Morphology of catgut implant destruction in the peritoneal cavity of male white rats

Abstract

Introduction: aseptic peritonitis it is reaction of the local immune system aimed at rejection of a foreign body, which, having antigenic properties, does not (unlike a pathogen) counteract the immune system. The suture materials, namely catgut thread, used in intracavitary surgical operations possess the xenogenic properties and can use for antigenic stimulation of the immune system of the peritoneal cavity. The purpose is to study the destructive changes in the catgut implant in the peritoneal cavity of albino male rats in experimental modeling of aseptic inflammation of the peritoneum. Methods: the study involved 15 white mature male rats, weighing 286,13±6,26 g. Catgut implants, standardized in shape and size, were made of 2/0 catgut thread of 0.3 mm thick and 17 cm long, which was randomly rolled into compact balls with an area of 1 cm2, after which they were engrafted into the peritoneal cavity of rats. Morphological analysis of the findings of the study was carried out using the traditional anatomical and histological methods for obtaining serial paraffin slices of 4 µm thick (Microm HM 325), which were stained with hematoxylin-eosin. Results: the findings of our study showed that the greater omentum and epididymal omentums are the acceptors of the xenogenic implant. In all cases, without exception, the catgut implant was fixed on the marginal zone of one or another omentum. Starting from day 3 of the experiment, a clearly pronounced response of the local immune system to the catgut implant was noted. Morphologically, it was visualized by the invasion of the blood vessels into implant and the appearance of continuous concentrated layer of immunocompetent cells, which were mainly represented by lymphoid and phagocytic elements, formed around the catgut thread and in consequent penetrating into the catgut thread. Conclusion: following two weeks of staying in the tissues of one or another omentum, the catgut thread was far from complete rejection.