Перегляд за Автор "Zubareva, I. M."

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    Вплив метаболітів стрептоміцетів на фізіологічну активність кукурудзи
    (Українська медична стоматологічна академія, 2017) Нікітенко, Т. Г.; Зубарева, І. М.; Жерносєкова, І. В.; Вінніков, А. І.; Никитенко, Т. Г.; Зубарева, И. М.; Жерносекова, И. В.; Винников, А. И.; Nikitenko, T. G.; Zubareva, I. M.; Zhernosekova, I. V.; Vinnikov, A. I.
    Мета. Дослідити умови приживаності стрептоміцету штаму Streptomyces recifensis var. lyticus П-29 в ризосфері кукурудзи сорту Аквазор. Встановити вплив стрептоміцету на морфобіохімічні ознаки рослини. Методи. Використовували біохімічні, біометричні, мікробіологічні методи. Результати. Встановлено, що максимальний показник приживаності стрептоміцету штаму S recifensis var. lyticus П-29 у ризосфері кукурудзи сорту Аквазор складав 40,2%, що супроводжувалося збільшенням ваги рослини на 18,0% та підвищенням пероксидазної активності коренів на 15,0%. Результати дослідження можуть бути використані у сільському господарстві для виготовлення високоефективних препаратів, які проявляють рістстимулюючу дію до рослин; Цель. Исследовать условия приживаемости стрептомицета штамма Streptomyces recifensis var. lyticus П-29 в ризосфере кукурузы сорта Аквазор. Установить влияние стрептомицета на морфобиохимические признаки растения. Методы. Использовали биохимические, биометрические, микробиологические методы. Результаты. Установлено, что максимальный показатель приживаемости стрептомицета штамма S. recifensis var. lyticus П-29 в ризосфере кукурузы сорта Аквазор составлял 40,2%, что сопровождалось увеличением веса растения на 18,0% и повышением пероксидазной активности корней на 15,0%. Результаты исследования могут быть использованы в сельском хозяйстве для производства высокоэффективных препаратов, которые проявляют ростстимулирующее действие на растения; To improve the growth and development of plants with the aim of increasing yields in agriculture apply plant growth stimulants and physiologically active substances. These compounds accelerate the germination of seeds, root development, flowering, fruiting, governing the rest of the plants and ensure their resistance to disease. Such biologically active substances are capable of synthesizing microorganisms based on active substances which are made of biological products. Active strains of microorganisms (which are components of the biological products) do not cause human genetic effects like chemical remedies. Replacement of hazardous chemicals biological will reduce the use of fertilizers and pesticides in agriculture that will produce clean, high quality crop products. Therefore, an important issue today remains the creation and application of biological preparations on the basis of active strains of microorganisms and their metabolites for increasing the yield of cultivated plants. The aim of this work was to investigate the conditions of survival streptomycete strain Streptomyces recifensis var. lyticus P-29 in the rhizosphere of maize varieties Equator. To determine the influence streptomycete on Morton signs of plants. The object of the study were: the stimulating effect monoporosa suspension of the strain Streptomyces recifensis var. lyticus P-29 corn varieties Equator. A study of the stimulating action of metabolites streptomycete on corn varieties Equator was performed using monoporosa suspension of strain S. recifensis var. lyticus P-29. Controlled the dynamics of the development streptomycete in the sand (K1), the development of plants in sand without streptomycete (K2) and the development of plants, as spores streptomycete in germinated corn grain. The results of research and their discussion. The survival rate streptomycetes in the rhizosphere of maize discovered in the maximum number of 40.2% on the 12th day from the start of the experiment. However, in the future, the survival rate of spores gradually decreased from 8.7% to 6.5%. In the experiment, in addition to survival streptomycete studied changes mortoni signs. If you make spores of the strain P-29 under the corn germ in the amount of 14.1 ± 0,03) • 107 CFU/ml was observed an increase in the root length average of 33% throughout the observation with the maximum length on the 19th day of the experiment. With the length of the roots increased their weight, the value of which increased by an average of 26%. Also supervised the changes that have occurred in the ground part of the plant. Thus, over the period of the experiment, the length of the coleoptile in seedlings increased by 22% and weight by 32%. In General, observed increase of weight of plants 64%. It should be noted that with the increase of biometric parameters of the plants were observed changes in enzymatic activity. So, the peroxidase activity of roots was increased because of the presence of spores in the rhizosphere streptomycete 10-15% in comparison with the control.
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    Моніторинг поширення вірусу сказу серед диких та свійських тварин Дніпропетровщини
    (Українська медична стоматологічна академія, 2017) Парасочка, Л. Г.; Зубарева, І. М.; Вінніков, А. І.; Зубарева, И. М.; Винников, А. И.; Parasochka, L. G.; Zubareva, I. M.; Vinnikov, A. I.
    Мета. Провести аналіз розповсюдження сказу серед свійських та диких тварин Дніпропетровської області. Методи. Використовували метод біопроб на білих мишах. Результати. Встановили, що найбільше поширення вірусного сказу зафіксовано в 2005-2007 рр. серед диких тварин, а у 2006 та в період з 2008 по 2013 рр. – у котів та собак. Домінуючим джерелом інвазії вірусом є лисиці (близько 34,1%). Найбільше розповсюдження вірусу виявлено у Магдалинівському районі; Цель. Провести анализ распространения бешенства среди домашних и диких животных Днепропетровской области. Методы. Использовали метод биопроб на белых мышах. Результаты. Установили, что наибольшее распространение вируса бешенства зафиксировано в 2005-2007 гг. среди диких животных, а в 2006 и в период с 2008 по 2013 гг. – у котов и собак. Доминирующим источником инвазии вирусом являются лисы (около 34,1%). Наибольшее распространение вируса обнаружено в Магдалиновском районе; Rabies is an acute infectious viral disease of the central nervous system that affects all warm-blooded wild and domestic animals and humans. The mortality rate is close to 100%. Rabies is caused by a neurotropic RNA virus, which belongs to the Lyssavirus genus of the family Rhabdoviridae. The virus is found mostly in the brain, spinal cord, saliva glands, and saliva of the affected animals. In the nature, wild animals are reservoirs of the virus and they transmit the disease usually by biting and via saliva rich in the virus. Rarely, transmission by non-salivary routes has been recorded. These include aerosol transmission to humans in the laboratory and in bat-infested caves. Most often, humans contract the disease from stray domestic animals. Animals contract the disease via wounds, when they are bitten by another animal infected with rabies. Rabies is being detected in many European countries. Rabies is also found in Africa, the Near East, many Asian countries and Central and South America. The aims of the study were:2) to determine regional specificity of the spread of the disease. The prevalence of rabies in the animal population of Ukraine during the period of 2005-2013 was investigated. In 2005-2013, a total of 1 040 cases of rabies were diagnosed in Dnipropetrovsk Region and wild animals were infected more often than domestic animals. According to the records, in 2008-2013 mostly cats and dogs were infected. It is shown that the peak incidence of rabies accounted for 2005 and 2010, respectively – 162 and 157 cases. The immunofluorescence method and the mouse inoculation test were used to diagnose the disease. Rabies was diagnosed in 18.18% of domestic and in 18.64% of wild animals. The disease was found to be spread in all regions of Dnipropetrovsk Region. The largest centers of rabies infection were determined to be in the districts of Mahdalynivka, Vasylkivka, Piatykhatky, Tsarychanka, Mezhova. The highest number of rabies cases 66 was recorded in the Mahdalynivka district. Our results indicate a very uneven distribution of rabies infections and show that during 2005-2013 rabies was registered in the entire territory of Dnipropetrovsk Region. The spread of rabies in the animal population creates a great danger to the public health. The results are a scientific basis for monitoring viral rabies and can be useful in the development of measures and to prevention the spreading of rabies. 1) to analyse the epizootic status of rabies infections in domestic and wild animals in Dnipropetrovsk Region