Alterations of the intensity of neun-immunoreactivity reactions in the cerebellar structural components of rats under influence of the food additives complex

Abstract

To estimate the changes of the intensity of the NeuN-immunoreactivity reaction in the structural components of the cerebellum of rats under the influence of the food additives complex. We used 100 white rats with an average weight of 258.1±0.67 g. in our study. We used paraffin sections with a thickness of 3 μm for immunohistochemical studies. Rat monoclonal antibody NeuN (0.N.211) sc-71667 (Santa Cruz Biotechnology, inc.) was used to detect neurons. During the morphometric study we identified that in the course of the experiment, the average number of NeuN-positive Purkinje cells of the ganglion layer of the cerebellar cortex of rats in different experimental groups (from 1st to 5th ) in comparison with the control group of animals significantly increases at p˂0.05. Concurrently, we determined morphometrically that in the course of the experiment the average number of NeuN - positive Golgi cells of type II of the granular layer of the cerebellar cortex of rats in different experimental groups in comparison with the control group of animals also increased significantly at p˂0.05. In our experimental study, we determined reliably that NeuN- positive structures of the gray matter of the cerebellum were different, both structurally and morphometrically.