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Документ Вплив детергенту, хімотрипсину та фагів на утворення біоплівок стафілококами(Українська медична стоматологічна академія, 2017) Семенчук, П. О.; Соколова, І. Є.; Воробєй, Є. С.; Вінніков, А. І.; Семенчук, П. А.; Соколова, И. Е.; Воробей, Е. С.; Винников, А. И.; Semenchuk, P. A.; Sokolova, I. E.; Vorobey, E. S.; Vinnikov, A. I.У статті представлено результати досліджень впливу детергенту (додецилсульфату натрію), хімотрипсину, і стафілококового фагу на утворення біоплівки культурою Staphylococcus aureus. Встановлено, що всі перелічені чинники виявляють відносно стафілококової біоплівки руйнівну дію, ступінь якої корелює з підвищенням концентрації означених речовин; В статье представлены результаты исследований влияния детергента (додецилсульфата натрия), химотрипсина, и стафилококкового фага на образование биоплёнки культурой Staphylococcus aureus. Установлено, что все перечисленные факторы проявляют относительно стафилококковой биоплёнки деструктивное действие, степень которого коррелирует с повышением концентрации указанных веществ; The aim of this work was investigation of influence of sodium dodecyl sulfate, chymotrypsin and phages on formation of biofilms by staphylococcus. Biological objects of study were able to film formation of clinical strain of Staphylococcus aureus isolated from patients with disorders of the gastrointestinal tract and bacteriophages from commercial preparation «Bacteriophage staphylococcal liquid» (JSC «Biopharma», Ukraine). To establish a dedicated clinical strain belonging to the species S. aureus were checked its physiological and biochemical properties, such as hemolytic, lipase and lecithinase activities in accordance seeding bacteria on blood and milk-yolk-salt agar, and also plasmocoagulase activity was determined using citrate rabbit plasma. Staphylococcus are capable to strike almost any organs and tissues of the human body. One of the important factors of pathogenic S. aureus is formation of biofilms, which is part of the life cycle and protect of bacteria from adverse environmental factors. The forming film staphylococcus rapidly acquire antibiotic resistance, and in microbial associations with other agents resistance is even more pronounced. The factors of internal environment such as hormones, enzymes, antibodies, endogenous antibiotics and other metabolites influence essentially on biofilmforming ability of opportunistic microorganisms. The substances whose effects on biofilm studied, chosen not by chance. Thus, there is no doubt that the process of film-forming on the mucosa of the intestine depend on digestive enzymes, especially proteases (trypsin and chymotrypsin) that can break down the protein components in the cell walls and membranes of bacteria and in matrix of biofilms. Determination of detergent effects on film-forming ability is important to develop means of disinfection. The study of bacteriophages action is need for creation of effective drugs for treatment of staphylococcal infections caused by film-forming strains. For biofilms growing 0.4 ml suspension of S. aureus, 1.5 ml nourishing broth and 0.1 ml examined substances in according concentration carried in each well of laboratory plate. The samples were incubating at 37°С during 72 h. After cultivation the medium rest removed from wells, the sediment dissolved in 2 ml physiological solution and tested the optical density at 540 nm. Definition of sodium dodecyl sulfate (SDS) influence on formation of biofilms by staphylococcus shown that optical density of bacteria in biofilms decreases with increasing concentrations of SDS. At the minimal concentration of SDS (0.05 mg/ml) the number of bacteria in the biofilm was 58.7% of control, increasing detergent concentrations (0.5 and 5 mg/ml) some more reduced biofilm density, but not so much as expected, and amounted to 52.3 and 42.2% of controls. Based on the findings, we can suggest that sodium dodecyl sulfate, as detergent, causing destructive effect on the cells of themselves staphylococcus and the elements of the matrix, so that part of bacteria fixed in the biofilm can come in free planktonic phase, and the density of the film as a result significantly reduce. In the study chymotrypsin impact on the biofilm-formation the enzyme concentrations selected so that they would be equivalent to the one that really exist in the body. The chymotrypsin concentration in healthy human intestinal is approximately equal to 0.025% of the total volume of secretions of the slim intestine. So the experimental final concentrations of enzyme in the wells of plate were 0.05, 0.025 and 0.0125 mg/ml. The optical density figures of staphylococcus suspensions in biofilms for increasing concentrations of chymotrypsin (0.0125, 0.025 and 0.05 mg/ml) were reduced after 3-day incubation with the enzyme to 63.3, 51.4 and 36.7% compared to control. Therefore destructive action of chymotrypsin relatively biofilms raise with increasing concentration of the enzyme. To study the influence of phage on the formation of staphylococcus biofilms used commercial preparation Bacteriophagum staphylococcus fluidum. The phage activity is first checked by Gracia method in their ability to form negative colonies on the medium with investigational clinical strain of S. aureus. The initial titre of bacteriophage was equal to 8,0 × 108 BFU/ml (bacteriophage forming units) and its final concentration in the wells was 4,0 × 107 BFU/ ml. The obtained experimental data had demonstrated reducing of staphylococcus biomass in biofilm almost twice after three days incubation with the bacteriophage, namely the optical density of microbial suspensions obtained from biofilm decreased to 51% compared to the control of native biofilm.Документ Вплив метаболітів стрептоміцетів на фізіологічну активність кукурудзи(Українська медична стоматологічна академія, 2017) Нікітенко, Т. Г.; Зубарева, І. М.; Жерносєкова, І. В.; Вінніков, А. І.; Никитенко, Т. Г.; Зубарева, И. М.; Жерносекова, И. В.; Винников, А. И.; Nikitenko, T. G.; Zubareva, I. M.; Zhernosekova, I. V.; Vinnikov, A. I.Мета. Дослідити умови приживаності стрептоміцету штаму Streptomyces recifensis var. lyticus П-29 в ризосфері кукурудзи сорту Аквазор. Встановити вплив стрептоміцету на морфобіохімічні ознаки рослини. Методи. Використовували біохімічні, біометричні, мікробіологічні методи. Результати. Встановлено, що максимальний показник приживаності стрептоміцету штаму S recifensis var. lyticus П-29 у ризосфері кукурудзи сорту Аквазор складав 40,2%, що супроводжувалося збільшенням ваги рослини на 18,0% та підвищенням пероксидазної активності коренів на 15,0%. Результати дослідження можуть бути використані у сільському господарстві для виготовлення високоефективних препаратів, які проявляють рістстимулюючу дію до рослин; Цель. Исследовать условия приживаемости стрептомицета штамма Streptomyces recifensis var. lyticus П-29 в ризосфере кукурузы сорта Аквазор. Установить влияние стрептомицета на морфобиохимические признаки растения. Методы. Использовали биохимические, биометрические, микробиологические методы. Результаты. Установлено, что максимальный показатель приживаемости стрептомицета штамма S. recifensis var. lyticus П-29 в ризосфере кукурузы сорта Аквазор составлял 40,2%, что сопровождалось увеличением веса растения на 18,0% и повышением пероксидазной активности корней на 15,0%. Результаты исследования могут быть использованы в сельском хозяйстве для производства высокоэффективных препаратов, которые проявляют ростстимулирующее действие на растения; To improve the growth and development of plants with the aim of increasing yields in agriculture apply plant growth stimulants and physiologically active substances. These compounds accelerate the germination of seeds, root development, flowering, fruiting, governing the rest of the plants and ensure their resistance to disease. Such biologically active substances are capable of synthesizing microorganisms based on active substances which are made of biological products. Active strains of microorganisms (which are components of the biological products) do not cause human genetic effects like chemical remedies. Replacement of hazardous chemicals biological will reduce the use of fertilizers and pesticides in agriculture that will produce clean, high quality crop products. Therefore, an important issue today remains the creation and application of biological preparations on the basis of active strains of microorganisms and their metabolites for increasing the yield of cultivated plants. The aim of this work was to investigate the conditions of survival streptomycete strain Streptomyces recifensis var. lyticus P-29 in the rhizosphere of maize varieties Equator. To determine the influence streptomycete on Morton signs of plants. The object of the study were: the stimulating effect monoporosa suspension of the strain Streptomyces recifensis var. lyticus P-29 corn varieties Equator. A study of the stimulating action of metabolites streptomycete on corn varieties Equator was performed using monoporosa suspension of strain S. recifensis var. lyticus P-29. Controlled the dynamics of the development streptomycete in the sand (K1), the development of plants in sand without streptomycete (K2) and the development of plants, as spores streptomycete in germinated corn grain. The results of research and their discussion. The survival rate streptomycetes in the rhizosphere of maize discovered in the maximum number of 40.2% on the 12th day from the start of the experiment. However, in the future, the survival rate of spores gradually decreased from 8.7% to 6.5%. In the experiment, in addition to survival streptomycete studied changes mortoni signs. If you make spores of the strain P-29 under the corn germ in the amount of 14.1 ± 0,03) • 107 CFU/ml was observed an increase in the root length average of 33% throughout the observation with the maximum length on the 19th day of the experiment. With the length of the roots increased their weight, the value of which increased by an average of 26%. Also supervised the changes that have occurred in the ground part of the plant. Thus, over the period of the experiment, the length of the coleoptile in seedlings increased by 22% and weight by 32%. In General, observed increase of weight of plants 64%. It should be noted that with the increase of biometric parameters of the plants were observed changes in enzymatic activity. So, the peroxidase activity of roots was increased because of the presence of spores in the rhizosphere streptomycete 10-15% in comparison with the control.Документ Етіологічні особливості негоспітальної пневмонії у хворих різних вікових груп(Українська медична стоматологічна академія, 2017) Декун, Т. Ю.; Соколова, І. Є.; Ювко, А. В.; Братусь, О. В.; Вінніков, А. І.; Декун, Т. Ю.; Соколова, И. Е.; Ювко, А. В.; Братусь, Е. В.; Винников, А. И.; Dekun, T. Y.; Sokolova, I. E.; Yvko, A. V.; Bratys, E. V.; Vinnikov, A. I.У роботі представлені результати мікробіологічного дослідження мокротиння 273 пацієнтів, що мали підозру на негоспітальну пневмонію, за період з січня по вересень 2016 року з метою виявлення етіологічних факторів захворювання в залежності від віку. В результаті було встановлено, що домінуючими збудниками пневмонії були Haemophilus influenzae і Haemophilus parainfluenzaе (47,31 і 33,33%,), рідше виділялись Streptococcus pneumoniae і Moraxella catarrhalis (10,75 і 8,60%). У осіб середнього (36-60 р.) та похилого віку (61-90 р.) відсоток усіх виявлених патогенів був найвищим (40,93 і 24,58% відповідно), у осіб молодого (20-35 р.) та юного (14-19 р.) віку він склав 19,51 і 6,75%, у молодших вікових групах даний параметр був найнижчим – 4,74 (8-13 р.) і 3,29 (0-7 р.) %; В работе представлены результаты микробиологического исследования мокроты 273 пациентов, что имели подозрение на внебольничную пневмонию, за период с января по сентябрь 2016 года з целью выявления этиологических факторов заболевания в зависимости от возраста. В результате было установлено, что доминирующими возбудителями пневмонии были Haemophilus influenza и Haemophilus parainfluenzaе (47,31 и 33,33%,), реже выделялись Streptococcus pneumoniae и Moraxella catarrhalis (10,75 и 8,60%). У пациентов среднего (36-60 лет) и преклонного возраста 61-90 лет) процент всех выявленных патогенов был наивысшим (40,93 и 24,58% соответственно), у пациентов молодого (20-35 лет) и юного (14-19 лет) возраста он составил 19,51 и 6,75%, в младших возрастных группах данный параметр был самым низким – 4,74 (8-13 лет) и 3,29 (0-7 лет) %; A community-acquired pneumonia (CAP) is one of the most widespread and life-threatening infectious disease. A quantity of lung inflammation incidences in 2011 was 494.3 per 100 thousands of population. A certain progress in diagnostics and treatment of CAP was reached, despite this mortality is increasing, in consequence of complications and pathogenicity modification of microorganisms, formation of antibiotic resistance and also decreased immune status of population. Considering relevance of this problem, the aim of this work was identification of CAP pathogens from sputum of patients and also analysis of their etiologic structure in patients of different ages. The samples were grown in different media for cultivation – 5% sheep blood agar, chocolate agar, Sabouraud agar and EMB agar. We have investigated the sputum samples of 273 patients with CAP, who were treated during 2016 year at the laboratory of Diagnostic center of LLP Pharmacies of medical academy of Dnipro. The bacteria were isolated in 93 (34%) of 273 clinical samples, from which the leading microorganisms were Haemophilus influenzae (47.31%), H. parainfluenzae (33.33%,), S. pneumoniae (10.75%) and M. catarrhalis (8,60% of all isolates). The monoinfections (59.62%) in the etiological structure of CAP pathogens were dominate, the associations of two or three infectious agents were found according in 29.81% and 10.52% causes. The largest number of detected CAP were observed when the concentration of bacteria H. influenza, S. pneumoniae and M. catarrhalis were ≥ 106 and ≥ 108 CFU/ml (Colonies formation units per ml), while frequency of detection rate of H. parainfluenzae was highest in low titer ≤ 104 CFU/ml. Frequency of definition of certain CAP pathogens widely varied in depends of year season. Thus, the maximum number of pneumonia caused by H. influenzae (38%) and H. parainfluenzae (27%) were detected in the spring, but the degree of S. pneumoniae detection was low (9%) at this period. In summer the number of identified pathogens decreased in all organisms, and the growth of infection began with arrival of autumn. The presented data about etiological structure of community-acquired pneumonia can be used in clinical practice and underscores the needs of further study of the role of significant and atypical pathogens in development of community-acquired pneumonia.Документ Моніторинг поширення вірусу сказу серед диких та свійських тварин Дніпропетровщини(Українська медична стоматологічна академія, 2017) Парасочка, Л. Г.; Зубарева, І. М.; Вінніков, А. І.; Зубарева, И. М.; Винников, А. И.; Parasochka, L. G.; Zubareva, I. M.; Vinnikov, A. I.Мета. Провести аналіз розповсюдження сказу серед свійських та диких тварин Дніпропетровської області. Методи. Використовували метод біопроб на білих мишах. Результати. Встановили, що найбільше поширення вірусного сказу зафіксовано в 2005-2007 рр. серед диких тварин, а у 2006 та в період з 2008 по 2013 рр. – у котів та собак. Домінуючим джерелом інвазії вірусом є лисиці (близько 34,1%). Найбільше розповсюдження вірусу виявлено у Магдалинівському районі; Цель. Провести анализ распространения бешенства среди домашних и диких животных Днепропетровской области. Методы. Использовали метод биопроб на белых мышах. Результаты. Установили, что наибольшее распространение вируса бешенства зафиксировано в 2005-2007 гг. среди диких животных, а в 2006 и в период с 2008 по 2013 гг. – у котов и собак. Доминирующим источником инвазии вирусом являются лисы (около 34,1%). Наибольшее распространение вируса обнаружено в Магдалиновском районе; Rabies is an acute infectious viral disease of the central nervous system that affects all warm-blooded wild and domestic animals and humans. The mortality rate is close to 100%. Rabies is caused by a neurotropic RNA virus, which belongs to the Lyssavirus genus of the family Rhabdoviridae. The virus is found mostly in the brain, spinal cord, saliva glands, and saliva of the affected animals. In the nature, wild animals are reservoirs of the virus and they transmit the disease usually by biting and via saliva rich in the virus. Rarely, transmission by non-salivary routes has been recorded. These include aerosol transmission to humans in the laboratory and in bat-infested caves. Most often, humans contract the disease from stray domestic animals. Animals contract the disease via wounds, when they are bitten by another animal infected with rabies. Rabies is being detected in many European countries. Rabies is also found in Africa, the Near East, many Asian countries and Central and South America. The aims of the study were:2) to determine regional specificity of the spread of the disease. The prevalence of rabies in the animal population of Ukraine during the period of 2005-2013 was investigated. In 2005-2013, a total of 1 040 cases of rabies were diagnosed in Dnipropetrovsk Region and wild animals were infected more often than domestic animals. According to the records, in 2008-2013 mostly cats and dogs were infected. It is shown that the peak incidence of rabies accounted for 2005 and 2010, respectively – 162 and 157 cases. The immunofluorescence method and the mouse inoculation test were used to diagnose the disease. Rabies was diagnosed in 18.18% of domestic and in 18.64% of wild animals. The disease was found to be spread in all regions of Dnipropetrovsk Region. The largest centers of rabies infection were determined to be in the districts of Mahdalynivka, Vasylkivka, Piatykhatky, Tsarychanka, Mezhova. The highest number of rabies cases 66 was recorded in the Mahdalynivka district. Our results indicate a very uneven distribution of rabies infections and show that during 2005-2013 rabies was registered in the entire territory of Dnipropetrovsk Region. The spread of rabies in the animal population creates a great danger to the public health. The results are a scientific basis for monitoring viral rabies and can be useful in the development of measures and to prevention the spreading of rabies. 1) to analyse the epizootic status of rabies infections in domestic and wild animals in Dnipropetrovsk RegionДокумент Частота виявлення різних генотипів вірусу гепатиту С серед хворих у м. Дніпро(Українська медична стоматологічна академія, 2017) Вергун, О. М.; Воронкова, Ольга Сергіївна; Вінніков, А. І.; Винников, А. И.; Воронкова, Ольга Сергеевна; Voronkovа, О. S.; Vinnikov, A. I.; Vergun, O. M.При обстеженні методом ПЛР біологічного матеріалу на наявність генетичних маркерів вірусу гепатиту С від осіб з підозрою на гепатит визначено позитивну відповідь у 616 випадках (53%) серед усіх позитивних результатів виявлення гепатитів. Домінуючими генотипами гепатиту С серед населення м. Дніпро є 1в і 3а (41,7% і 33,4% відповідно). Мікст-генотипи найчастіше представлені комбінацією 1в+3а (6,8%); При обследовании методом ПЦР биологического материала на наличие генетических маркеров вируса гепатита С от лиц с подозрением на гепатит определен положительный ответ в 616 случаях (53%) среди всех положительных результатов выявления гепатитов. Доминирующими генотипами гепатита С среди населения г. Днепр являются 1в и 3а (41,7% и 33,4% соответственно). Микст генотипы чаще всего представлены комбинацией 1в+3а (6,8%); Viral hepatitis with parenteral mechanism of transmission of pathogens, one of which is hepatitis C, are an important problem of modern medical science and health practitioners around the world. This is due to the widespread, high morbidity, expressed polymorphism of clinical manifestations, the multiplicity of ways and factors of transmission and extremely adverse effects, which can cause hepatitis – forming the entire spectrum of chronic liver disease, including cirrhosis and hepatocellular carcinoma. In recent years a marked trend towards an increased incidence of chronic disease was fixed. According to data of WHO experts chronic hepatitis C affected about 150 mln. people and 350 thousand die each year as a result of liver disease hepatitis C virus incidence and mortality due to hepatitis C is increasing progressively in the world and, according to experts, will double till 2020. For the degree of negative impact on human health and the extent of the incidence of viral hepatitis dominant place in the structure of infectious diseases parallel with influenza and acute infectious diseases of the upper respiratory tract. The aim of research was to determine the incidence of different genotypes of HCV among the population of Dnipro city. The research was made at the laboratory «Synevo» in Dnipro city. The presence of hepatitis B virus markers in obtained blood samples from individuals (n=2510), which applied to the laboratory by a doctor direction or at own will with suspected hepatitis. The material for the research was the samples of venous blood of 616 patients with manifestation of chronic hepatitis C. All patients were directed to screening for hepatitis C in clinical indications. The age of patients ranged from 21 to 57 years. The study was made by polymerase chain reaction (PCR) in real time using a kit «AmplySens® HCV-genotype-FL» (LLC «YnterLabServys», RF), which can detect a number of subtypes of virus. Extraction RNA from clinical material carried by a kit of «RIBO-sorb» (OOO «YnterLabServys», RF) according to the instructions. From total contingent of the patients (n=2510) obtained during the second half of 2016 presence of viral hepatitis was confirmed in 1163 persons (46.3%). Of these, for 616 people (53%) the chronic hepatitis C was confirmed. A study of 616 samples of plasma often met genotypes 1b – in 257 (41.7%) cases and 3a – in 206 (33.4%) cases. Genotype 1a and 2 were confirmed in 18 cases (2.9%) and 43 (7%) relatively. The genotype 4 found in 6 people (1%), 5 and 6 genotypes did not register at all. Among mixed genotypes largest number of cases – 42 fell to the lot mixed 3a+1c (6.8%) and 1a+1c, which is registered in 38 cases (6.2%), and mixed 1b+2 found only 6 people (1%). Information about genotype is very important because it allows you to predict the response to treatment duration and dose chosen to recommend the drug. There are at least 6 HCV genotypes and many subtypes that have different sensitivity to interferon. Hepatitis C is very difficult to treat compared with hepatitis B. From the literature it is known that genotype 1b better than others to treatment with modern drugs. In contrast, genotype 1b, a positive result from treatment known for hepatitis C caused by genotypes 2 and 3 is 80%. Conclusions: study of samples on the basis of «Synevo» laboratory in Dnipro city of biological material by PCR for the presence of genetic markers identified HCV positive response in 616 cases (53%) of all positive results for hepatitis research; dominant genotypes of hepatitis C among population of Dnipro city were 1c and 3a (41.7% and 33.4% respectively). Mixed genotypes often presented combination 3a+1c (6.8%). Prospects for further research: the spread of viral hepatitis is a significant medical and social problem for Ukraine, especially for hepatitis C and important area of research is the establishment of infections virus genotypes, allowing correct therapeutic scheme for each individual patient. Research genotypes of HCV to determine prognosis and options research its distribution among the population, which in the future will develop highly effective preventive measures.