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Документ Difficulties in Diagnosis of Sialolithiasis: A Case Series(Tokyo Shika Daigaku, 2018) Kolomiets, S.; Udaltsova, K.; Khmil, T.; Yelinska, A.; Pisarenko, O.; Shynkevych, V.; Коломієць, Світлана Веніамінівна; Удальцова, Кристина Олександрівна; Хміль, Тетяна Андріївна; Єлінська, Аліна Миколаївна; Писаренко, Олена Анатоліївна; Шинкевич, Вікторія ІгорівнаSialolithiasis is one of the most common and extensively obstructive disorders of the major salivary glands. Here, we report 3 cases of sialolithiasis in the submandibular salivary gland showing symptomatic similarities to other dental and non-dental disorders of the maxillofacial area. How the various clinical features of this condition and fndings on 3D-CT may lead to a misdiagnosis are also discussed. In the frst case, that of a 45-year-old woman, a fnal diagnosis of a non-radiopaque submandibular sialolith allowed the initially indicated surgical extraction of a malerupted and semi-impacted right mandibular 3rd molar to be abandoned. In the second case, that of a 57-year-old woman, radiographic fndings had previously led to a diagnosis of ameloblastoma, which had masked the presence of sialolithiasis for at least 9 years, despite the radiopacity of the sialolith. Meanwhile, exacerbation of sialolithiasis was mistaken for lymphadenitis. In the third case, that of a 40-year-old woman, sialolithiasis was diagnosed in a timely manner, despite the fact that the dentists’ attention had initially been focused on odontopathological symptoms. One feature of the present report is the concurrence of dental and non-dental pathologies affecting the same sextant as the sialolithiasis. Despite recent advances in imaging technology and diagnostics, cases of sialolithiasis being misdiagnosed continue to occur inclinical practice.Документ Effects of l-arginine and l-ornithine supplementations on the treatment of chronic periodontitis: A preliminary randomized short-term clinical trial(Elsevier Ltd., 2021-11-09) Shynkevych, V.; Kolomiiets, S.; Kaidashev, I.; Шинкевич, Вікторія Ігорівна; Коломієць, Світлана Веніамінівна; Кайдашев, Ігор ПетровичAbstract The growing interest in the possibilities of modulating macrophages in inflammatory diseases with therapeutic purpose has prompted the development of new approaches for the treatment of periodontitis. This randomized add-on open preliminary clinical study evaluated the short-term effects of L-arginine or L-ornithine as an adjuvant to scaling and root planing (SRP) in patients with chronic periodontitis. Materials and methods Seventy-five periodontitis patients were recruited and monitored clinically and immunologically at baseline (before SRP) and 30 ± 5 days after SRP. All patients were assigned by stratified randomization to SRP (SRP only, n = 25), Arg (SRP + L-arginine, n = 25) or Control (SRP + L-ornithine, n = 25) Group. The medicines were used according to available instructions for 10 and 15 days, respectively. During the study, all patients were on a stable diet, without changing their rations and regiments. As immunological monitoring immunohistochemical study of CD68+ and CD163 + single positive gingival macrophages for 5 patients per group in the same time-point was conducted. The data were statistically analyzed. Results Reduction of periodontal pocket depth (PPD) and bleeding on probing (BoP) was observed in all groups, with significant between-group differences for BoP in the Arg Group (p < 0.0001) at 30 days. The SRP and Arg groups demonstrated nonsignificantly increased density of CD68+ and CD163 + cells. The Orn Group showed an increase in the density of CD68+ and CD163 + macrophages at intragroup (p = 0.0066 and p < 0.0001) and between-group levels (p = 0.001 and p < 0.0001), and these changes corresponded to clinical PPD and BoP reduction. In the Arg and Orn groups at 30 days, CD163 + macrophages significantly predominated over CD68+ (p = 0.013, p < 0.0001). Conclusion The use of L-arginine and L-ornithine as an adjunct to SRP promotes additional limited immunological benefit in the treatment of periodontitis. Metabolic stimulation with L-ornithine, but not L-arginine, is preferable for CD163+ Mφs subpopulation in periodontitis-affected gingiva.Документ Long-Term Administration of Omeprazole-Induced Hypergastrinemia and Changed Glucose Homeostasis and Expression of Metabolism-Related Genes(2024-05-31) Kabaliei, A.; Palchyk, V.; Izmailova, O.; Shynkevych, V.; Shlykova, O.; Kaidashev, I.; Кабалєй, Аліна Вікторівна; Пальчик, Віталіна Вікторівна; Ізмайлова, Ольга Віталіївна; Шинкевич, Вікторія Ігорівна; Шликова, Оксана Анатоліївна; Кайдашев, Ігор ПетровичIntroduction. PPIs, or proton pump inhibitors, are the most widely prescribed drugs. There is a debate regarding the relationship between long-term PPI use and the risk of type 2 diabetes mellitus (T2DM). A potential connection between T2DM and PPIs could be an elevated gastrin concentration. This study is aimed at investigating the long-term effects of PPI omeprazole (OZ) on glucose homeostasis and pancreatic gene expression profile in mice. Methods. Healthy adult male BALB/c mice were randomly divided into three equal groups ( in each one): (1) experimental mice that received OZ 20 mg/kg; (2) control mice that received 30 μl saline per os; (3) intact mice without any interventions. Mice were treated for 30 weeks. Glucose homeostasis was investigated by fasting blood glucose level, oral glucose tolerance test (GTT), insulin tolerance test (ITT), and basal insulin resistance (HOMA-IR). Serum gastrin and insulin concentration were determined by ELISA. Expressions of Sirt1, Pparg, Nfκb1 (p105), Nfe2l2, Cxcl5, Smad3, H2a.z, and H3f3b were measured by RT-PCR. Result. The ROC analysis revealed an increase in fasting blood glucose levels in OZ-treated mice in comparison with control and intact groups during the 30-week experiment. A slight but statistically significant increase in glucose tolerance and insulin sensitivity was observed in OZ-treated mice within 30 weeks of the experiment. The mice treated with OZ exhibited significant increases in serum insulin and gastrin levels, accompanied by a rise in the HOMA-IR level. These animals had a statistically significant increase in Sirt1, Pparg, and Cxcl5 mRNA expression. There were no differences in β-cell numbers between groups. Conclusion. Long-term OZ treatment induced hypergastrin- and hyperinsulinemia and increased expression of Sirt1, Pparg, and Cxcl5 in mouse pancreatic tissues accompanied by specific changes in glucose metabolism. The mechanism of omeprazole-induced Cxcl5 mRNA expression and its association with pancreatic cancer risk should be investigated.Документ PPAR-γ agonist pioglitazone restored mouse liver m RNA expression of clock genes and inflammation-related genes disrupted by reversed feeding(Hindawi, 2022) Fedchenko, T.; Izmailova, O.; Shynkevych, V.; Shlykova, O.; Kaidashev, I.; Федченко, Тетяна Юріївна; Ізмайлова, Ольга Віталіївна; Шинкевич, Вікторія Ігорівна; Шликова, Оксана Анатоліївна; Кайдашев, Ігор ПетровичThe master clock, which is located in the suprachiasmatic nucleus (SCN), harmonizes clock genes present in the liver to synchronize life rhythms and bioactivity with the surrounding environment. The reversed feeding disrupts the expression of clock genes in the liver. Recently, a novel role of PPAR-γ as a regulator in correlating circadian rhythm and metabolism was demonstrated. This study examined the influence of PPAR-γ agonist pioglitazone (PG) on the mRNA expression profile of principle clock genes and inflammation-related genes in the mouse liver disrupted by reverse feeding. Methods. Mice were randomly assigned to daytime-feeding and nighttime-feeding groups. Mice in daytime-feeding groups received food from 7 AM to 7 PM, and mice in nighttime-feeding groups received food from 7 PM to 7 AM. PG was administered in the dose of 20 mg/kg per os as aqueous suspension 40 μl at 7 AM or 7 PM. Each group consisted of 12 animals. On day 8 of the feeding intervention, mice were sacrificed by cervical dislocation at noon (05 hours after light onset (HALO)) and midnight (HALO 17). Liver expressions of Bmal1, Clock, Rev-erb alpha, Cry1, Cry2, Per1, Per2, Cxcl5, Nrf2, and Ppar-γ were determined by quantitative reverse transcription PCR. Liver expression of PPAR-γ, pNF-κB, and IL-6 was determined by Western blotting. Glucose, ceruloplasmin, total cholesterol, triglyceride concentrations, and ALT and AST activities were measured in sera by photometric methods. The null hypothesis tested was that PG and the time of its administration have no influence on the clock gene expression impaired by reverse feeding. Results. Administration of PG at 7 AM to nighttime-feeding mice did not reveal any influence on the expression of the clock or inflammation-related genes either at midnight or at noon. In the daytime-feeding group, PG intake at 7 PM led to an increase in Per2 and Rev-erb alpha mRNA at noon, an increase in Ppar-γ mRNA at midnight, and a decrease in Nfκb (p65) mRNA at noon. In general, PG administration at 7 PM slightly normalized the impaired expression of clock genes and increased anti-inflammatory potency impaired by reversed feeding. This pattern was supported by biochemical substrate levels—glucose, total cholesterol, ALT, and AST activities. The decrease in NF-κB led to the inhibition of serum ceruloplasmin levels as well as IL-6 in liver tissue. According to our data, PG intake at 7 PM exerts strong normalization of clock gene expression with a further increase in Nrf2 and, especially, Ppar-γ and PPAR-γ expression with inhibition of Nfκb and pNF-κB expression in daytime-feeding mice. These expression changes resulted in decreased hyperglycemia, hypercholesterolemia, ALT, and AST activities. Thus, PG had a potent chronopharmacological effect when administered at 7 PM to daytime-feeding mice. Conclusions. Our study indicates that reversed feeding induced the disruption of mouse liver circadian expression pattern of clock genes accompanied by increasing Nfκb and pNF-κB and IL-6 expression and decreasing Nrf2 and PPAR-γ. Administration of PG restored the clock gene expression profile and decreased Nfκb, pNF-κB, and IL-6, as well as increased Nrf2, Ppar-γ, and PPAR-γ expression. PG intake at 7 PM was more effective than at 7 AM in reversed feeding mice.Документ PPARG agonist pioglitazone influences diurnal kidney medulla mRNA expression of core clock, inflammation- , and metabolism- related genes disrupted by reverse feeding in mice(Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society, 2022-11) Izmailova, O.; Kabaliei, A.; Shynkevych, V.; Shlykova, O.; Kaidashev, I.; Ізмайлова, Ольга Віталіївна; Кабалєй, Аліна Вікторівна; Шинкевич, Вікторія Ігорівна; Шликова, Оксана Анатоліївна; Кайдашев, Ігор ПетровичThis study examined the influence of PPARG activation by pioglitazone (PG) on the mRNA of core clock, inflammation‐ and metabolism‐related genes in the mouse kidney medulla as well as urinary sodium/potassium excretion rhythms disrupted by reverse feeding. Mice were assigned to daytime feeding and nighttime feeding groups. PG 20 mg/kg was administered at 7 am or 7 pm. On day 8 of the feeding intervention, mice were killed at noon and midnight. Kidney medulla expression of Arntl, Clock, Nr1d1, Cry1, Cry2, Per1, Per2, Nfe2l2, Pparg, and Scnn1g was determined by qRT PCR. We measured urinary K+, Na+, urine volume, food, and H2O intake. The reverse feeding uncoupled the peripheral clock gene rhythm in mouse kidney tissues. It was accompanied by a decreased expression of Nfe2l2 and Pparg as well as an increased expression of Rela and Scnn1g. These changes in gene expressions concurred with an increase in urinary Na+, K+, water excretion, microcirculation disorders, and cell loss, especially in distal tubules. PG induced the restoration of diurnal core clock gene expression as well as Nfe2l2, Pparg, Scnn1g mRNA, and decreased Rela expressions, stimulating Na+ reabsorption and inhibiting K+ excretion. PG intake at 7 pm was more effective than at 7 am.Документ PPARG stimulation restored lung mRNA expression of core clock, inflammation- and metabolism-related genes disrupted by reversed feeding in male mice(John Wiley & Sons Inc., 2023-09-13) Shlykova, O.; Izmailova, O.; Kabaliei, A.; Palchyk, V.; Shynkevych, V.; Kaidashev, I.; Шликова, Оксана Анатоліївна; Ізмайлова, Ольга Віталіївна; Кабалєй, Аліна Вікторівна; Пальчик, Віталіна Вікторівна; Шинкевич, Вікторія Ігорівна; Кайдашев, Ігор ПетровичThe circadian rhythm system regulates lung function as well as local and systemic inflammations. The alteration of this rhythm might be induced by a change in the eating rhythm. Peroxisome proliferator-activated receptor gamma (PPARG) is a key molecule involved in circadian rhythm regulation, lung functions, and metabolic processes. We described the effect of the PPARG agonist pioglitazone (PZ) on the diurnal mRNA expression profile of core circadian clock genes (Arntl, Clock, Nr1d1, Cry1, Cry2, Per1, and Per2) and metabolism- and inflammation-related genes (Nfe2l2, Pparg, Rela, and Cxcl5) in the male murine lung disrupted by reversed feeding (RF). In mice, RF disrupted the diurnal expression pattern of core clock genes. It decreased Nfe2l2 and Pparg and increased Rela and Cxcl5 expression in lung tissue. There were elevated levels of IL-6, TNF-alpha, total cells, macrophages, and lymphocyte counts in bronchoalveolar lavage (BAL) with a significant increase in vascular congestion and cellular infiltrates in male mouse lung tissue. Administration of PZ regained the diurnal clock gene expression, increased Nfe2l2 and Pparg expression, and reduced Rela, Cxcl5 expression and IL-6, TNF-alpha, and cellularity in BAL. PZ administration at 7 p.m. was more efficient than at 7 a.m.Документ Взаємозв’язок імунопатогенезу хронічного генералізованого пародонтиту із гістологічними особливостями ясен(Вищий державний навчальний заклад України «Українська медична стоматологічна академія», 2008) Шинкевич, Вікторія Ігорівна; Шинкевич, Виктория Игоревна; Shynkevych, V.Дослідження та краще розуміння патогенетичних ланок при хронічному генералізованому пародонтиті (ХГП) є актуальною проблемою. Імунні клітини при ХГП входять до складу інфільтратів у locus morbi, беруть участь в патогенезі. Однак, їх вплив на морфологічні особливості пародонту визначають не лише механізми запалення і тканинної деградації. Імуноцити виконують роль у підтриманні нормальної будови, росту, диференціювання, регенерації тканин. Отже, в роботі проаналізовано можливі взаємозв’язки імунних процесів та гістологічних особливостей ясен при ХГП. Результати показали нерівнозначність локальних імунних процесів у відповідності до І-ІІІ ступенів тяжкості ХГП. Перебудова тканин внаслідок імунних процесів, на фоні прогресування захворювання вкладається у поняття ремоделювання пародонту; Углубленное исследование патогенетических звеньев при хроническом генерализованном пародонтите (ХГП) является актуальной проблемой стоматологии. Иммунные клетки при ХГП представлены в ифильтратах locus morbi, принимают участие в патогенезе заболевания. Тем не менее, их влияние на морфологические особенности тканей пародонта не ограничивается механизмами воспаления. Иммунные клетки играют роль в поддержании нормальной структуры, роста, дифференцировки, регенерации тканей. Поэтому, задача состояла в анализе возможных взаимосвязей иммунных процессов и гистологических особенностей десны при ХГП. Результаты показали зависимость локальных иммунных процессов соответственно І-ІІІ степеням тяжести заболевания. Перестройка тканей вследствие имунных процессов, на фоне прогрессирования ХГП соответствует понятию ремоделирование пародонта; Thorough study of pathogenetic chains under chronic generalized parodontitis (CGP) is one of the urgent dental problems. Immune cells under CGP are represented in locus morbi infiltrates and participate in pathogenesis of the disease. Nevertheless their influence upon the morphological characteristics of peridontal tissues is not cut down with inflammatory mechanisms. Immune cells play an important role in maintenance of normal structure, differentiation, regeneration of tissues. Therefore, the task consisted in the analysis of possible interrelations of immune processes and histological peculiarities of gums under CGP. The results have shown the interdependence between local immune processes and I-III degrees of disease severity accordingly. Tissue changes resulted from the immune processes against CGP progressing correspond to the conception of periodontium remodeling.