Перегляд за Автор "Vazhnichaya, Elena"
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Документ Cytotoxicity of magnetite nanoparticles deposited in sodium chloride matrix and their functionalized analogues in erythrocytes(ELSEVIER, 2023) Lytvyn, Stanislav; Vazhnichaya, Elena; Kurapov, Yurii; Semaka, Oleksandr; Babijchuk, Lyubov; Zubov, Pavlo; Важнича, Олена МитрофанівнаThe synthesis of covered nanoparticles provides new properties to the materials for biomedical applications. This fully applies to iron oxide nanoparticles. The research aim was to study features of the magnetite nanoparticles synthesized by electron beam technology as well as to investigate their functionalization and cytotoxicity. Nanoparticle characteristics were determined by stan dard methods. Cytotoxiciy of nanoparticles was studied using erythrocyte model. It was shown that the original magnetite nanoparticles in the sodium chloride matrix can be functionalized with polyvinylpyrrolidone and ethylmethylhydroxypyridine succinate, an antioxidant. All investigated nanoparticles were non-toxic for erythrocytes at concentrations up to 100 μg Fe/ml. At 100-200 μg Fe/ml, they increased the amount of cells expressing phosphatidylserine on the outer membrane, the count of pathological forms of erythrocytes and hemolysis. These phe nomena were less pronounced if the nanosystem included the antioxidant. Therefore, magnetite nanoparticles can be obtained by electron beam technology and functionalized to form non-toxic nanosystems.Документ Effect of adamantane derivative on expression of biofilm-associated genes in methicillin-resistant Staphylococcus aureus(Elsevier B.V, 2023) Humeniuk, Nataliia; Zelena, Liubov; Vrynchanu, Nina; Ishchenko, Liudmyla; Bukhtiarova, Tetiana; Korotkij, Yurii; Vazhnichaya, Elena; Важнича, Олена МитрофанівнаIn the etiology of nosocomial infections, the leading role belongs to such microorganisms as Staphylococcus aureus. Insufficient effectiveness of antimicrobial chemotherapy and practical absence of drugs with an antibiofilm activity encourage the development of new remedies. The study presents results on antibiofilm activity of the adamantane derivative 1‐[4‐(1‐adamantyl)phenoxy]‐3‐(N‐benzyl,N‐dimethylamino)‐2‐propanol chloride (KVM‐97) against methicillin‐resistant S. aureus (MRSA) and the effect of this agent on the expression genes that provide regulatory and biosynthetic functions of biofilms. The ability of adamantane derivative to affect S. aureus biofilms was tested by microtiter dish biofilm formation assay. The effect of KVM‐97 on expression level of genes icaA, icaD, icaR, agrA, sarA, clfB, fib, fnbB, ebpS, and eno was detected by the real‐time PCR. The KVM‐97 inhibits the formation of S. aureus biofilms at 5.0 MIC by 95.1 % and at 0.5 MIC – by 22.4 %. Under the action of KVM‐97, destruction of the mature biofilms was not detected. It inhibits expression of the icaADBC operon and agrA gene, as well as stimulates a transcriptional activity of the icaR regulator. Subinhibitory concentrations of the KVM‐97 significantly inhibit the expression of clfB, fib, fnbB, ebpS, and eno, but does not change the sarA gene expression. Thus, 1‐[4‐(1‐adamantyl)phenoxy]‐3‐(N‐benzyl,N‐dimethylamino)‐2‐propanol chloride shows pronounced antibiofilm activity against MRSA at the early stages of the biofilm forming. This effect may be relating to influence on the expression of genes that regulate adhesion to the substrate and formation of S. aureus biofilms.Документ Systemic effect of the antioxidant gel in experimental dermatologic pathology(2024) Vazhnichaya, Elena; Baliuk, Olena; Sydorenko, Antonina; Важнича, Олена Митрофанівна; Балюк, Олена Євгеніївна; Сидоренко, Антоніна ГригорівнаMany dermatological diseases are accompanied by oxidative stress and natural antioxidants are used in dermatology and cosmetology. The efforts to use synthetic antioxidants for skin care and treatment are practically absent. That is why we select for this investigation new topical formulation of ethylmethylhydroxypyridine succinate (EMHPS), a synthetic antioxidant. The objective is to study the status of lipid peroxidation and antioxidant protection in the blood of animals with experimental dermatologic pathology under the influence of the EMHPS gel topical use. There were three series of experiments on 125 adults male Wistar rats with chemical depilation, acute ultraviolet skin damage and psoriatic-like dermatitis. The 5% EMHPS gel was applied to the skin in a dose of 125 mg/kg. The duration of treatment was 21 days after chemical depilation, 3 days after ultraviolet irradiation, and 6 days after the induction of imiquimod dermatitis. The animals were euthanized in different moments of the experiments and the malondialdehyde (MDA) concentration, superoxide dismutase (SOD) and catalase activities were determined in the blood. The data were statistically processed by ANOVA method with a post-hoc Tukey test.Документ The influence of pure (ligandless) magnetite nanoparticles functionalization on blood gases and electrolytes in acute blood loss(Elsevier, 2023) Vazhnichaya, Elena; Lytvyn, Stanislav; Kurapov, Yurii; Semaka, Oleksandr; Lutsenko, Ruslan; Chunikhin, Alexander; Важнича, Олена Митрофанівна; Литвин, Станіслав Єгорович; Курапов, Юрій Анатолійович; Семака, Олександр Валентинович; Луценко, Руслан Володимирович; Чуніхін Олександр ЮрійовичObjective was to compare the effect of functionalization of magnetite (Fe3O4) nanoparticles (NPs) with sodium chloride (NaCl), or its combination with ethylmethylhydroxypyrydine succinate (EMHPS) and polyvinylpyrrolidone (PVP) on blood gases and electrolytes in acute blood loss. Ligandless magnetite NPs were synthesized by the electron beam technology and functionalized by mentioned agents. Size of NPs in colloidal solutions Fe3O4@NaCl, Fe3O4@NaCl@EMHPS, Fe3O4@NaCl@PVP, Fe3O4@NaCl@EMHPS@PVP (nanosystems 1–4) was determined by dynamic light scattering. In vivo experiments were performed on 27 Wistar rats. Acute blood loss was modeled by removal 25 % circulating blood. Nanosystems 1–4 were administered to animals intaperitoneally after the blood loss with followed determination of blood gases, pH and electrolytes. In blood loss, nanosystems Fe3O4@NaCl and Fe3O4@NaCl@PVP were able to improve the state of blood gases, pH, and the ratio of sodium/potassium in the blood. So, magnetite NPs with a certain surface modification can promote oxygen transport under hypoxic conditions.Документ The influence of topical antioxidant use on hair regrowth and skin condition after chemical depilation(Brazilian Society of Dermatology, 2025) Baliuk, Olena; Akimov, Oleh; Vazhnichaya, Elena; Kostenko, Vitalii; Starchenko, Ivan; Балюк, Олена Євгеніївна; Акімов, Олег Євгенович; Важнича, Олена Митрофанівна; Костенко, Віталій Олександрович; Старченко, Іван ІвановичIntroduction: Hair restoration treatments for alopecia using existing medications are not always effective, highlighting the need for new therapeutic options. Objective: This study aimed to evaluate the effects of a topical gel containing ethyl-methyl-hydroxypyridine succinate (EMHPS) on hair regrowth and biochemical and histological skin parameters in animals following chemical depilation. Methods: Experiments were conducted on 50 adult male Wistar rats. Alopecia was induced using a commercial depilatory product containing potassium thioglycolate. A 5% EMHPS gel (125 mg/kg) was applied daily to the depilated skin. Trichoscopy, biochemical analysis, and histological examination of skin samples were performed on days 3, 9, and 21 of treatment. Results: The EMHPS gel demonstrated a tendency to accelerate hair regrowth, reduce lipid peroxidation, normalize antioxidant enzyme activity, and restore hydroxyproline and glycosaminoglycan levels in the treated skin compared to the untreated pathology control. Conclusions: The EMHPS gel primarily influences skin biochemical parameters and may be beneficial for treating forms of alopecia associated with oxidative stress.