Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії

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5-річна динаміка особливості геометрії лівого шлуночка у хворих на ішемічну хворобу серця залежно від тактики лікування
(Українська медична стоматологічна академія, 2019) Гута, Р. Р.; Бек, Н. С.; Радченко, О. М.; Guta, R. R.; Beck, N. S.; Radchenko, O. M.
Вступ. Для пацієнта з ішемічною хворобою серця відновлення після реваскуляризації міокарда є складним процесом. Ремоделювання серця включає в себе після інфаркту міокарда, процеси гіпертрофії і дилятації, внаслідок чого порушується систолічно-діастолічна функції серця, що є додатковим чинниками ризику подій. У проспективних дослідженнях було показано зв’язок між розміром лівого шлуночка та ризиком появи серцево-судинних ускладнень. Беручи до уваги це, виникла потреба у проведенні нашого дослідження. Мета роботи: визначити поширеність типів геометрії лівого шлуночка у хворих на ішемічну хворобу серця, післяінфарктний кардіосклероз залежно від лікувальної тактики: реваскуляризація чи консервативна терапія впродовж 60 місяців. Матеріал і методи: Обстежено 101 пацієнт віком 58,6±4,2 р. після реваскуляризації міокарда (n=71) та тільки з консервативним лікуванням (n=30), в яких впродовж 5 років оцінювали динаміку ЕхоКҐ параметрів серця. За значеннями індексу маси міокарда лівого шлуночка та відносної товщини лівого шлуночка визначали типи ремоделювання. Результати та обговорення. На початку спостереження в основній групі переважали пацієнти з концентричною гіпертрофія лівого шлуночка (50,0%), а з концентричним ремоделюванням лівого шлуночка - 24,1%. Нормальна геометрія у 14,8 % та ексцентрична гіпертрофія лівого шлуночка спостерігалися у 11,1%. У групі порівняння найчастіше спостерігалась ексцентрична гіпертрофія та концентричне ремоделювання лівого шлуночка по 29,6% кожна, трохи менше 25,9% концентрична гіпертрофія і найменше було нормальної геометрії (14,8%). Через 60 місяців у пацієнтів обох груп не зустрічалася нормальна геометрія лівого шлуночка та концентричне ремоделювання. В основній групі переважав концентричний тип (80,0%), а у групі порівняння - ексцентричний тип (55,6%). Висновки. У пацієнтів з ішемічною хворобою серця, післяінфарктним кардіосклерозом у динаміці 5-річного спостереження прогресує гіпертрофія міокарда незалежно від лікування. Після реваскуляризації частіше розвивається концентрична гіпертрофія лівого шлуночка, тоді як за умов консервативного лікування - ексцентрична гіпертрофія лівого шлуночка у поєднанні з систолічною дисфункцією та дилатацією лівого передсердя. Після реваскуляризації збільшення індексу асоціювалося із зростанням не тільки маси тіла, а й змінами ліпідного профілю та діастолічною гіпертензією
A study of the time-based characteristics of phenomenology of post-stroke fatigue over the first year after stroke occurrence
(Українська медична стоматологічна академія, 2018) Delva, I. I.; Дельва, Ірина Іванівна; Дельва, Ирина Ивановна
Post-stroke fatigue (PSF) is a common medical and social problem. Aim: to analyze time-based characteristics of PSF over the first year after stroke event. Material and methods. Patients were examined through definite time slots: during hospital staying (234 cases), in 1 month (203), in 3 months (176), in 6 months (156), in 9 months (139) and in 12 months (128 cases) after stroke. PSF was measured by fatigue assessment scale (FAS) and multidimensional fatigue inventory-20. (MIF-20) We conditionally divided all PSF cases into early PSF group (presented only within the 1st month after stroke), persistent PSF (presented within the 1st post-stroke month and later) and late PSF (appeared only in 3rd month observation or later). Results. Having analyzed all PSF cases according to FAS, we found out 15 PSF cases (16,0%) were assessed as early PSF, 51 (54,2%) assessed as persistent PSF and 28 (29,8%)– as late PSF. For all timebased types of PSF domains, according to MIF-20, the similar pattern of distribution was observed: early PSF domains – from 16,3% to 20,3%, persistent PSF domains – from 54,15 to 59,8%, late PSF domains – from 23,9% to 26,0%. All domains of early PSF, according to MIF-20, were statistically more intensive than all corresponding domains of persistent PSF and late PSF, whereas intensities of all persistent PSF domains and all late PSF domains were much or less similar. Conclusions. 1. About 20% of all PSF cases are early PSF, 25% are late PSF and slightly more than half of all cases is persistent PSF. 2. Proportions of all domains of early PSF, late PSF and persistent PSF are practically similar. 3. Significant differences between severities of early PSF and persistent PSF as well as late PSF can be as indirect evidences that all timebased PSF types are quite distinctive entities.
Adaptation among foreign students in Ukraine
(Полтавський державний медичний університет, 2021) Klypachenko, I. V.; Levkov, A. A.; Клипаченко, Інна Валеріївна; Левков, Анатолій Анатолійович
The processes of globalization and internationalization are the main factors, which have made a great impact on the evolution of higher education in the last decades of the 21st century. The number of foreign higher degree students in Ukraine has grown at a significant rate in recent years, with the country offering a high quality higher education and, as a result, becoming one of the main European hubs attracting students from all over the world. In the article, the term “foreign student” is referred to any student who goes to another country in pursuit of learning. Since the emergence and popularization of the recent trend of studying abroad, the topic of adaptation of foreign students has become of the key interest among modern European scholars who have become concerned with studying the peculiarities, issues and challenges of living and learning in an unfamiliar social, cultural, and academic environment. With no doubt, studying abroad delivers new knowledge, experiences, and opportunities. However, the adaptation to a host country and a new environment that is far away from home countries, families, and friends, sometimes takes a long time. Moreover, usually, it is challenged with several difficulties including lack of motivation, discouragement, depression, stress, anxiety, psychological conditions, culture shock, loneliness, misunderstands, conflicts, communicating with peers, language incapability, understanding lectures, learning new social norms, and interacting with people from different cultural backgrounds. As a result, these stressors have a great impact on foreign students’ physical and psychological well-being and challenge their wish, motivation, opportunity and capability to participate fully in the process of the cultural and academic adaptation. Even if the universities and host society provide helpful sources and advantageous conditions to enhance the process of their adaptation, sharp differences in socio-communicative environments of the countries, as well as cultural and language differences pose several problems foreign students usually face influencing their present academic performance and future professional medical practices. In the article, the authors discuss the issues of the geographic, psychological, socio-cultural, and linguistic methods of adaptation that have an impact on foreign students’ academic performance, social integration, and career. The authors represent the findings of several studies on the topic exploring characteristic features of the adaptation of students in the higher education institutions of Ukraine. The general purpose of the article is to examine the adaptation using a theory-based explanatory approach. In this study, the adaptation is discussed as part of the social responsibilities of the universities and foreign students. On the one hand, higher education institutions must provide the necessary services and activities to help foreign students to combat the stressors they face in the process of adapting to the Ukrainian socio-cultural and educational environment. Moreover, universities need to ensure equal rights and opportunities for students’ participation in academic life, as well as apply the most effective methods of foreign language learning. On the other hand, foreign students must be motivated enough to take the advantage of the opportunities and resources provided by universities and be responsible for the success of their adaptation to the social and academic life of the host country.
Age-related changes of glycolytic activity and antioxidant capacity in the blood of alloxan diabetic rats
(Полтавський державний медичний університет, 2020) Kushnir, O. Yu.; Yaremii, I. M.; Кушнір, О. Ю.; Яремій, І. М.; Кушнир, А. Ю.; Яремий, И. М.
The increasing incidence of type 1 diabetes coupled with advances in treatment of type 1 diabetes has resulted in an unprecedented number of older adults living with and controllable type 1 diabetes. The objective of this experimental study was to assess the impact of aging on the level of basal glycaemia and activities of glucose-6-phosphate dehydrogenase [EC1.1.1.49], pyruvate kinase [EC 2.7.1.40] and glutathione reductase [EC1.6.4.2] in erythrocytes of alloxan-diabetic rats. Methods: We used 100 male Wistar rats, divided into two age groups: I group included- 2-month (adult) animals, and II group was made up of 4-month (old) animals. Diabetes was modelled by injecting the rats with 5% solution of alloxan monohydrate intraperitoneally in a dose of 170 mg/kg. Blood was taken from the tail vein to evaluate the basal glycaemia on 5-th and 47-th day after the alloxan injection. Rats were sacrificed on the 47-th day of the experiment in accordance with the regulations on ethical treatment of vertebrates. The assessment of the activity of the enzymes was carried out by standard methods. Statistical analysis was performed by using Statistica 10 StatSoft Inc. Results. The level of basal glycaemia on the fifth day of the experiment in the animals of both groups went up on average by 115% from baseline values. We founded that on 47-th day this index was higher in group of old rats by 20% than in adult rats. Pyruvate kinase activity in erythrocytes of adult and old animals with diabetes decreased by 35% and 50% respectively compared with the control. Glucose-6-phosphate dehydrogenase activity in erythrocytes of adult and old animals with diabetes decreased by 27% and 45% respectively compared with the control on 47-th day. The changes may be considered as the result of age-related disorders of glucose metabolism due to disturbances in free radical mechanisms. Glutathione reductase activity in erythrocytes of adult and old animals with diabetes decreased by 29% and 35% respectively compared with the control on 47-th day. Conclusion. We have determined when getting aged, the alloxan-diabetic rats demonstrate changes in the sensitivity of pyruvate kinase, glucose-6-phosphate dehydrogenase and glutathione reductase activities in erythrocytes resulted from the effect of diabetes mellitus factors (hyperglycaemia). We can suggest that glycaemic control is key purpose for older patients with type 1 diabetes in order to prevent of complication, which can be aggravated with age.
Analysis of the course of pregnancy, childbirth and the status of newborns born by displaced women
(Полтавський державний медичний університет, 2023) Kamilova, Nigar M.; Gulieva, Lamia A.; Камілова, Н. М.; Гулієва, Л. А.
Aim of the study: to analyse the effect of involuntary migration on the course of pregnancy, labour and delivery, and neonatal outcomes in pregnant women in the third generation. Materials and methods. This paper presents an analysis of a prospective study of the course of pregnancy, childbirth, and the condition of new-born infants born by displaced Azerbaijani women. A prospective analysis was made in the course of puberty and the state of reproductive health in 54 refugee women of early reproductive age, who were divided into two groups, aged 18-25 and 26-35 years. Results. A study conducted among third generation women revealed pronounced negative trends in both somatic and reproductive health among refugee women in this group. Their average age ranged from 18 to 35. Most of the patients, 37 (68.5%), were born with a birth weight of less than 3000g. The mean age of parents at the time of girl's birth was 25.4±2.26 years for mothers, 26.9±5.29 years for fathers, i.e., in the immediate post-stress period. The subjects are characterized by menstrual cycle dysfunction (63%-29.5%). Hypermenorrhoea occurs in 46% of the subjects, primary amenorrhoea in 8.2%, and secondary amenorrhoea in 3.3%. There is also a high percentage of inflammatory diseases of the female genital organs (93.4%). A high percentage of somatic pathologies is found in the female patients: iron deficiency anaemia - 77.8%, gastrointestinal diseases - 48.1%, thyroid diseases - 9.3%, urinary diseases - 22.5%, helminthiasis - 16.7%, etc. Sexually transmitted infections were diagnosed in chronic form in 14 patients. The rate of placental insufficiency of grade 1a and 1b was diagnosed in 29%-16%, and delayed foetal development of grade 1 and 2 in 12%-8% respectively. Asphyxia (27%), newborn hypotrophy (7.5%), prematurity (18%) is characteristic of the newborns of this group of patients. Conclusion. Analysis of the results of a comprehensive study of the health of pregnant refugee women has shown that even in the third generation the stress associated with forced migration of the older generation and adverse socio-medical problems are reflected in the formation and functioning of the reproductive age of the younger generation.
Anatomical basis of biomechanical properties of super ficialtissues of the anterior abdominal wall
(Українська медична стоматологічна академія, 2020-12) Drabovskiy, V. S.; Kerbazh, N. R.; Akeyshi, A. K.; Rybalka, Ya. V.; Драбовський, Віталій Сергійович; Кербаж, Нізар Ріда; Акєйші, Айюб Камал; Рибалка, Ярослав Володимирович; Драбовский, В. С.; Кербаж, Низар Рида; Акейши, Айюб Камал; Рыбалка, Ярослав Владимирович; Драбовський, Виталий Сергеевич
Biomechanics is a science that studies the mechanical properties of tissues, individual organs and systems and the body as a whole. The unique mechanical properties of the skin provide the function of support and protection of internal organs through the skin mobility and elasticity. This feature of the skin is determined by its microstructural organization and arrangement of connective tissue fibres. The mechanical properties of the skin are mainly determined by the collagen-rich dermis. The mechanics of the dermis, in turn, depends on the structure, density and direction of collagen fibres. Each biological tissue is able to acquire deformation properties i.e. stretching or contraction. At each stage of deformation in the tissues of different topographic and anatomical areas there are changes in histoarchitectonics (within the plastic characteristics, and outside these parameters). Different structural interactions are expressed by different mechanical factors, which are adequate to the magnitude and direction of tensile forces (deformation vectors), form the typical features of the connective tissue matrix of abdominal wall tissues. Normalization of the direction of tissue stress vectors, uniform distribution of the direction and force of deformation prevent microstructural rearrangement of the surface tissues of the abdominal wall. Dynamic changes in the histological structure and biomechanical behaviour of the skin are closely related to the aging process, hormonal background, mechanical factors: physiological stretching of the skin during rapid growth in adolescence, pregnancy, overweight (or rapid weight loss), under the influence of physical load and wound healing. All these factors lead to connective tissue remodelling. Thus, the skin has a complex three-dimensional morphological structure; it is subjected to prolonged exposure to internal and external factors that determines its mechanical properties.
Assessment of essential communicative skills of first year medical students
(Полтавський державний медичний університет, 2021) Ivanchenko, O. Z.; Melnikova, O. Z.; Lurie, K. I.
The purpose of the presented work is to assess the development and progression of basic communication skills of the 1st year students of the medical faculty that can serve as the basis for their further improvement over the course of professional education. The general level of students' communication skills was assessed using the test "Diagnosis of communicative and organizational tendencies"; the general level of empathy and the degree of its manifestation in communicating with different categories of people were studied using the Yusupov I. test; types of behavior in conflicts were estimated using the Thomas test. The research findings were processed according to the keys suggested by the authors of the tests. The study has shown that the majority of students (66%) demonstrates a high and very high level of general communication, while 18% of participants have low and very low levels of communication skills. An adequate general level of empathy was found in 91.7% of 1st year students, moreover, it was high in the remaining 8.3% of students. All respondents have shown the greatest empathy towards parents (average level 10.1 out of 15 possible scores), strangers (9.2) and children (8.9), while the level of empathy for older people was 6.9 points. Determining the strategy for resolving conflict situations has revealed that 1st year students most often choose a compromise (22.5% boys and 24% girls), the strategy of avoidance ranks the second position and is commn among the girls (23%, while the boys exhibit rivalry (21.8%), cooperation is chosen mainly by the girls (19.8%). The results of the study have shown that high levels of general communication and empathy could contribute to the choice of a future profession by the students and need only the right support during their graduate training program. Much attention should be paid to fostering empathy for the elderly people and the choice of cooperation as the best conflict resolution strategy between the parties during educational process at the university.
Assessment of the stress and metabolism levels by using electro-photonic emission analysis method in ukrainian military personnel after frontline service
(Полтавський державний медичний університет, 2023-05) Nevoit, G. V.; Korpan, A. S.; Nastroga, T. V.; Kitura, O. Ye.; Sokolyuk, N. L.; Lyulka, N. A.; Potiazhenko, М. М.; Невойт, Ганна Володимирівна; Корпан, Ангеліна Сергіївна; Настрога, Тетяна Вікторівна; Кітура, Оксана Євгенівна; Соколюк, Ніна Людвігівна; Люлька, Надія Олександрівна; Потяженко, Максим Макарович
This study aims to explore the potential of the Electrophotonic Emission Analysis (EPEA) method as an instrumental approach for studying stress in biomedical research. Specifically, the study focuses on assessing the stress levels of Ukrainian military personnel returning from the front using the EPEA method. Conciusions: The EPEA method proved effective in instrumentally detecting increased levels of stress and anxiety in 96% of military personnel, 93% of functionally healthy individuals, and 87% of patients with non communicable diseases in the study. It is of fundamental importance for scientific and systemic medicine to establish that changes in biophoton emission can be observed in patients with non-communicable diseases, including military personnel. Significant differences in total energy levels were found between patients and functionally healthy individuals, with EPEA results showing 41±4.25 J and 43.86±4.87 J respectively for pa tients and functionally healthy people (p<0.0001). Electro-photonic emission analysis is a promising, valid, accessible instrumental method for biomedical research.
Chronic pyelonephritis as a precipitating factor of hepatorenal syndrome in patients with alcoholic liver cirrhosis
(Полтавський державний медичний університет, 2021) Rusnak, I. T.; Slyvka, N. O.; Akentiev, S. O.; Berezova, M. S.; Kulachek, V. T.; Al Salama, M. V. O.; Rovinskyi, O. O.
Introduction. Most attempts to assess renal failure in alcoholic liver cirrhosis have so far focused on acute kidney injury and on the hepatorenal syndrome in particular. However, there are still limited data on the prevalence and clinical impact of chronic kidney disease in cirrhosis. Objectives. This study aimed to assess the influence of chronic pyelonephritis on the incidence of hepatorenal syndrome in patients with alcoholic liver cirrhosis. Material and methods. 165 patients with decompensated alcoholic liver cirrhosis and concomitant chronic pyelonephritis were enrolled in the study. They were divided into two groups according to the presence or absence of chronic pyelonephritis: group 1 had alcoholic liver cirrhosis only (n=82), group 2 had alcoholic liver cirrhosis + chronic pyelonephritis (n=83). Results. The general bacterial infections were more common in group 1 patients. The spectrum of the most frequent bacterial complications in the examined patients typical for alcoholic liver cirrhosis was as follows: the share of urinary tract infection made up 16.0% (95% confidence interval 14.4-27.9), pneumonia constituted 16.7% (95% confidence interval 10.5-22.7, bacteremia made up 4.0% (95% confidence interval 7.7-38.6), the share of skin infections (erysipelas) was 2.7% (95% confidence interval 0.7-6.6). Other infections including pulmonary tuberculosis, lung abscess, right leg abscess, osteomyelitis, bedsores, were less common (6.7%). Spontaneous bacterial peritonitis, taking into account all options, was found in 6 cases (10.5%, 95% confidence interval 4.0-21.5). As expected, the incidence of hepatorenal syndrome within 14 days of inpatient onset was almost twice higher in group 2 – 22 cases (27%), than in group2 – 13 cases (16%). The group 2 demonstrated a more severe course of alcoholic liver cirrhosis on the Child-Pugh scale compared with group I (class B - 29.9%; class C - 70.1% against class B - 46.4%; class C - 53, 6% ); the differences were statistically significant (χ2 = 4.30, p = 0.038). In patients of group 2, the lethal outcome in the hospital occurred in 6 (8.9%) cases. Conclusions: The results of the present study confirm the role of chronic pyelonephritis as one of the major precipitating factors of hepatorenal syndrome incidence in patients with alcoholic liver cirrhosis. This fact should be considered when making the treatment plan for these patients.
Circadian dynamics of optic density of melatonin receptors in the neurons of hypothalamic supraoptic nucleus in rats under altered photoperiod
(Українська медична стоматологічна академія, 2019) Vlasova, K. V.; Bulyk, R. Ye.; Власова, К. В.; Булик, Р. Є.; Власова, Е. В.; Булык, Р. Е.
Introduction. Melatonin production is considerably suppressed by light and affects the ability to transfer daily rhythm information from the hypothalamus to other neural target sites and thus alters the expression of some biological rhythms. The hormone controls the state of the hypothalamic-pituitary system and endocrine gland activity through melatonin receptors (membrane, cytosolic and nuclear ones). In addition, using a mechanism of the feedback, it interferes with the activity of supraoptic nucleus of the hypothalamus, which regulates water-salt metabolism and responses to stress. Objective: to provide quantitative circadian characteristics of melatonin receptors density in the neurons of the hypothalamic supraoptic nucleus of rats being under light stimulation as well as the correction of changes after injecting exogenous melatonin. Material and methods. The experiments were conducted on 60 white mongrel mature male rats weighing 150 – 180 g. The test animals were divided into 3 parts each with 2 groups, kept under the conditions of standard light regime, hyperilluminated and the injection of exogenous melatonin and day-round lighting within 7 days. To perform immunohistochemical methods, we used polyclonal antibodies to melatonin 1A receptors produced by Abcam and streptavidin biotin visualization system LSAB2 (peroxidase mark + diaminobenzidine) produced by Chemicon International Inc. We adhered to protocol standardization of methods for all sections. Additional staining of nuclei was performed with Mayer hematoxylin. Results. The indices of optical density of specific melatonin 1A receptors of supraoptic neurocytes staining obtained in the intact group (at 02.00 AM- 0,488 ± 0,0024, at 02.00 P.M. - 0,464 ± 0,0023, p = 0.002) and in animals subjected to light stress (at 02.00 AM0,295 ± 0, 0019, at 02.00 P.M.- 0,286 ± 0,0018, p = 0,012) had a probable value and were characterized by a clear diurnal periodicity. In the group of animals with pineal gland hypofunction modulation (at 02.00 A.M.- 0,216 ± 0,0017, at 02.00 P.M. - 0,214 ± 0,0021, p> 0,05). Conclusions The density of 1A melatonin receptors in rat’s hypothalamic supraoptic neurons are normally characterized by an accurate circadian rhythm. The highest density of receptors is observed at 02.00 AM, and at 02.00 PM it is significantly lower (p = 0.002). Immunohistochemical studies revealed that under inhibition of pineal gland activity the circadian rhythm of melatonin receptors density in neurons of supraoptic nuclei of the hypothalamus gets disturbed, which is characterized by an incredible difference of indices in the tested periods of the day.
Comparative analysis of cd 44 expression in polyps and adenocarcinoma of distal colon
(Полтавський державний медичний університет, 2020) Shyshkin, M. A.; Шишкін, М. А.; Шишкин, М. А.
Introduction. CD44 is one of the most used markers of cancer stem cells in colorectal cancer. Even though, the questions of its diagnostic and prognostic value remain open. The aim of the study was to compare CD44 immunohistochemical expression levels in polyps and adenocarcinoma of the distal colon. Materials and methods. Histopathological and immunohistochemical studies of biopsies from 40 patients and surgical material of colorectal adenocarcinoma from 30 patients were carried out. Results. It was figured out that distal colonic polyps are characterized by membranous CD44 expression with the medians of relative areas of CD44+ cells equal to 60,24 (50,22; 70,22) % stromal cells and 15,67 (12,47; 19,47) % epitheliocytes. Colorectal adenocarcinoma is characterized by membranous-cytoplasmic CD44 expression with the medians of relative areas of CD44+ cells equal to 61,26 (42,58 ; 79,15) % stromal cells and 30,60 (24,56 ; 36,45 ) % cancer cells. Comparative analysis of the data obtained for the pTNM stages of colorectal adenocarcinoma revealed some significant differences. The median of CD44+ stromal cells area on the I stage equals to 31,41 (19,87 ; 42,15) % vs. the median of CD44+ stromal cells area on the II stage equals to 48,26 (35,44 ; 61,45) % , р ˂ 0,05; the median of CD44+ stromal cells area on the II stage vs. the median of CD44+ stromal cells area on the III stage equals to 78,36 (61,13 ; 80,06) % , р ˂ 0,05. The median of CD44+ cancer cells area on the III stage equals to 30,35 (21,19 ; 35,47) % vs. the median of CD44+ cancer cells area on the IV stage equals to 31,25 (30,22 ; 41,19) % , р ˂ 0,05. Moreover, it was revealed that the median of CD44+ epitheliocytes area in polyps two-fold smaller than the median of CD44+ cancer cells area in colorectal adenocarcinoma: 15,67 (12,47 ; 19,47) % vs. 30,60 (24,56 ; 36,45 ) % , р ˂ 0,05. Conclusions. Distal colonic polyps are characterized by membranous CD44 expression with the median of CD44+ epitheliocytes area that is two-fold smaller than the median of CD44+ cancer cells area. Colorectal adenocarcinoma is characterized by membranous-cytoplasmic CD44 expression with the median of CD44+ stromal cells area that significantly increases during the tumor progression from I to III stages and with the median of CD44+ cancer cells area that significantly go up during the tumor progression from III to IV stages.
Comparative analysis of the prevalence of normotension and hypertension in diagnosis of arterial hypertension accordingto esc/esh (2018) and acc/aha (2017) diagnosis in people with normal glucose metabolism, prediabetes and type 2 diabetes
(Полтавський державний медичний університет, 2023) Isgandar, M. A.; Gurbanov, Y. Z.; Huseynova, N. N.; Sultanova, S. S.; Mirzazade, V. A.
The comorbidity between hypertension with diabetes leads to an increase in the risk of death and cardiovascular events by 44% and 41%, respectively, compared with 7% and 9% risks in people suffering only from diabetes and having no hypertension. Purpose. To determine the effect of the criteria for the diagnosis of hypertension proposed by ACC/AHA (2017) and ESC/ESH (2018) on the detection of this disease in people with normal carbohydrate metabolism, prediabetes and type 2 diabetes mellitus. Materials and methods. A retrospective analysis of the database of the Azerbaijan Association of Endocrinology, Diabetology and Therapeutic Training was carried out. The data of 596 examined patients were analyzed, then three main groups were formed: a group with normal carbohydrate metabolism (n=99), a group with prediabetes (n=47), a group with type 2 diabetes mellitus (n = 450). The patients included in the study were divided into 2 samples: those, who have DM2; those, who have no DM2. Including the patients in the group of with DM2 was based medical history data about the presence of DM2 and / or taking hypoglycemic drugs. Results. When applying the ACC/AHA criteria (2017), normotension (systolic blood pressure <130 mmHg and diastolic blood pressure <80 mmHg) occurred in 40.4% (95% CI 30.69%; 50.12) of the control group, while 59.6% (95% CI 49.88; 69.31) there was arterial hypertension. 14.9% of the prediabetes group (95% CI 4.60%; 25.18)% had normotension according to the same criteria, while the majority of the participants in this group – 85.1 (95% CI 74.82; 95.40)% had arterial hypertension. In the type 2 diabetes mellitus group, normotension was found in 15.8 (95% CI 12.41%; 19.15)%, and arterial hypertension – in 84.2 (95% CI 80.85; 87.59)%. Thus, arterial hypertension was statistically significantly less common in the control group than in prediabetes (p <0.01) and type 2 diabetes mellitus (p <0.001). Conclusion. Thus, in people with normal carbohydrate metabolism, prediabetes and type 2 diabetes mellitus, the use of ACC/AHA diagnostic criteria (2017) led to a statistically significantly higher incidence of hypertension than when using the criteria for the diagnosis of hypertension according to ESC/ESH (2018).
Cтан та корекція цитоенергетичного забезпечення в комплексі інтенсивної терапії синдрому гастроінтестинальної недостатності у новонароджених
(Українська медична стоматологічна академія, 2018) Холод, Дмитро Анатолійович; Шкурупій, Дмитро Анатолійович; Холод, Дмитрий Анатольевич; Шкурупий, Дмитрий Анатольевич; Shkurupiy, D. A.; Holod, D. A.
З метою оптимізації інтенсивної терапії у новонароджених із синдромом гастроінтестинальної недостатності шляхом обґрунтування застосування препарату бурштинової кислоти проведене рандомізоване клінічне дослідження за участю 110 новонароджених із синдромом гастроінтестинальної недостатності. До основної групи увійшли новонароджені, які в комплексі терапії отримували інфузію препарату із вмістом сукцинату натрію (n=66), до групи порівняння – новонароджені, яким застосування цього препарату не проводилось (n=44). У обстежених новонароджених на початку дослідження спостерігалась тенденція до формування лактат-ацидозу, при цьому не було встановлено значимого кореляційного зв’язку між виразністю синдрому гастроінтестинальної недостатності і показниками цитоенергетичного забезпечення. Наприкінці дослідження показники цитоенергетичного забезпечення в обох групах різнились за рахунок зменшення рівня молочної кислоти, її співвідношення відносно піровиноградної кислоти і збільшення активності сукцинатдегідрогенази у новонароджених, які додатково отримували препарат бурштинової кислоти. При цьому був встановлений прямий значимий кореляційний зв’язок між виразністю синдрому гастроінтестинальної недостатності і рівнем молочної кислоти. Таким чином, у обстежених новонароджених із синдромом гастроінтестинальної недостатності формуються цитоенергетичні порушення, що відображається на прогресуванні і виразності даного синдрому. Призначення препаратів бурштинової кислоти дозволяє покращити стан клітинного енергозабезпечення, зменшуючи прояви лактатацидозу і призводять до зменшення виразності і прогресування синдрому гастроінтестинальної недостатності; the grounds to use amber acid, we carried out a randomized clinical trial involving 110 newborns with gastrointestinal insufficiency syndrome. The main group included newborns who received infusion with sodium succinate (n = 66) as a part of integrated therapy; the control group included the newborns who were not administered the above mentioned medication (n = 44). At the beginning of the study we observed a tendency towards the formation of lactate acidosis in the examined newborns, while no significant correlation was established between the severity of the gastrointestinal insufficiency syndrome and the indicators of cell energy supply. At the end of the study, the cell energy supply indicators varied in both groups by reducing the level of lactic acid, its ratio regarding pyruvic acid and increase in the activity of succinate dehydrogenase in newborns, who received amber acid in addition to the standard treatment. At the same time we observed a direct significant correlation between the severity of the syndrome of gastrointestinal insufficiency and the level of lactic acid. Thus, the examined newborns with gastrointestinal insufficiency syndrome are found to start developing cell energy disorders that is reflected in the progression and severity of the syndrome. Administration of amber acid medications helps to improve the state of cell energy supply, reducing the manifestations of lactate acidosis and declining the severity and progression of gastrointestinal insufficiency syndrome.
Distance learning technologies of postgraduate dental education system
(Полтавський державний медичний університет, 2021) Maksymenko, A. I.; Максименко, Аліна Іванівна
The implementation of distance learning is carried out with the help of modern systems of distance education. They allow to teach and to assess the knowledge of interns and doctors quickly and easily, regardless of their location. The aim of the study. A comparative review of the most well-known distance learning platforms, wich are designed to organize the learning process and control learning with the help of Internet technology. System of distance education is a virtual classroom with the possibility to train interns and doctors from different regions of Ukraine at the same time. There are many educational platforms for distance learning nowadays, such as Moodle (Australia), iSpring Learn LMS (Russia), Collaborator (Ukraine), eTutorium LMS (Ukraine), Opigno (Belgium), Atutor (Canada). Moodle is a free platform that allows users to create individual courses. It supports more than 100 languages. iSpring Learn LMS is a simple and user-friendly system that is a paid alternative to Moodle. Collaborator is a platform that works effectively on all modern devices and browsers and is virtually independent of the software of the user's device. eTutorium LMS is a virtual distance learning system that allows to create an online course of any complexity quickly. Opigno is a modern free distance education system based on Drupal (a popular content management system). Atutor, like Moodle, is an open web-based e-learning system. Conclusion. Distance learning systems differ not only functionally, but also in the way they solve problems. The simplicity of use of the platform depends on the degree of its adaptation to the needs of the user and the ability to use all existing features and functions of the system.
Dynamic changes of in incidence of ixodes tick bite cases in adult population of Vinnitsa region, Ukraine
(Полтавський державний медичний університет, 2021) Panko, V.; Boiarska, Z.; Berezovskiy, I.; Mushit, S.
Not all ticks carry dangerous pathogens, but their bites can threaten humans with diseases such as Ixodes tick-borne borreliosis (Lyme disease), Miyamoto disease, tick-borne viral encephalitis and about 150 other nosological entities. The season of life activity of Ixodes ticks begins in March and usually lasts until November and has two pronounced seasonal peaks during the year: over April – May and August – September. The relevance of the investigation is determined by the lack of the information on the incidence of tick bite cases among the population in Ukraine, and there no well elaborated sets of measures in order to prevent this problem. The purpose of this study is to provide a detailed analysis of the incidence of Ixodes tick bite cases among the adult population in the Vinnytsia region and in the city of Vinnytsia, to compare the number of detected cases for the period from 2013 to 2018, and to identify the most reliable and available prevention methods. The research was conducted at the laboratory of the municipal non-profit enterprise "Vinnytsia City Hospital G1" (KNP "VMKL"); statistical data were provided by the State Regional Sanitary and Epidemiological Station. We compared the prevalence of the disease in different districts of the Vinnytsia region and its insidence for the period from 2013 to 2018. During this period, the number of detected cases of tick bites resulted in the occurrence of various dangerous diseases went up from 53 to 317 cases among the entire adult population of the Vinnytsia region. For all years except 2018, the highest incidence rate was consistently observed in the Mogilev-Podilsk district district, even exceeding the level in more densely populated and large areas. Over the period from 2013 to 2018, the number of detected cases of tick bites in the adult population that resulted in various dangerous diseases rapidly and significantly increased. This necessitates to elaborate the set of preventive measures and to educate the population of all ages. Much attention should be paid to promoting vaccination as the only reliable way and the development of sanitary and hygienic recommendations for residents of municipal and rural areas (in the spring and summer).
Effects of silver nanoparticles on penty lenetetrazol-induced kindled seizures in rats
(Українська медична стоматологічна академія, 2019) Pervak, M. P.; Первак, М. П.
Silver nanoparticles are used for delivering neurotropic agents to brain, increasing the bioavailability of insoluble drugs as well as for brain tumours treatment. Their effects upon brain excitability, especially under conditions of chronic epilepsy modelling are underinvestigated. The purpose of the study was to study the effects of silver nanoparticles upon pentylenetetrazol-induced kindled seizures in rats. Material and Methods. Experiments were performed on Wistar rats kindled during four weeks with pentylenetetrazol administrations in a dose of 30.0 mg/kg, i.p. Only rats, which demonstrated generalized clonic-tonic seizures in a response to each of three last epileptogen injections, were included into the study. Nanoparticles and ionized argentum were administered in a dosage of 0.2 mg/kg, i.p. Observation was performed in early and postponed period of kindling – in 24 h and three weeks from the moment of kindling induction, correspondently. Results and discussion. The calculation of the pentylenetetrazol dose, which effectively induced seizures in 50 % of kindled rats (ED50), was performed in kindled animals in 24 h from the moment of last epileptogen administration was 22.0 mg/kg. In postponed period of kindling (three weeks from the moment of last pentylenetetrazol administration in a dose of 30.0 mg/kg, i.p.), ED50 of pentylenetetrazol was 16.0 mg/kg. ED50 of pentylenetetrazol, which induced clonic seizures in 50 % animals at the early stage of kindling, was recalculated following the administration of ionized and nanoparticle forms of silver, and were less when compared with such ones determined in the control group by 13.5 % (P>0,05) and 26.0 % (P<0,05), respectively. In the postponed period of kindling ED50 of ionized and nanoparticle forms of silver were less when compared with control by 20.0 % (P<0.05) and by 42.0 %, respectively (P<0.05). Significant difference was noted between groups treated with ionized and nanoparticle silver (P<0.05). The net intensification of pentylenetetrazol seizure manifestations was seen in kindled rats treated with silver nanoparticles, when seizure discharges amplitude achieved 1.0-1.2 mV and frequency of 3/sec, which exceeded the ones in rats treated with ionized silver. Conclusion. Silver nanoparticles induce the increase in seizures severity in pentylene
Fostering clinical thinking in dental doctors through post-graduation professional training
(Українська медична стоматологічна академія, 2019-05) Падалка, Аліна Іванівна; Падалка, Алина Ивановна; Padalka, A. I.; Костенко, Вікторія Геннадіївна; Костенко, Виктория Геннадиевна; Kostenko, V. G.; Шешукова, Ольга Вікторівна; Шешукова, Ольга Викторовна; Sheshukova, O. V.
During the internship training, future dental doctors expand knowledge about understanding the pathogenetic mechanisms of various pathological conditions or illnesses. Most contemporary effective diagnosis and treatment approaches are based on clinical thinking skills, the link between sciences and practice. The purpose of this study is to investigate and analyze the peculiarities of building up clinical thinking skills in future paediatric dentists at the postgraduate stage of their training. Developing clinical thinking skills in dental students typically starts since their junior years at the medical university, when they begin studying basic sciences. The background obtained during undergraduate training should be enriched in order to improve recognizing a wide variety of diseases and conditions through the internship training course. Professional thinking skills are fostered at the chair-side practice, when dealing with patients under the guidance of experienced mentors and clinicians. Clinical thinking switches on since the first minutes of doctor-patient interaction and goes on through the treatment course. At the postgraduate stage, it is essential to continue developing the holistic vision of the patient that will help to combine the evidence-based standards of medical care and patient-centred approach (personalized medical care). Conclusion. Thus, the training activities for dental intern doctors should be designed in order to maximally contribute to fostering clinical thinking skills. The mentors and teaching assistants must have clear and deep understanding of what factors can promote this process, and what can not. Among the most significant methodological achievements of postgraduate education is the emphasis on the development of critical and creative thinking skills, which are most clearly manifested by the creativity of clinical thinking. Під час навчання в інтернатурі лікарі-інтерни розширюють той обсяг знань, що необхідний для розуміння патогенетичних механізмів виникнення будь-якого патологічного стану або хвороби. Для успішного розпізнавання хвороби і ефективного лікування необхідно володіти клінічним мисленням, як сполучною ланкою між теорією і практикою. Мета роботи. Розглянути та проаналізувати особливості формування клінічного мислення у лікарів-стоматологів дитячих на післядипломному етапі навчання. Формування клінічного мислення у лікарів-стоматологів починається вже на перших курсах навчання при опануванні базових дисциплін. Накопичені знання дозволяють використовувати цей багаж знань при розпізнаванні різних захворювань у процесі навчання в інтернатурі. Професійне мислення виховується у процесі спілкування інтерна, викладача і пацієнта біля крісла хворого. Клінічне мислення починається з перших хвилин спілкування з хворим і триває протягом усього лікувального процесу. На післядипломному етапі навчання у лікаря-інтерна необхідно формувати цілісне уявлення про хворого, що дозволить органічно поєднувати стандарти медичної допомоги з індивідуальним підходом. Висновок. Навчальний процес повинен будуватися таким чином, щоб максимально сприяти формуванню клінічного мислення у лікарів-інтернів. Викладач повинен знати і розуміти, які фактори сприяють цьому процесу, а які ні. Значущим методологічним досягненням післядипломної освіти є упор на розвиток творчих здібностей лікарів-інтернів, які найбільш яскраво проявляються креативністю клінічного мислення. Во время учебы в интернатуре врачи-интерны расширяют тот объем знаний, который необходим для понимания патогенетических механизмов возникновения любого патологического состояния или болезни. Для успешного распознавания болезни и эффективного лечения необходимо обладать клиническим мышлением, как связующим звеном между теорией и практикой. Цель работы. Рассмотреть и проанализировать особенности формирования клинического мышления у детских врачей-стоматологов на последипломном этапе обучения. Формирование клинического мышления у врачей-стоматологов начинается уже на первых курсах обучения при овладении базовыми дисциплинами. Накопленные знания позволяют использовать этот багаж знаний при распознавании различных заболеваний в процессе обучения в интернатуре. Профессиональное мышление воспитывается в процессе общения интерна, преподавателя и пациента у кресла больного. Клиническое мышление начинается с первых минут общения с больным и продолжается в течение всего лечебного процесса. На последипломном этапе обучения у врача-интерна необходимо сформировать целостное представление о больном, что позволит органично сочетать стандарты медицинской помощи с индивидуальным подходом. Вывод. Учебный процесс должен строиться таким образом, чтобы максимально способствовать формированию клинического мышления у врачей-интернов. Преподаватель должен знать и понимать, какие факторы способствуют этому процессу, а какие нет. Значимым методологическим достижением последипломного образования является упор на развитие творческих способностей врачей-интернов, которые наиболее ярко проявляются креативностью клинического мышления.
General biological patterns of the structure of human major and minor lacrimal glands and under-researched aspects of their morphology
(Українська медична стоматологічна академія, 2019) Katsenko, A. L.; Sherstiuk, O. A.; Svintsytska, N. L.; Piliuhin, A. V.; Piliuhin, V. A.; Каценко, Андрій Любославович; Шерстюк, Олег Олексійович; Свінцицька, Наталія Леонідівна; Пілюгін, Андрій Валентинович; Пілюгін, Валентин Олексійович; Каценко, Андрей Любославович; Шерстюк, Олег Алексеевич; Свинцицкая, Наталья Леонидовна; Пилюгин, Андрей Валентинович; Пилюгин, Валентин Алексеевич
The article discusses the fundamental and modern data on the structure and functions of human large and small lacrimal glands. The palpebral lobe of the human lacrimal gland consists of numerous fairly large lobules, which are almost of identical structure. Each lobule has several axial excretory intralobular ducts. Their branches along the whole length are surrounded by secretory epithelial components in the form of terminal sections and the corresponding finniest terminal lacrimal ducts that are grouped into the grapebunch structural elements. Blood supply to the capillary network of the lacrimal gland and its alveoli is carried out by several arterioles that run through very large interstitial spaces rich in fatty tissue. Each arteriole supplies several adjacent lobules. The network of capillaries can be described as integral and is not divided into segments that would correspond to sublobular units. Blood capillaries are located in the spaces between several adjacent terminal portions. Thus, the fact of close syntopic similarity of the intralobular excretory ducts and postcapillary venules has been confirmed. The article poses the appropriateness of more detailed morphological studying of anatomical and topographic characteristics of the lacrimal glands. If has been found out the lack of information on the cellular composition of the wall of the excretory ducts of the lacrimal gland, both in humans and in laboratory rats, in order to compare their morphology and the quality of the secretion they produce. It would be interesting to compare the morphology of the human lacrimal glands and the garder gland in rats to identify general biological characteristics of the structure and functioning; В статье рассмотрены фундаментальные и современные данные о структуре и функции больших и малых слезных желез человека. Пальпебральная доля слезной железы человека состоит из многочисленных достаточно крупных долек, которые имеют практически идентичный принцип устройства. Каждая долька имеет несколько осевых выводных внутридольковых протоков. Их разветвления на всем протяжении окружены секреторными эпителиальными компонентами в виде концевых отделов и соответствующих им мельчайших терминальных слезных протоков, образующих в совокупности структурные элементы гроздевидной формы. Доставка крови к капиллярной сети долек слезной железы и ее mальвеол осуществляется несколькими артериолами, которые проходят в очень крупных интерстициальных промежутках богатых жировой тканью. Каждая такая артериола кровоснабжает несколько смежных долек. От нее берут свое начало прекапиллярные артериолы, непосредственно питающие кровью альвеолы дольки. Сеть капилляров едина и не разделяется на блоки, которые соответствовали бы субдольковым единицам. Кровеносные капилляры располагаются в пространствах между несколькими смежными концевыми отделами. Таким образом, подтверждается факт тесного синтопического соответствия внутридольковых выводных протоков и посткапиллярных венул. Доказана необходимость в проведении более детальных морфологических исследований, анатомо-топографических особенностей слезных желез. Требуется дальнейшее изучение клеточного состава стенки выводных протоков слезной железы, как у человека, так и у лабораторной крысы с целью сравнения их морфологии и качества вырабатываемого ими секрета. Интересным было бы сравнить морфологию слезных желез человека и гардеровой железы крысы с целью выявления общебиологических черт строения и функции; У статті розглянуті фундаментальні та сучасні дані про структуру та функції великих і малих сльозових залоз людини. Пальпебральна частка сльозової залози людини складається з численних досить великих часточок, які мають практично ідентичний принцип улаштування. Кожна часточка має кілька осьових вивідних внутрішньодолькових проток. Їх розгалуження на всьому протязі оточені секреторними епітеліальними компонентами у вигляді кінцевих відділів і відповідних їм найдрібніших термінальних сльозових протоків, що утворюють в сукупності структурні елементи гроноподібної форми. До- ставка крові до капілярної мережі часточок сльозової залози і її альвеол здійснюється декількома артеріолами, які проходять в дуже великих інтерстиціальних проміжках, багатих на жирову тканину. Кожна така артеріола кровопостачає кілька суміжних часточок. Від неї беруть свій початок прекапілярні артеріоли, що безпосередньо живлять кров'ю альвеоли часточки. Мережа капілярів єдина і не поділяється на блоки, які відповідали б субдольковим одиницям. Кровоносні капіляри розташовуються в просторах між декількома суміжними кінцевими відділами. Таким чином, підтверджується факт тісної синтопічної відповідності внутрішньодолькових вивідних протоків і посткапілярних венул. Доведена необхідність в проведенні більш детальних морфологічних досліджень, анатомо-топографічних особливостей сльозових залоз. Потрібне подальше вивчення клітинного складу стінки вивідних протоків сльозової залози, як у людини, так і у лабораторних щурів з метою порівняння їх морфології і якості секрету, що ними виробляється. Цікавим було б порівняти морфологію сльозових залоз людини і гардерової залози щура з метою виявлення загальнобіологічних рис будови і функції.
Generalized histostructural and morphometric characteristics of the frontal sinus of human in health
(Полтавський державний медичний університет, 2023) Serbin, S. I.; Dubyna, S. O.; Bondarenko, S. V.; Sovgyrya, S. M.; Koptev, M. M.; Сербін, Сергій Ігорович; Дубина, Сергій Олександрович; Бондаренко, Станіслав Володимирович; Совгиря, Світлана Миколаївна; Коптев, Михайло Миколайович
The aim of our study is to determine the histo-topographic and morphometric features of the structural components of the mucous membrane of the human frontal sinus on different walls. Materials and methods: to achieve the goal, we used a set of methods for morphological studies including the method of serial semithin sections, histochemical studies, and the morphometric method. The thickest mucous membrane on the lower wall has an average epithelium thickness of 51.45±1.59 μm on the left and 50.78±1.64 μm on the right. On the left, the lamina propria thickness is 42.49±5.34 μm, and on the right, it is 43.03±2.22 μm. The submucosal base has a thickness of 423.67±21.33 μm on the left and 426.45±16.77 μm on the right, which is four times higher than the average thickness of the back wall and septum (p<0.05). The average diameter of the lumen of the resistive and capacitive links of the hemomicrocirculatory channel is the largest in the mucous membrane of the front wall of the human frontal sinus (respectively, 11.16±0.37 μm on the left and 11.34±0.31 μm on the right (p<0.05); 7, 63±0.08 μm on the left and 7.57±0.09 μm on the right), the smallest is in the mucous membrane of the back wall (respectively, 6.96±0.19 μm on the left and 6.56±0.35 μm on the right; 8.82±0.16 μm on the left and 9.02±0.43 μm on the right (p<0.05)). Exchangeable hemomicrovessels with the smallest diameter had a measurement of 3.89±0.08 μm on the left and 4.19±0.02 μm on the right (р<0.05) within the specified area. The study revealed the largest capillaries in the mucosal lining of the lower wall, which measured 4.88±0.07 μm on the left and 4.73±0.12 μm on the right (p<0.05). Furthermore, complex alveolar-tubular glands were evident in the submucosal base of the mucosal lining in all walls of the designated sinus. It has been found that the glands on the lower wall have the largest diameter of the terminal sections, whereas those on the septum have the smallest. On the left, the glands on the lower wall have a diameter of 30.42±2.36 μm, while those on the septum have a diameter of 25.42±1.68 μm. On the right, the corresponding measurements are 31.01±1.34 μm and 25.89±1.38 μm (p<0.05). In conclusion, this study highlights the distinct differences in gland diameter and type among different sections of the organ. The glands on the front and back comprised a mixture of protein and mucous types, while two types were identified on the lower wall and septum. The statistical processing of the obtained histo-topographic, histological, histochemical, and morphometric data significantly enhances the comprehension of the microscopic structure of the mucous membrane in the frontal sinus. These findings can be important in modern morphology and otorhinolaryngology.
In vitro антирадикальна активність безклітинних екстрактів bifidobacterium bifidum та lactobacillus reuteri
(Полтавський державний медичний університет, 2020) Книш, О. В.; Нікітченко, Ю. В.; Knysh, O. V.; Nikitchenko, Yu. V.
Пробіотичні мікроорганізми здатні виявляти антиоксидантну активність. Більшість корисних ефектів пробіотиків зумовлені дією їх дериватів (структурних компонентів та метаболітів). Метою роботи було дослідити антирадикальну активність безклітинних екстрактів, що містять деривати бактерій пробіотичних штамів Bifidobacterium bifidum 1 та Lactobacillus reuteri DSM 17938, за здатністю поглинати гідроксильні радикали в модельній системі їх генерації. Безклітинні екстракти отримували з дезінтегратів та культур пробіотичних бактерій, що культивувалися у дезінтегратах власних клітин. Препаратом порівняння було обрано метабіотик HYLAK FORTE. Безклітинні екстракти L. reuteri за вмісту в інкубаційному середовищі 20% об. забезпечували поглинання гідроксильних радикалів на рівні 75 – 90 %, а екстракти B. bifidum – на рівні 50 – 60 %. Метабіотик такий же рівень поглинання гідроксильних радикалів забезпечував за нижчого у 1,5 – 2,5 рази вмісту у середовищі інкубації. Більш виражену антирадикальну активність виявили екстракти з дезінтегратів, ніж екстракти з культур пробіотиків. Для екстрактів і метабіотика визначено концентрації 50 % поглинання гідроксильних радикалів (ІК50) з розрахунку на масу сухого залишку. За цим показником найбільш ефективним виявився екстракт з дезінтеграту L. reuteri (ІК50 = 1,57 мг/мл). Решта досліджених дериват-вмісних продуктів розташувалися в порядку зменшення антирадикальної активності наступним чином: екстракт з дезінтеграту B. bifidum (ІК50 = 1,64 мг/мл) >екстракт з культури B. bifidum (ІК50 = 1,75 мг/мл) > екстракт з дезінтеграту L. reuteri (ІК50 = 1,86 мг/мл) > HYLAK FORTE (ІК50 = 11,03 мг/мл). Отже, всім дослідним екстрактам притаманна антирадикальна активність по відношенню до найбільш реакційно здатного гідроксильного радикалу. Отримані результати спонукають до подальшого вивчення антиоксидантних властивостей безклітинних екстрактів L. reuteri та B. bifidum з метою з’ясування механізмів їх біологічної активності та обґрунтування доцільності їх терапевтичного застосування.

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