Preliminary study of the influence of maternal and neonatal NOS3 (rs1799983), IL1B (rs1143634) genes variants and their intergenic interaction on the development of hypoxic-ischemic encephalopathy in newborns in the context of treatment planning

Abstract

Aim: To determine the influence of maternal and neonatal variants of the eNOS (G894T, rs1799983) and IL1B (C3953T, rs1143634) genes and their intergenic interactions on the development of HIE in newborns. Materials and Methods: The study included a cohort of 105 newborns and their 99 mothers. Determination of variants of the genes eNOS (G894T, rs1799983) and IL1B (C3953T, rs1143634) was carried out for the patients of study groups. Results: The frequency of detection of the 894TT genotype by the eNOS gene was increased in newborns with severe asphyxia (p=0.018) and in their mothers (p=0,0057). Further analysis of intergenic interactions, performed in mother-child pairs, revealed an increased frequency of the neonatal 894GG (eNOS)/ maternal 3953С (IL-1B) genotype combination in the comparison group versus the group of newborns with HIE (p=0.007). Conclusions: The significance of the intergenic maternal combination of 894GG/3953CT genotypes for the eNOS and IL1B genes and the intergenic combination of neonatal 894GG (eNOS)/maternal 3953CT (IL-1B) genotypes in the development of HIE in newborns has been proven. Associations of maternal and neonatal 894TT genotypes for the eNOS gene with the development of severe asphyxia, bradycardia, and respiratory failure were found in newborns with HIE.