquisition of resistance of pathogens of odontogenic purulent inflammatory diseases of the maxillofacial region to various groups of antibiotics, which leads to ineffectiveness of their treatment and prompts the revision of existing protocols and treatment regimens in surgical dentistry.
  • Ескіз
    Документ
    The correspondence of the carbapenemase genotype and phenotypic antimicrobial profiles of Pseudomonas aeruginosa
    (Полтавський державний медичний університет, 2023) Bahniuk, N.; Faustova, M.; Riesbeck, K.; Prokopchuk, Z.; Paliy, V.; Nazarchuk, O.; Loban’, G.; Багнюк Н.; Фаустова, Марія Олексіївна; Ріесбек, К.; Прокопчук, З.; Палій, В.; Назарчук, Олександр Адамович; Лобань, Галина Андріївна
    The aimof the study was to determine the correspondence between the carbap-enemase genotype and the phenotypic antimicrobial profiles of P. aeruginosa.Materials and methods.The study included 51 clinical isolates of P. aeruginosa, isolated from the patients with post-operative complications of the respiratory or-gans. The final identification of the obtained isolates was performed in the Ries-beck laboratory using MALDI-ToF (Bruker), followed by the determination of their sensitivity to antimicrobial drugs at the EUCAST Development Laboratory (Växjö, Sweden). Determination of the resistance genes was carried out by using poly-merase chain reaction in real time (PCR-RF). The antimicrobial resistance index (ARI) was determined according to the method of G.V. de Socio. Statistical analysis was performed using the standard IBM SPSS Statistics software version 22.0 and GraphPad Prism Software 10.1.0. (USA, 2023).Results. 39 strains of P. aeruginosa (76.5%) showed polyresistance, and 26 of them (51.0%) were resistant to all antibiotics. According to research data, P. aeruginosa isolates most often carried the blaVIM gene. Genetically determined production of oxacillinase group B-lactamase class D among clinical isolates of P. aeruginosa occurred somewhat less often. Based on the obtained results, four carbapenemase genetic resistotypes of P. aeruginosa as pathogens of respira-tory tract complications in critically ill patients were established. We detected the antimicrobial resistance index (ARI) based on the phenotypic characteristics of P. aeruginosa at the level of 0.69+-0.39. The phenomenon of statistically reliable cor-relation of the ARI of microorganisms by phenotypic characteristics with their car-bapenemase genetic resistotypes was established.Conclusions. 76.5% of strains of P. aeruginosa show polyresistance, and 51.0% of them are resistant to all antibiotics. Four different carbapenemase genetic re-sistotypes of P. aeruginosa as pathogens of respiratory tract complications in critically ill patients were established. There is the phenomenon of statistically reliable correlation of the ARI of microorganisms by phenotypic characteristics with their carbapenemase genetic resistotypes.