Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії, Том 18, вип. 3 (63)
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Перегляд Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії, Том 18, вип. 3 (63) за Ключові слова "577.175:577.151:616.379 – 008.64] – 092.9"
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Документ Melatonin improves mitochondrial function and decreases oxidative stress in gums of diabetic rats(Українська медична стоматологічна академія, 2018) Yaremii, I. M.; Kushnir, O. Yu.; Khlus, K. M.; Яремий, И. М.; Кушнир, А. Ю.; Хлус, К. М.; Яремій, І. М.; Кушнір, О. Ю.; Хлус, К. М.It is known that melatonin not only carries out control of circadian and seasonal biorhythms in the human body, but also helps to maintain oxidative antioxidant homeostasis and normoglucemia in the body. The purpose of the study was to determine the effect of melatonin on the content of reduced glutathione and the activities of glutathione peroxidase, catalase and superoxide dismutase, as well as the content of TBC reactive compounds and protein carbonyl content in the cytosol, and the activities of succinate dehydrogenase and H+-ATP-ase in the mitochondria of the gums of rats with alloxan diabetes mellitus. Research methods. Animals were divided into 5 groups: 1) control; 2) rats with apparent diabetes – basal glycemia levels ≥ 8.0 mmol / l; 3) rats with apparent diabetes who, from day 5 after administration of aloxane, received daily melatonin 10 mg / kg of weight during 7 days daily at 8:00 per os; 4) rat with impaired glucose tolerance - basal glycemia level ≤ 6.9 mmol / l; 3) rats with impaired glucose tolerance, which were similarly administered within 7 days of melatonin. Results and discussion. In gingival tissues of rats with apparent diabetes, reduction in the content of reduced glutathione by 30% and increased activity of glutathione peroxidase by 32% were observed, respectively, when compared with control rats. The activity of catalase and superoxide dismutase decreased by 18% and 46% in the group of diabetic rats than in the control group. In the group of animals with impaired glucose tolerance, the activity of catalase was 25% higher than control. The content of TBC reactive compounds increased in groups of diabetic rats and with impaired glucose tolerance by 65% and 36% respectively, while the level of oxidized proteins in animals with diabetes increased by 52% compared to control. In the mitochondrial fraction of gum cells, decrease in the activity of succinate dehydrogenase and H + -ATPase in animals with diabetes was found to be 68% and 41%, respectively, as compared to control. Weekly daily administration to rats with apparent diabetes melatonin at a rate of 10 mg / kg contributed to the normalization of the glutathione system and basal glycemia we studied. Conclusion: These results demonstrate that melatonin supplementation prevents gingival mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.