Перегляд за Автор "Skikevych, Margarita"

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    A case report of a rare course of secondary chronic odontogenic osteomyelitis of the upper jaw in a young patient
    (Frontiers of Oral and Maxillofacial Medicine, 2023-03-15) Skrypnyk, Maksym; Skikevych, Margarita; Petrushanko, Tatiana; Скрипник, Максим Ігорович; Скікевич, Маргарита Георгіївна; Петрушанко, Тетяна Олексіївна
    Chronic jaw osteomyelitis is an inflammatory disease of the entire bone which results in jaw destruction and sequestrum formation, usually it`s more common in the lower jaw. Only a few clinical cases have documented the development of chronic odontogenic osteomyelitis on the upper jaw that is why the clinical course of the disease is variable and not typical in different patients. A quite rare variant of chronic maxilla osteomyelitis manifestation in a healthy young individual that was undiagnosed for a long time is presented in this case report.
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    Документ
    Interleukin-10 induces TNF-driven apoptosis and ROS production in salivary gland cancer cells
    (Heliyon, 2024) Skrypnyk, Maksym; Yatsenko, Tetiana; Riabets, Oleksandra; Salama, Yousef; Skikevych, Margarita; Osada, Taro; Tobita, Morikuni; Takahashi, Satoshi; Hattori, Koichi; Heissig, Beate; Скрипник, Максим Ігорович; Скікевич, Маргарита Георгіївна
    Treatment resistance after chemo-/immunotherapy occurs in patients with head and neck squamous cell cancers (HNSCs), including salivary gland cancers (SGCs). Interleukin-10 (IL-10), a cytokine with pro- and anti-cancer effects, has an unclear impact on HNSC/SGC cells. We show that HNSC patients exhibiting high expression of IL-10 and its receptor IL-10Rα experience have prolonged overall survival. Immunoreactive IL-10 was low in ductal cells of human SGC biopsies. Human (A253) and murine WR21-SGC cells expressed IL-10Rβ, but only A253 cells expressed IL-10 and IL-10Rα. The addition of recombinant IL-10 impaired SGC cell proliferation and induced apoptosis in vitro. N-acetylcysteine restored IL-10-induced reactive oxygen species (ROS) production but did not prevent IL-10-mediated viability loss. Mechanistically, recIL-10 delayed cell cycle progression from G0/G1 to the S phase with cyclin D downregulation and upregulation of NF-kB. IL-10 increased tumor necrosis factor-α (TNF-α) in A253 and WR21 and FasL in WR21 cells. Neutralizing antibodies against TNF-α and NF-kB inhibition restored SGC proliferation after IL-10 treatment, emphasizing the critical role of TNF-α and NF-kB in IL-10-mediated anti-tumor effects. These findings underscore the potential of IL-10 to impede SGC cell growth through apoptosis induction, unraveling potential therapeutic targets for intervention in salivary gland carcinomas.