Світ медицини та біології. 2020

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https://repository.pdmu.edu.ua/handle/123456789/12286

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Перегляд Світ медицини та біології. 2020 за Автор "Akimov, O. Ye."

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    Influence of prolonged central deprivation of testosterone synthesis on production of reactive oxygen and nitrogen species and morphological structure of rat testes
    (Українська медична стоматологічна академія, 2020) Stetsuk, Ye. V.; Akimov, O. Ye.; Shepitko, V. І.; Goltsev, A. М.; Стецук, Євген Валерійович; Акімов, Олег Євгенович; Шепітько, Володимир Іванович; Гольцев, Анатолій Миколайович
    Some testicular diseases require long-term use of testosterone synthesis blockers. It is known that testosterone is a necessary component for the physiological cooperation of cell forms of the stroma and parenchyma of testes. The scientific literature contains a limited amount of data about the effect of long-term deprivation of testosterone synthesis on this cooperation. The aim of our study was to establish the effect of long-term (365 days) inhibition of hypothalamic-pituitary stimulation of spermatogenesis on morphological and biochemical parameters in rat testes. Central deprivation of testosterone synthesis leads to fibrosis with subsequent disruption of the structural organization of the convoluted seminiferous tubules, hemodynamic disorders, endothelial dysfunction, increased density of blood vessel walls and systemic congestion. A decrease in the activity of constitutive NO synthase isoforms plays a major role in the development of structural changes in the testes of rats.
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    Morphofunctional features of rat testes interstitial endocrinocytes and sustentocytes after 90 days of central testosterone synthesis deprivation
    (Українська медична стоматологічна академія, 2020) Stetsuk, Ye. V.; Akimov, O. Ye.; Shepitko, K. V.; Goltsev, A. N.; Стецук, Євген Валерійович; Стецук, Евгений Валерьевич; Акімов, Олег Євгенович; Акимов, Олег Евгеньевич; Шепітько, Костянтин Володимирович; Шепитько, Константин Владимирович; Гольцев, Анатолій Миколайович; Гольцев, Анатолий Николаевич
    With the social system development, there is a tendency to change attitudes towards family and family values. In developed European countries, there is a trend towards high sexual activity in elderly men and late creation of a family with children, which undergoes certain difficulties in connection with a decreased testosterone production in later years. The purpose of the study was to establish the microscopic organization of rat interstitial endocrinocytes and sustentocytes, to determine the sources of nitric oxide production and the intensity of oxidative stress in the testes with experimental central deprivation of testosterone synthesis with diphereline on the 90th day of the experiment. The experiments were carried out on 20 sexually mature male white rats of the Wistar line. Rats were divided into 2 groups: the control group (10) and the experimental group (10), which were injected subcutaneously with diphereline (Triptorelin embonate) at a dose of 0.3 mg / kg of the active substance for 90 days. Prolonged central deprivation of testosterone synthesis in animals leads to emergence of functional stress structural signs in population of sustentocytes and interstitial endocrinocytes, which are aimed to support testicular secretion. Central deprivation of testosterone synthesis within 90 days causes oxidative stress development owing to reactive oxygen species hyperproduction and nitrite accumulation in testicular tissue due to increased inducible NO-synthase activity;
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    Документ
    Role of nitric oxide in development of fibrotic changes in rats’ testes after 270 day central deprivation of testosterone synthesis
    (Українська медична стоматологічна академія, 2020) Stetsuk, Ye. V.; Akimov, O. Ye.; Shepitko, K. V.; Boruta, N. V.; Goltsev, A. M.; Стецук, Евгений Валерьевич; Акимов, Олег Евгеньевич; Шепитько, Константин Владимирович; Борута, Наталия Владимировна; Гольцев, Анатолий Николаевич; Стецук, Євген Валерійович; Акімов, Олег Євгенович; Шепітько, Костянтин Володимирович; Борута, Наталія Володимирівна; Гольцев, Анатолій Миколайович
    Disturbance in production of nitric oxide (NO) may lead to various changes in different organs and systems. Certain clinical situations require prolonged usage of inhibitors of testosterone synthesis. Scientific literature provides limited information regarding the influence of prolonged deprivation of testosterone synthesis on production of NO and microscopic organization of rats’ testes. Prolonged central deprivation of testosterone synthesis leads to endothelial dysfunction, development of fibrosis, decreases the nitric oxide production and shifts pro-/antioxidant balance in favor of the pro-oxidants without increase in lipid peroxidation intensity. Central deprivation of testosterone synthesis leads to fibrosis with subsequent disruption of the structural organization of the convoluted seminiferous tubules, hemodynamic disturbances, endothelial dysfunction, increased density of the vascular wall of blood vessels and systemic stasis. Decreased production of NO from constitutive isoforms of NO-synthase plays major role in development of structural changes in the interstitial tissue of testes on the 270th day of the experiment.
  • Ескіз недоступний
    Документ
    Structural organization of stromal and parenchymal components of rat testes during central deprivation of testosterone synthesis on the 180 day of the experiment
    (Українська медична стоматологічна академія, 2020) Стецук, Євген Валерійович; Стецук, Евгений Валерьевич; Stetsuk, Ye. V.; Акімов, Олег Євгенович; Акимов, Олег Евгеньевич; Akimov, O. Ye.; Шепітько, Костянтин Володимирович; Шепитько, Константин Владимирович; Shepitko, K. V.; Гольцев, Анатолій Миколайович; Гольцев, Анатолий Николаевич; Goltsev, A. M.
    Prolonged central deprivation of testosterone synthesis may lead to biochemical and morphological changes in testes. Influence of prolonged testosterone deficiency on reactive nitrogen and oxygen species production, morphological changes in interstitial endocrinocytes and sustentocytes is not yet fully understood. The number of interstitial endocrinocytes is reduced in comparison with the control group, there are interstitial spaces between the convoluted tubules with a complete absence of interstitial endocrinocytes. Interstitial endocrinocytes are reduced in size, their nuclei are heterochromic. When we studied the structural organization of rat sustentocytes from the experimental group in comparison with the control group, we found that hyperplasia of the elements of the smooth endoplasmic reticulum was observed in the cytoplasm of the sustentocytes. The number of mitochondria in the cytoplasm of the sustentocytes decreased, the electron density of the mitochondrial matrix was lowered, protein structures were present either inside the vacuoles or independently located in the cell cytoplasm. Biochemical studies revealed increased NO production from inducible NO-synthase and development of oxidative stress. Experimental central deprivation of testosterone synthesis with diphereline on the 180th day of the experiment leads to shift of NO synthesis from constitutive NO-synthases to inducible NO-synthase and intensification of oxidative stress due to increase of superoxide anion-radical production and decrease in antioxidant protection.