Перегляд за Автор "Zachepylo, S."

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    Документ
    Application efficiency of bifidobacterium bifidum 1 and lactobacillus reuteri dsm 17938 cell-free extracts in vivo
    (2020) Книш, Оксана Василівна; Погоріла, Марина Сергіївна; Полянська, Валентина Павлівна; Зачепило, Світлана Вікторівна; Knysh, O.; Pogorila, M.; Polianska, V.; Zachepylo, S.
    Insufficient efficiency and safety of cellular probiotics encourages the search for new effective means of correction of microecological disorders. Most of the beneficial effects of probiotics are due to the biological activity of their structural components and metabolites. Recently, great hope is pinned on postbiotic products as a means of restoring the balance of intestinal microbial populations. The data obtained in this experimental study demonstrate the ability of cell-free extracts from Bifidobacterium bifidum 1 and Lactobacillus reuteri DSM 17938 cultures, cultivated in their own disintegrates supplemented with ascorbic acid, to provide anti-infection protection and correct microecological disturbances at modeling an infectious process against a background of antibiotic-induced dysbiosis in mice. The beneficial effects of cell-free extracts showed up in the acceleration of the pathogen elimination and an increase in the number of representatives of the positive intestinal microbiota. The results of the study justify the need for further clinical trials to determine the therapeutic efficacy of cell-free extracts when included in the protocols of dysbiosis treatment.
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    Документ
    Opportunities and prospects of microbiological diagnosis of ent mycosis (review)
    (2020) Bezshapochnyy, S.; Podovzhnii, O.; Polianska, V.; Zachepylo, S.; Fedorchenko, V.; Безшапочний, Сергій Борисович; Подовжній, Олександр Григорович; Полянська, Валентина Павлівна; Зачепило, Світлана Вікторівна; Федорченко, Віра Іванівна
    The review considers one of the most important problems of modern otorhinolaryngology - the diagnosis of opportunistic mycoses. Data on the species spectrum of pathogens of oppor-tunistic mycoses of the ENT organs and their epidemiology are presented, the analysis of existing methods of laboratory diag-nosis of this pathology is given. The solution to the problem of rapid and reliable identification of fungi and determination of their sensitivity to antifungals is provided by the use of semi-automatic and automatic microbiological analyzers, as well as the use of mass spectrometry. Particular attention is paid to modern molecular genetic methods, such as polymerase chain reaction, sequencing of a new generation, which in combination with classical microbiological methods, make it possible, with the highest level of reliability, to identify microorganisms by species based on decoding their genomes. It also gives the pos-sibility to assess the sensitivity of the isolated strains to chemo-therapeutic drugs using the disco-diffusion method, the method of serial dilutions, epsilometric and the use of ATB strips. The introduction into clinical practice a set of classical and modern diagnostic technologies should become the standard for the di-agnosis of fungal diseases not only upper respiratory tract and ear, but also other biotopes of the macroorganism.