Перегляд за Автор "Semaka, Oleksandr"

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  • Ескіз недоступний
    Документ
    Cytotoxicity of magnetite nanoparticles deposited in sodium chloride matrix and their functionalized analogues in erythrocytes
    (ELSEVIER, 2023) Lytvyn, Stanislav; Vazhnichaya, Elena; Kurapov, Yurii; Semaka, Oleksandr; Babijchuk, Lyubov; Zubov, Pavlo; Важнича, Олена Митрофанівна
    The synthesis of covered nanoparticles provides new properties to the materials for biomedical applications. This fully applies to iron oxide nanoparticles. The research aim was to study features of the magnetite nanoparticles synthesized by electron beam technology as well as to investigate their functionalization and cytotoxicity. Nanoparticle characteristics were determined by stan dard methods. Cytotoxiciy of nanoparticles was studied using erythrocyte model. It was shown that the original magnetite nanoparticles in the sodium chloride matrix can be functionalized with polyvinylpyrrolidone and ethylmethylhydroxypyridine succinate, an antioxidant. All investigated nanoparticles were non-toxic for erythrocytes at concentrations up to 100 μg Fe/ml. At 100-200 μg Fe/ml, they increased the amount of cells expressing phosphatidylserine on the outer membrane, the count of pathological forms of erythrocytes and hemolysis. These phe nomena were less pronounced if the nanosystem included the antioxidant. Therefore, magnetite nanoparticles can be obtained by electron beam technology and functionalized to form non-toxic nanosystems.
  • Ескіз недоступний
    Документ
    The influence of pure (ligandless) magnetite nanoparticles functionalization on blood gases and electrolytes in acute blood loss
    (Elsevier, 2023) Vazhnichaya, Elena; Lytvyn, Stanislav; Kurapov, Yurii; Semaka, Oleksandr; Lutsenko, Ruslan; Chunikhin, Alexander; Важнича, Олена Митрофанівна; Литвин, Станіслав Єгорович; Курапов, Юрій Анатолійович; Семака, Олександр Валентинович; Луценко, Руслан Володимирович; Чуніхін Олександр Юрійович
    Objective was to compare the effect of functionalization of magnetite (Fe3O4) nanoparticles (NPs) with sodium chloride (NaCl), or its combination with ethylmethylhydroxypyrydine succinate (EMHPS) and polyvinylpyrrolidone (PVP) on blood gases and electrolytes in acute blood loss. Ligandless magnetite NPs were synthesized by the electron beam technology and functionalized by mentioned agents. Size of NPs in colloidal solutions Fe3O4@NaCl, Fe3O4@NaCl@EMHPS, Fe3O4@NaCl@PVP, Fe3O4@NaCl@EMHPS@PVP (nanosystems 1–4) was determined by dynamic light scattering. In vivo experiments were performed on 27 Wistar rats. Acute blood loss was modeled by removal 25 % circulating blood. Nanosystems 1–4 were administered to animals intaperitoneally after the blood loss with followed determination of blood gases, pH and electrolytes. In blood loss, nanosystems Fe3O4@NaCl and Fe3O4@NaCl@PVP were able to improve the state of blood gases, pH, and the ratio of sodium/potassium in the blood. So, magnetite NPs with a certain surface modification can promote oxygen transport under hypoxic conditions.