Stress-Protected Effect of Torasemide in Acute Immobilization Stress in Rats

Abstract

The issue of search and selection the novel effective and, most importantly, safe methods of prevention and treatment of stress disorders remains one of the priorities for contemporary medical science. The paper was aimed at the morphological study of the rational use of torasemide as a stress protector. Based on bioethics regulations, 25 albino mature male rats aged were involved into morphological study. Group І involved intact animals; Group II involved animals that were exposed to acute immobilization stress without correction; Group III involved animals, exposed to stress after intraperitoneal administration of sodium chloride; Group IV involved animals, exposed to stress and corrected with mexidol; Group V involved rodents, exposed to stress and corrected with torasemide. After rats’ sacrifice, macroscopic and microscopic study of the lungs, liver, kidneys and spleen was performed. Histological preparations were stained with hematoxylin and eosin. Studies of the stress-protective properties of torasemide indicate its positive corrective effect on the structure of the lungs, liver and spleen in acute immobilization stress. However, morphologically, correction with torasemide in acute immobilization stress caused enhancement of stress-related renal alterations. Due to the adverse effects of torasemide on the histological structure of the kidneys under stress, its use as a stress protector is not considered rational.