Світ медицини та біології. 2022

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https://repository.pdmu.edu.ua/handle/123456789/17898

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Перегляд Світ медицини та біології. 2022 за Автор "Akimov, O. Ye."

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  • Ескіз недоступний
    Документ
    Changes in nitric oxide production and development of oxidative stress in rats heart during prolonged triptorelin-induced central deprivation of luteinizing hormone synthesis
    (Poltava State Medical University, Poltava, 2022) Voroshilova, T. A.; Shepitko, V. I.; Stetsuk, Ye. V.; Akimov, O. Ye.; Puzyryov, G. S.; Ворошилова, Тетяна Анатоліївна; Шепітько, Володимир Іванович; Стецук, Євген Валерійович; Акімов, Олег Євгенович; Пузирьов, Гай Сергійович
    Central deprivation of luteinizing hormone synthesis for 365 days leads to the development of oxidative stress in rat hearts. The peak of oxidative damage to the heart under these conditions happens on the 180th day of the central deprivation of luteinizing hormone synthesis. Production of nitric oxide during central deprivation of luteinizing hormone synthesis undergoes complex changes from an initial decrease of NO-synthase-dependent nitric oxide production on the 30th and 90th day to overproduction of nitric oxide from NO-synthases on the 365th day of the experiment.
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    Документ
    Extracellular matrix of rat liver under the conditions of combining systemic inflammatory response syndrome and chronic alcohol intoxication
    (Полтавський державний медичний університет, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне Степанівна
    The aim of this study was to establish the effect of systemic inflammatory response syndrome on the background of chronic alcohol intoxication on the concentration of oxyproline, glycosaminoglycans and sialic acids in the liver of rats. The study was performed on 24 male Wistar rats. Animals were divided into 4 groups of 6 animals: I – control; II – systemic inflammatory response syndrome group; III – alcoholic hepatitis groupand IV – animals with a combination of systemic inflammatory response syndrome and chronic alcohol intoxication. The concentrations of glycosaminoglycans, heparin-heparan, keratan-dermatan and chondroitin fractions of glycosaminoglycans, free oxyproline and sialic acids were studied in the liver tissue homogenate. The combination of systemic inflammatory response syndromeand chronic alcohol intoxication leads to ncreased intensity of collagenolysis, glycoprotein catabolism and reduced intensity of breakdown of proteoglycans of connective tissue of the liver. However, it changes the ratio of individual fractions of glycosaminoglycans in the direction of reducing тtheconcentration of anti-inflammatory and increasing regenerative.
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    Документ
    Influence of transcription factor κB on remodeling of extracellular matrix of rat liver under conditions of chronic alcohol intoxication
    (Полтавський державний медичний університет, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне Степанівна
    Liver fibrosis is a common tissue reaction associated with chronic liver damage resulting from prolonged parenchymal cell damage and inflammation. The fibrogenic response is characterized by the progressive accumulation of extracellular matrix components enriched in collagen fibrils and impaired extracellular matrix remodeling. The aim of this study was to establish the effect of transcriptional factor κB on the concentration of oxyproline, glycosaminoglycans and sialic acids in the liver of rats under conditions of chronic alcohol intoxication. The study was performed on 24 male Wistar rats. The animals were divided into 4 groups of 6 animals: І – control; II – animals with inhibition of κB; ІІI – animals with chronic alcohol hepatitis and ІV – animals with κB inhibition on the background of chronic alcohol intoxication. We studied the total concentration of glycosaminoglycans, concentration of heparin-heparan, keratan-dermatan and chondroitin fractions of glycosaminoglycans, free oxyproline and sialic acids in the liver tissue homogenate. Prolonged alcohol intoxication of rats leads to remodeling of the extracellular matrix as evidenced by increased collagenolysis and catabolism of glycoconjugates of amorphous connective tissue of the liver. The use of the inhibitor of the transcription factor κB in the background of prolonged alcohol intoxication of animals reduced the concentration of GAG by 1.43 times, lowered oxyproline concentration by 1.6 times and decreased the concentration of sialic acids by 1.14 times compared to chronic alcohol intoxication group. Transcriptional factor NF-κB under conditions of chronic alcohol intoxication affects the architectural remodeling of connective tissue of rat liver by increasing degradation of collagen fibers and destruction of glycoproteins and proteoglycans of the extracellular matrix.