New molecular-biological and genetic methods applied in African countries

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Ескіз

Дата

2025

Назва журналу

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Назва тому

Видавець

Тернопільський національний медичний університет

Анотація

Innate pathology level in Africa exceeds high-income countries on 20%. Hemoglobinopathies, G6PD deficiency, albinism, neural tube defects are prevalent in sub-Saharan Africa. Nigerian population having sickle-cell disease is the biggest in the world, comprising 22-25% of all the inhabitants. 2:100 of Nigerian children or 2- 3% of all newborns have it. Muscular-skeletal and gastro-intestinal congenital anomalies are prevalent in the Nigerians among all congenital diseases. Inherited myopathies were found to be dominant in the North Africa. There exist dys- advantages of old investigative methods defining essentiality to create, introduce in healthcare system and to manage the new ones – informative, rather easily to be performed and possessing multiple advantages. The authors analyzed modern literature concerning new investigative methods for medical-biological and genetic researches in African countries. Materials and methods: scientific literary sources for 2019-2025 on the topic studied. Brief analysis of literary sources testifies to their rather big set and expressed opportunities. Following methods application was analyzed: Next-Generation Sequencing (NGS), Single Nucleotide Polymorphism (SNP) Genotyping, Sanger Sequencing, Fluorescence in situ Hybridization (FISH), Restriction Fragment Length Polymorphism (RFLP), Microarray Analysis, CRISPR- Cas9 gene editing, Reverse Transcription PCR (RT-PCR), Whole Exome Sequencing (WES).

Опис

Ключові слова

molecular biology, genetics, molecular-biological methods, genetic methods, next-generation sequencing (NGS), single nucleotide polymorphism (SNP) genotyping, sanger sequencing, fluorescence in situ hybridization (FISH), restriction fragment length polymorphism (RFLP), microarray analysis, CRISPR-Cas9 gene editing, reverse transcription PCR (RT- PCR), whole exome sequencing (WES), whole-genome sequencing, identifying mutations, chromosomal abnormalities detecting, genetic diversity, populational genetics, sickle cell anemia, specific genes’ sequencing, infectious diseases diagnostics, genetic variations’ analyzing at specific positions in the genome, disease susceptibility, malaria, pharmacogenomics, genetic disorders, cancer markers, HIV, leukemia, thalassemia, RNA, DNA, hepatitis, COVID-19, reverse transcription, revertase, reverse polymerase, rare inherited diseases, exons, exomes, complex diseases, diabetes, hypertension, hemoglobinopathies, G6PD deficiency, albinism, neural tube defects, muscular-skeletal anomalies, gastro-intestinal congenital anomalies, inherited myopathies, caspases, caspase 9

Бібліографічний опис

New molecular-biological and genetic methods applied in African countries / M. Fouzan, H. Mustafa, P. Entonu ; scientific supervisor : O. V. Tkachenko // XХІX Конгрес студентів та молодих учених «Майбутнє за наукою», 9–11 квітня 2025 р., м. Тернопіль, Україна. – Тернопіль, 2025. – С. 96.