Пародонтопротекторное действие геля «Симбітер» при экспериментальном гингивите у крыс

Abstract

У крыс с протаминовым гингивитом в деснах повышается уровень маркеров воспаления – МДА и эластазы, активность уреазы (маркера микробного обсеменения), но снижается активность лізоцима (показатель неспецифического иммунитета) и антиоксидантного фермента каталазы. Аппликации геля с симбиотиком «Симбытер» дозозависимо снижают вышеуказанные показатели, что свидетельствует о лечебно-профилактическое действие этого пробиотического препарата; У щурів з протаміновим гінгівітом в яснах підвищується рівень маркерів запалення – МДА і еластази, активність уреази (маркера мікробного обсіменіння), але знижується активність лізоцима (показник неспецифічного імунітету) і антиоксидантного фермента каталази. Аплікації гелю з симбіотиком «Симбітером» дозозалежно знижують вищевказані показники, що свідчить про лікувально-профілактичну дію цього пробіотичного препарата; Development of focal demineralization, dental caries and periodontal disease, many authors consider as a phenomenon violation of the ecological situation in the oral cavity and the accumulation of plaque on the surface of the enamel in the area of fixing braces and other orthodontic devices. Thus on the background of significant growth of pathogenic and opportunistic microorganisms concentration of the normal flora is reduced. Therefore, the development and use in the treatment of inflammatory parodontal diseases funds, contributing to the restoration of normal microflora of an oral cavity and, in particular, periodontal tissues, is considered as a necessary condition for increasing the efficiency of treatment. One of the promising aims in this directions is the use of biological preparations , which are the representatives of the normal flora of the mouth. «Симбiтер» is a symbiotics containing 4 friendly type of probiotic bacteria (lactobacilli, proprionobacteria, bifidobacteria and acid bacteria) and having high therapeutic and prophylactic efficiency. We have proposed the formulation of gel «Симбiтер», which allows local application of the drug on the different tissues of the oral cavity. The purpose of this study was to determine the therapeutic and preventive action of different concentrations of the gel «Симбітер» in experimental gingivitis on rats. Subject and methods of research. In work was used drug «Симбітер» (acidophilic, concentrated), manufactured by « Prolisok» (Ukraine). This drug is prepared gel 3 % solution of sodium carboxymethyl cellulose, sodium salt concentration «Симбітер» 2 %, 4 % and 8 %. Experimental gingivitis reproduced with the help of a two-day applications on the gum gel Protamine sulfate on 3 % solution of sodium carboxymethyl cellulose with the concentration of Protamine 100 mg/ml Experiments were conducted on 30 rats of Wistar line (female, 11 months, live weight 245 minus 10 g), distributed into 5 equal groups: the 1st norm (intact), the 2nd experimental gingivitis: gel with Protamine sulfate on gum and cheek hour before feeding for two consecutive days; 3- rd – Gingivitis + from the first day through 30 minutes after application of the gel with the Protamine applique gel with «Симбiтер» 2 % daily for 10 days; 4 th – similarly to 3-rd group, but gel «Симбiтер» 4 % and 5-th – similar to the 3 –rd group, but gel «Симбiтер» 8 %. In the homogenate of the gums (20 mg/ml 0,05M Tris-HCl buffer, pH 7. 5) the level of inflammatory markers was determined: elastase activity and content of malondialdehyde, indicator of microbial contamination – urease activity, the rate of nonspecific immunity – lysozyme, the activity of the antioxidant enzyme catalase. Ratio of relative activity of urease and lysozyme expected degree of dysbiosis by Levytsky, according to correlation of catalase activity and concentration of MDA antioxidant – prooxidant index API was found. In addition, in the homogenate of gums the content of hyaluronic acid was determined by nephelometric method by Clement. In rats with protamine gingivitis the level of inflammatory markers in gums was increased- MDA and elastase, urease activity( as a marker of microbial), but activity of lisocime ( measure of nonspecific immunity) and antioxidant enzyme catalase was reduced. Application of the gel with the «Симбітер» dose-dependence reduces the indicators mentioned earlier, that indicates a therapeutic and preventive action of the probiotic «Симбітер».