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Документ Morphological aspects of the guinea pig submandibular salivary gland structure(Riga Stradins University Student Union, 2022) Maryniak, D.; Zakaznykova, I.; Hryn, V.; Bilash, V. ; Мариняк, Дар'я Костянтинівна; Заказникова, Ілона Володимирівна; Гринь, Володимир Григорович; Білаш, Валентина ПавлівнаДокумент Morphology of catgut implant destruction in the peritoneal cavity of male white rats(Brazilian Society of Anatomy, 2024) Hryn, V.; Kostylenko, Yu.; Maksymenko, O.; Svintsytska, N.; Bilash, V.; Tykhonova, O.; Tarasenko, Ya.; Гринь, Володимир Григорович; Костиленко, Юрій Петрович; Максименко, Олександр Сергійович; Свінцицька, Наталія Леонідівна; Білаш, Валентина Павлівна; Тихонова, Олеся Олександрівна; Тарасенко, Яна АльбертівнаIntroduction: aseptic peritonitis it is reaction of the local immune system aimed at rejection of a foreign body, which, having antigenic properties, does not (unlike a pathogen) counteract the immune system. The suture materials, namely catgut thread, used in intracavitary surgical operations possess the xenogenic properties and can use for antigenic stimulation of the immune system of the peritoneal cavity. The purpose is to study the destructive changes in the catgut implant in the peritoneal cavity of albino male rats in experimental modeling of aseptic inflammation of the peritoneum. Methods: the study involved 15 white mature male rats, weighing 286,13±6,26 g. Catgut implants, standardized in shape and size, were made of 2/0 catgut thread of 0.3 mm thick and 17 cm long, which was randomly rolled into compact balls with an area of 1 cm2, after which they were engrafted into the peritoneal cavity of rats. Morphological analysis of the findings of the study was carried out using the traditional anatomical and histological methods for obtaining serial paraffin slices of 4 µm thick (Microm HM 325), which were stained with hematoxylin-eosin. Results: the findings of our study showed that the greater omentum and epididymal omentums are the acceptors of the xenogenic implant. In all cases, without exception, the catgut implant was fixed on the marginal zone of one or another omentum. Starting from day 3 of the experiment, a clearly pronounced response of the local immune system to the catgut implant was noted. Morphologically, it was visualized by the invasion of the blood vessels into implant and the appearance of continuous concentrated layer of immunocompetent cells, which were mainly represented by lymphoid and phagocytic elements, formed around the catgut thread and in consequent penetrating into the catgut thread. Conclusion: following two weeks of staying in the tissues of one or another omentum, the catgut thread was far from complete rejection.Документ The issue of histological identification of m-cells in the peyer’s patches of albino rat small intestine(Wudawnictwo Aluna, 2022) Hryn, V.; Kostylenko, Yu.; Svintsytska, N.; Bilash, V. ; Lytovka, V.; Гринь, Володимир Григорович; Костиленко, Юрій Петрович; Свінцицька, Наталія Леонідівна; Білаш, Валентина Павлівна; Литовка, Володимир ВікторовичThe aim: Based on the above cytological signs of M-ceiis, we set the goal o f more detailed clarification o f some of their topological relationships with other enterocytes in the follicle-associated epithelium of Peyer's patches of albino rat small intestine. Materials and methods: 10 mature albino male rats weighted 200,0+20,0 g were involved into the study. Anatomical dissection with the sampling o f the sections of the small intestine containing Peyer's patches was carried out with subsequent embedment of the latter into paraffin blocks and making o f serial histological sections o f4 [im thick in the cross-section o f the small intestine, followed with hematoxylin-eosin staining. The specimens were studied and documented on the"Konus" light microscope equipped. Morphometric characteristics o f the specimen tissue structures were studied using the Sigeta X 1 mm/100 Div.x0.01 mm stage micrometer. Results: The findings of the study revealed enterocytes with phagocytic properties found in the lymphoid-associated epithelium of Peyer's patches o f the small intestine o f albino rats. Moreover, if they are clearly visualized at the light-optical level, then M-ceiis are poorly recognizable, which is consistent with a similar assessment made by other authors. Conclusions: Given this, the issue on the topology and functional purpose of M-ceiis remains uncertain to date and, thereby, the prospect o f further research is being outlined, which, in our opinion, can be successful using the method o f stereomorphological analysis. For this purpose, multilayer plastic reconstruction methods can be used for serial semi-thin sections o f Peyer's patches embedded in epoxy resin, according to the requirements of transmission electron microscopy.Документ The issue of histological identification of M-cells in the peyer’s patches of albino rat small intestine(Aluna Publishing, 2022) Hryn, V.; Kostylenko, Yu.; Svintsytska, N.; Bilash, V. ; Lytovka, V.; Гринь, Володимир Григорович; Костиленко, Юрій Петрович; Свінцицька, Наталія Леонідівна; Білаш, Валентина Павлівна; Литовка, Володимир ВікторовичThe aim: Based on the above cytological signs of M-cells, we set the goal of more detailed clarification of some of their topological relationships with other enterocytes in the follicle-associated epithelium of Peyer’s patches of albino rat small intestine. Materials and methods: 10 mature albino male rats weighted 200,0±20,0 g were involved into the study. Anatomical dissection with the sampling of the sections of the small intestine containing Peyer’s patches was carried out with subsequent embedment of the latter into paraffin blocks and making of serial histological sections of 4 μm thick in the cross-section of the small intestine, followed with hematoxylin-eosin staining. The specimens were studied and documented on the “Konus” light microscope equipped. Morphometric characteristics of the specimen tissue structures were studied using the Sigeta X 1 mm/100 Div.x0.01mm stage icrometer. Results: The findings of the study revealed enterocytes with phagocytic properties found in the lymphoid-associated epithelium of Peyer’s patches of the small intestine of albino rats. Moreover, if they are clearly visualized at the light-optical level, then M-cells are poorly recognizable, which is consistent with a similar assessment made by other authors. Conclusions: Given this, the issue on the topology and functional purpose of M-cells remains uncertain to date and, thereby, the prospect of further research is being outlined, which, in our opinion, can be successful using the method of stereomorphological analysis. For this purpose, multilayer plastic reconstruction methods can be used for serial semi-thin sections of Peyer’s patches embedded in epoxy resin, according to the requirements of transmission electron microscopy.Документ Схожість і відмінність між пейєровими бляшками тонкої кишки і молочними плямами великого чепця білих щурів(Державне некомерційне підприємство «Центр тестування професійної компетентності фахівців з вищою освітою напрямів підготовки «Медицина» і «Фармація» при Міністерстві охорони здоров’я України» (ДНП «Центр тестування»), 2025) Kostylenko, Y.; Hryn, V.; Maksymenko, O.; Bilash, V.; Svintsytska, N.; Brovarnyk, Y.; Stupak, D.; Костиленко, Юрій Петрович; Гринь, Володимир Григорович; Максименко, Олександр Сергійович; Білаш, Валентина Павлівна; Свінцицька, Наталія Леонідівна; Броварник, Ярослав Олександрович; Ступак, Дмитро СергійовичВивчено та порівняно основні принципи структурної організації пейєрових бляшок тонкої кишки з молочними плямами великого чепця білих щурів. Таким чином, молочні плями великого чепця і пейєрові бляшки тонкої кишки білих щурів-самців не відносяться до пропорційних утворень, тому що перші є дискретною формою розосередження по ходу судинно-жирових аркад великого чепця певної кількості уніфікованих за структурою лімфоїдних вузликів, тоді як пейєрові бляшки є консолідованою сукупністю подібних утворень.