Перегляд за Автор "Yanko, N."

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  • Ескіз недоступний
    Документ
    Applications of saliva in diagnostic of diseases - A comprehensive review
    (Вищий державний навчальний заклад України «Українська медична стоматологічна академія», 2015) Kiani, M.; Yanko, N.; Pankevich, A.; Панькевич, Артур Іванович
    The current development of diagnostic biomarkers in salivary diagnostics has led to the development of robust diagnostic tools for dentists to use in making clinical decisions and predicting treatment outcomes. An increasing number of systemic diseases and conditions have been shown to be reflected diagnostically in saliva. Along with these developments are technology advancements that have overcome barriers to the widespread implementation of salivary diagnostics. These barriers include technological problems related to achieving high sensitivity, high specificity, miniaturization, high throughput, automation, portability, low cost, high functionality and speed; overcoming them has enabled researchers to detect and measure multiple disease markers. These challenges have largely been met as a result of careful studies of salivary gland physiology, development of sensitive amplification methods (ELISA, RT-PCR), and education of the scientific community in the methodology for obtaining and dealing with salivary samples. Such miniaturized saliva based diagnostic technologies as OFNASET will enable the use of minute amounts of bodily fluids to yield critical patient information that reflects health and disease status. These technologies will allow clinicians to achieve real-time and simultaneous assessment of multiple diseases. Diagnostic molecular targets in saliva are the salivary proteome and the salivary transcriptome. Salivary analysis can be done for the diagnosis of the oral, infectious, systemic, autoimmune, and hereditary diseases, malignancy, monitoring of levels of hormones, detection of drugs, bone turnover marker in saliva, forensic evidence, genetic disorders, and occupational and environmental medicine. The ability to monitor health status, disease onset and progression, and treatment out come through non-invasive means is a highly desirable goal in health care promotion and delivery. An initiative catalyzed by the National Institute of Dental and Craniofacial Research (NIDCR) has created a roadmap to achieve this goal through the use of oral fluids as the diagnostic medium to scrutinize the health and disease status. The recent advances in oral fluid biomarker diagnostics has been fuelled by novel molecular approaches (e.g. proteomics, transcriptomics and genomics) and metagenomic analyses that have broadened the discovery of microbial pathogens associated with systemic and oral diseases. Similarly, these experimental approaches have been successfully used in the diagnosis of non-infectious systemic and oral conditions (e.g., cancers, autoimmune diseases, renal disease and diabetes). Diagnostic kits for S. mutans and Lactobacillus counting and salivary buffering capacity widely use in dental practice. The p53 antibodies and salivary defensin-1 level can be detected in the saliva of patients diagnosed with oral squamous cell carcinoma. Such biomarkers as Matrix metalloproteinase-8, Matrix metalloproteinase-9, Interleukin-1β (IL-1β), Interleukin- 6 (IL-6) alone and in combination, S100 proteins, Lactoferrin, Macrophage inflammatory protein-1α, 8-hydroxy- deoxyguanosine, and periodontopatogenic bacteria were successfully implementated in periodontal disease practice due to ELISA and PCR. The future of this field will depend on further validation of disease (and stage) specific biomarkers and their incorporation into state-of-the-art, multiplex assays that are versatile, quantitative, reliable, sensitive, specific, rapid, robust, and cost effective for broad implementation in diagnostic programs. Возможность контролировать состояние здоровья, начало, прогрессирование, и лечение за- болеваний с помощью неинвазивных методов является важной задачей в продвижении и оказании меди- цинской помощи. В этой статье представлены биологические и биохимические маркеры различных заболе- ваний в слюне и научно обосновано такое её использование. Технологии, работающие со слюной, прошли путь от ИФА и ПЦР до такого миниатюрного автоматизированного портативного теста как OFNASET c высокой пропускной способностью и сравнительно низкой стоимостью. Можливість контролювати стан здоров’я, початок та прогресування, а також лікування захво- рювання через неінвазивні методи є дуже важливим завданням у просуванні і наданні медичних послуг. У цій статті представлені біологічні та біохімічні маркери різних захворювань у слині та науково обґрунтоване таке її використання. Технології, що працюють зі слиною, пройшли шлях від ІФА та ПЦР до такого мініатюрного автоматизованого портативного тесту як OFNASET із високою пропускною здатністю та порівняно низькою вартістю.
  • Ескіз недоступний
    Документ
    Problems of diagnostic of the state of pulp and pulpal diagnoses in children
    (Aluna publishing, 2024-11-06) Kaskova, L.; Yanko, N.; Vashchenko, I.; Khmil, O.; Каськова, Людмила Федорівна; Янко, Наталія Валентинівна; Ващенко, Ірина Юріївна; Хміль, Олена Всеволодівна
    The aim: to analyze diagnostic methods of the pulp status in children and align pulpal diagnoses with international and Ukrainian classifications. Material and methods: The authors searched for articles via Google using keywords such as «pulpitis classification», «pulp diagnostic in children», «reversible pulpitis», «irreversible pulpitis», «pulp necrosis» combined with «primary teeth», «permanent immature teeth». Also evidence of primary data was evaluated by levels of evidence statements and grades of recommendations for questions relating to diagnosis. Results: Level of evidence of primary data about methods of pulpal state diagnosis varies from 2 to expert opinion, grade of recommendation is C-D. Sensibility tests can be used for pulp diagnosing in primary and immature permanent teeth, in conjunction with other clinical diagnostic aids. Such conditions of pulp as complete pulp necrosis, pulpless and infected root canal system, previously treated pulpitis, and previously initiated therapy could be included in the Ukrainian classification. Pulp hyperplasia only partially corresponds pulpitis chronica hypertrophica and may be associated with any form of reversible and irreversible pulpitis. In international classifications, partial pulp necrosis includes pulpitis chronica gangraenosa and pulpitis acuta purulenta from the Ukrainian variant. Conclusions: Pulpal diagnosis in children relies on observation of clinical and periapical radiographic findings. While the Ukrainian classification covers various forms of pulpitis, it could be expanded to include pulp conditions, but only some of them completely correspond diagnoses of the Ukrainian classification. Therefore, the development of new classification of the status of the pulp in children is promising and challenging task.