Небезпека використання трансгенної раундапостійкої сої в продуктах харчування дітей через наявність у ній неідентифікованих факторів

Abstract

Проведеними дослідженнями за допомогою біологічного тесту виявлено наявність неідентифікованого фактору в бобах трансгенної раундапостійкої сої, в жовтку і білку яєць курей та м’язовій і кістковій тканині щурів, яким згодовували таку сою, обґрунтовується стимулюванням розмноження інфузорій тетрахімена піріформіс у поживному середовищі водної витяжки сої, водному екстракті після кип’ятіння жовтка і білка яєць курей та водному екстракті (бульйоні) м’язової і кісткової тканини щурів. Це є підтвердженням, що в трансген- ній раундапостійкій сої присутній неідентифікований фактор, який викликає небезпеку використання такої сої в продуктах харчування дітей, підлітків і молодих людей; Проведенными исследованиями с помощью биологического теста выявлено наличие неидентифицированного фактора в бобах трансгенной раундапостойкой сои, в желтке и белке яиц кур и мышечной и костной ткани крыс, которым скармливали такую сою, обосновывается стимулированием размножения инфузорий тетрахимены пириформис в питательной среде водной вытяжки сои, водном экстракте после кипячения желтка и белка яиц кур и в водном экстракте (бульоне) мышечной и костной ткани крыс. Это является подтверждением, что в трансгенной раундапостойкой сои присутствует неидентифицированый фактор, который вызывает опасность использования такой сои в продуктах питания детей, подростков и молодых людей; Research purpose. Detecting the presence of unidentified factor transgenic roundup resistant soybeans by method biological tests. Object and research methods. Ciliates — Tetrahymena pyriformis, after boiling water extract of soybeans and whole plant GM soybeans and non GM soy, aqueous extract of yolks and egg whites of chickens were fed GM soy (experiment) and sunflower meal (control), aqueous extract (broth) muscle and bone tissue of rats were fed GM soy (experiment) and sunflower meal (control). To obtain the aqueous extract of GM and non GM soy weighed 50 grams beans both options and covered in heat- resistant glass cups, and then added 300 ml of distilled water, heated to boiling and boil for 30 minutes. In the process of boiling occurred inactivation of urease, antitrypsin and antyhimotrypsynu and other anti-utritional bioactive substances with the transition to an aqueous solution of water-soluble fraction thermostable proteins of low molecular weight substances from non-GM soybeans and plasmid DNA fragments and other low molecular weight compounds Agrobacterium tumefaciens with GM soya beans. After boiling broth filtered through a nylon cloth and thus prepared aqueous extract of GM and non GM soy. Test each aqueous extract of soybean GM and non GM conducted a 3-time repetition. In three vials of antibiotics contributed to 1 cm3 aqueous extract roundup resistant GM soybeans and similarly in the 3-bottle — non-GM soybeans, and then added to each bottle to 0.1 cm3 3 daily culture ciliates Tetrahymena pyriformis. The following biological test based on the determined presence of unidentified factor transgenic roundup resistant soybeans in the eggs of hens based on stimulating vitality ciliates Tetrahymena pyriformis in conditions in vitro when using a nutrient medium — water extract of yolk and egg whites chickens which a part of the diet were fed transgenic roundup resistant soybeans. The presence of unidentified factor roundup resistant transgenic soy in chicken eggs is their ability to stimulate the vitality of ciliates Tetrahymena pyriformis in nutrient medium aqueous extract after boiling egg yolk and whites chicken, which in part of the diet for 2 months were fed roundup resistant transgenic soybeans compared with water extract the yolk and egg whites chicken, which is not were fed GM soy. To obtain the aqueous extract of yolk and egg whites chicken experimental and control groups performed 3 times the selection of eggs for three of the group. Smashed eggs and yolk and whites separated from the transfer of in heat-resistant glass cups, and then added for 50 ml of distilled water for one egg yolk and whites, after their thoroughly homogenized to obtain a homogeneous aqueous solution and heated to boiling, boil for 30 minutes. In the process of boiling occurred inactivation of biologically active substances with the transition to an aqueous solution of water-soluble fraction thermostable proteins, lipoproteins, phospholipids, low molecular weight compounds and fragments of ring structures of DNA and unidentified substances Agrobacterium tumefaciens from eggs of hens of the experimental group. After the boil in every glass added 2 ml of 9 % acetic acid and stirred welded whites and yolk. After filtration method of push ups through a nylon fabric obtained water extract of yolk and whites, which re-filtered through a paper filter. In the extract obtained drops pH value adjusted to 7.2-7.5 0.1 n solution of NaOH. To obtain an aqueous extract of muscle and bone tissue of experimental and control rats carried their slaughter for 3 heads from each group and carcasses, ie, muscle and bone tissue finely crushed and placed in a heat-resistant glass cups, and then added 200 ml of distilled water, heated to boiling and boil for 30 minutes. In the process of boiling occurred inactivation of biologically active substances with the transition to an aqueous solution of water- soluble fraction thermostable proteins of low molecular weight substances from the muscle and bone tissue of rats in the control group and possible fragments of DNA plasmids and unidentified compounds Agrobacterium tumefaciens tissues of animals of the experimental group. After boiling decoction filtered through a nylon cloth and thus obtained aqueous extract of muscle and bone tissue of rats experimental and control group. Test each aqueous extract of muscle and bone tissue carried from 3 rats. In three vials from antibiotics contributed to 1 cm3 of aqueous extract of muscle and bone tissue of rats research and similar in 3-bottle — the control group, and then added to every bottle to 0.1 cm3 3 daily culture ciliates Tetrahymena pyriformis. Research results and their discussion. The performed a study using a biological test revealed the presence of unidentified factor in transgenic beans resistant roundup soybean in egg yolks and egg whites chicken and muscle and bone tissue of rats were fed soy substantiated such stimulation multiplication ciliates Tetrahimena piriformis nutrient medium in aqueous extract of soy, water the extract after boiling (broth) of muscle and bone tissue of rats. Conclusions. The research results are confirmation that the transgenic soybean roundup resistant present unidentified factor that causes the danger of using such soy in food products children, adolescents and young people.