Genetic polymorphism Arg753Gln of TLR-2, Leu412Phe of TLR-3, Asp299Gly of TLR-4 in patients with influenza and influenza-associated pneumonia
Ескіз недоступний
Дата
2019
Назва журналу
Номер ISSN
Назва тому
Видавець
Aluna Publishing
Анотація
The aim of the research is to study the prevalence and to determine the prognostic significance of polymorphism ARG753GLN of the TLR-2 gene, Leu412Phe of TLR-3, Asp299Gly of TLR-4 in influenza. Materials and methods: 112 patients with influenza were examined (63 patients with uncomplicated course and 49 with influenza-associated pneumonia). The genotyping of the polymorphic site of ARG753GLN of the TLR-2 gene, Asp299Gly of the TLR-4 gene, and Leu412Phe of the TLR-3 gene was carried out by polymerase chain reaction using oligonucleotide primers. Results: It has found that the prevalence of the mutant allele 299Gly of TLR-4 in patients with uncomplicated influenza is 6.4 %, with influenza- associated pneumonia – 7.1 %, which exceeds the population control indicators by 3.8-4.3 times (1.7 %, p<0.05). Mutant allele 412Phe of TLR-3 is significantly more common in patients with influenzaassociated pneumonia (42.9%), as compared with uncomplicated influenza (24.6%, p<0.01) and healthy people (30.0%, p<0.05). The increased risk of influenza development is associated with the Asp/Gly genotype of TLR-4 (OR=4.22) and combination of mutant genotypes Leu/Phe and Phe/Phe of TLR-3 with Asp/Gly of TLR-4 and Arg/Gln of TLR-2 (OR=15.0); influenza-associated pneumonia − with genotype Phe/Phe of TLR-3 (OR=4.5).
Conclusions: It has been found out that among patients with influenza and influenza-associated pneumonia, the mutant allele 299Gly of TLR-4 and combinations of
polymorphisms Arg753Gln of TLR-2, Leu412Phe of TLR-3, Asp299Gly of TLR-4 are detected reliably more often. The frequency of the mutant allele 412Phe of TLR-3 is higher among patients with influenza-associated pneumonia. Markers of increased risk of influenza are 299Gly allele and genotype Asp/Gly of TLR-4 and the combination of mutant genotypes Leu/Phe and Phe/Phe of TLR-3 with Asp/Gly of TLR-4 and Arg/Gln of TLR-2; for influenza-associated pneumonia − allele 412Phe and genotype Phe/Phe of TLR-3.
Опис
Ключові слова
influenza, influenza-associated pneumonia, Arg753Gln polymorphism of the TLR-2 gene, Asp299Gly polymorphism of the TLR-4 gene, Leu412Phe polymorphism of the TLR-3 gene, genotype, allele
Бібліографічний опис
Genetic polymorphism Arg753Gln of TLR-2, Leu412Phe of TLR-3, Asp299Gly of TLR-4 in patients with influenza and influenza-associated pneumonia / N. O. Pryimenko, T. M. Kotelevska, T. I. Koval [et al.] // Wiadomosci lekarskie. – 2019. – Т. LXXII, Nr. 12 (1) − P. 2324−2328.