Наукові праці. Кафедра патофізіології
Постійне посилання зібрання
Переглянути
Перегляд Наукові праці. Кафедра патофізіології за Назва
Зараз показуємо 1 - 20 з 247
Результатів на сторінці
Налаштування сортування
Документ Application of saliva crystalloscopy methods in the diagnosis of malignant tumours of salivary glands and sjogren disease(Published by IASHE London, 2015) Moskalenko, P.; Lakhtin, Yu.; Levkov, A. A.; Smeyanov, Yu.; Левков, Анатолій АнатолійовичIntroduction. Sjogren disease (SD) – organ-specifi c autoimmune disease of unknown aetiology, accompanied by the lesions of exocrine glands. Symptoms of xerostomia and xerophthalmia under SD may last for a long time remaining unrecognized, despite of their steady growth. Sjogren syndrome (SS) expresses in lymphoid infi ltration of salivary and lacrimal glands, which occurs in 5-25 %% of patients with systemic connective tissue diseases, more often with rheumatoid arthritis, less with systemic lupus erythematosus and other autoimmune diseases. At the heart of the pathogenic mechanisms of SD/SS is B-cell hyperactivity, that comes out in lymphoid infi ltration both secreting epithelial glands and other organs and tissues, in the formation of autoantibodies (RF, ANF, Ro / La) and cryoglobulins with monoclonal RF, and also in the development of predominantly B-cell MALT lymphomas in 5-16% of patients [3]. Diagnostics of salivary gland tumours is provided by a range of measures, the main role of which is in clinical analysis of available to the physician data [10]. One of the most notable trends in medicine in recent years is an active development and implementation in practice of noninvasive methods of diagnosis, defi ned primarily by the desire to obtain diagnostic information about the most important functions of the body by “bloodless” way and, if possible, without damage of natural barriers [8]. A number of methods of radiation diagnosis of salivary glands diseases were proposed, but they are not widely used in the clinic, because the frequency of diagnostic errors is 19 to 60% [9]. In recent years in clinical medicine the crystallographic research methods of various biological substrates become more widely used. The perspective of these methods use is determined by their high informational content, because the nature of crystallization refl ects quite reliably the peculiarities of pathological processes that occur in the body [2, 7]. This allows prompt and early diagnosis of diseases using completely safe method that does not require time-consuming and expensive equipment. In the available literature, we could not fi nd information on using crystallographic methods for diagnosis of salivary glands tumours, thus the purpose of the study was to improve the methods of early diagnosis of lymphoproliferative lesions of the salivary glands of patients with Sjogren disease and malignant tumours using crystallography and crystalloscopy of saliva. Materials and methods. We observed 12 patients with Sjogren disease, 83.3 % of which have uncomplicated tumours, and 16.7% - compound. We refer to uncomplicated the tumours that was found on the initial stages of SD/SS development, accompanied by lesions of the gastrointestinal tract (refl ux, indigestion, diarrhea, constipation), respiratory tract (chronic cough, recurrent bronchitis), kidney (proteinuria, tubular acidosis, interstitial nephritis, glomerulonephritis), other organs and systems (cutaneous vasculitis, peripheral neuropathy, lymphadenopathy, fever of non-infectious aetiology, dental caries, ulceration and clouding of the cornea, chronic infectious and ulcerative processes in the oral cavity and sialoadenitis). In most cases (91.6 %%) parotid salivary glands were affected. Control group was 15 boys-recruitees - healthy men. On patients hospitalization in rheumatologic department we used conventional diagnostic methods, which included a full clinical and laboratory examination, biopsy and salivary glands puncture followed by morphological examination. According to the goal of the research a crystallographic and crystalloscopic examination of pure saliva was conducted after its collection with a Leshle- Krasnogorskyi capsule [11]. To assess a microcrystallization of pure saliva a modifi ed methodology of P.A. Leus was used and its three types were determined [5, 6]: 1st – per fi eld of vision there are big tree-like crystal formations and drops occupying the entire surface; 2nd - prismatic structure in the centre of the drop, as in the fi rst type, but smaller, on the periphery there are irregularly shaped crystals; 3rd - throughout the saliva droplets a large number of irregular crystal structures are seen. For a complete quantitative characteristics of microcrystals we guided by proposed algorithm [4]. The general background of the agent was studied at low magnifi cation (h40) and microcrystals -was studied at an average magnifi cation (X100) [1]. A detailed description of crystalloscopic presentation was studied at high magnifi cation (h400) according to the following criteria: continuity of main body (Fig. 1), the connection of the fi rst order sprout with the main body, the nature of branches tops - sharp or rounded (Fig. 2), the width of the main body. Besides the description of microcrystals, organic inclusions were also evaluated (Fig. 3): the ratio of their area to the fi eld of view, location (on the periphery, in the centre or around the fi eld); position towards a crystal (sticking or insulation). Fig. 1. Continuity of main body Fig. 2. The nature of branches tops - sharp (left) and rounded (right) А В Fig. 3. Organic inclusions: single (A) and multiple (B). Results and discussion. According to the conclusion of histological laboratory all tumour in patients were represented by B-cell MALT lymphomas. Data analysis of crystalloscopic picture (table) has shown that in individuals with apparently healthy oral cavity there was formed at least 1-2 kinds of crystals and 13-15 variants of dendritic crystals in saliva, 6 signs of dendritic crystals were always present in crystallogram. In 60% of patients with Sjogren disease the 3rd type of crystallogram was noted, and 40% has the 2nd type. This indicates on a violation of crystallization properties of saliva in the development of tumours in the salivary glands. In malignant tumours the 3rd type microcrystallization was determined in 100% of patients. They had the following crystallographic picture of pure saliva: the location of crystallization centres on the surface of a Petri dish were mostly uniform, the number of crystallization centres ranged from 8 to 12 and averaged 10. Rays collected in the form of bundles, the number of which reached 8, proceeded from the centre of crystallization. Kinks and sharp thickening of crystals rays were observed. They become less transparent, in the central part their colour was dark gray, and clearing was closer to the periphery. Rays were deformed by numerous constrictions, interception, which led to their separation and consolidation. Index of average number of crystallization centres is higher in patients compared to crystallograms of healthy persons. In patients with tumours, on the matted crystallization surface a short needle deformed shape crystals were found. They had a tendency to crystallization centres, whose number ranged from 10 to 20. In the centre of crystallization there was a dark-gray mass, in which, even with a signifi cant increase, it was not possible to fi nd the form of small and large pieces of crystals. This peculiar arrangement of crystals resembled a dark-gray “cruciform” shapes. In the fi eld of crystallization they were located at a considerable distance. Tab. 1. Crystalloscopic picture of saliva in healthy persons (P.A. Leus criteria) and patients with Sjogren disease and lymphoma Structures Healthy persons Sjogren disease Lymphoma Monocrystalline fi gures Rectangles 4 2 1 Prisms 2 0-1 0 Pyramids 1 0 1 Octahedron 0 1 0 Polycrystalline structures Linear 1 2 0-1 Rectangles 0-1 1 0-1 “Moss” 2-3 0 0 “Cross” 0-1 0 0 “Horsetai” 0 6 5-7 “Rosette” 0 0 0 Amorphous bodies Size Medium Medium Small Quantity Medium Large Medium Conclusions. Thus, when comparing saliva crystallographic pictures of patients with lymphoma and Sjogren disease, the most characteristic for the fi rst one is the formation of a large number of short and wide needle-shape crystals and the presence of socalled cruciform shapes. By most criteria in these patients the distinct crystallographic differences of saliva, compared with healthy individuals, are observed. Such specifi c criteria enable to insist on features of crystallographic characteristics of saliva in lymphoproliferative diseases of salivary glands. The use of crystallography and crystalloscopy of saliva on the early stages of the examination of patients with suspected B-lymphoma of salivary gland enables its early diagnosis and differentiation. High research precision, noninvasiveness of this atraumatic method makes possible its wide application in daily work of practicing physicians in outpatient and hospital facilities.Документ Changes in nitric oxide production and development of oxidative stress in rats heart during prolonged triptorelin-induced central deprivation of luteinizing hormone synthesis(Poltava State Medical University, Poltava, 2022) Voroshilova, T. A.; Shepitko, V. I.; Stetsuk, Ye. V.; Akimov, O. Ye.; Puzyryov, G. S.; Ворошилова, Тетяна Анатоліївна; Шепітько, Володимир Іванович; Стецук, Євген Валерійович; Акімов, Олег Євгенович; Пузирьов, Гай СергійовичCentral deprivation of luteinizing hormone synthesis for 365 days leads to the development of oxidative stress in rat hearts. The peak of oxidative damage to the heart under these conditions happens on the 180th day of the central deprivation of luteinizing hormone synthesis. Production of nitric oxide during central deprivation of luteinizing hormone synthesis undergoes complex changes from an initial decrease of NO-synthase-dependent nitric oxide production on the 30th and 90th day to overproduction of nitric oxide from NO-synthases on the 365th day of the experiment.Документ Changes in the circadian rhythm of metabolic rates in the saliva of patients with compensated type 2 diabetes mellitus(2019) Gozhenko, А. I.; Hryshko, Yu. M.; Gorbach, T. V.; Гоженко, Анатолій Іванович; Гришко, Юлія Михайлівна; Горбач, Т. В.Even in compensated type 2 DM, thereare changes in the energy metabolism, induced by hormonal regulation. These changes can be controlled using the daily saliva monitoring.Документ Circadian rhythm of metabolic rates in the saliva of patients with arterial hypertension against the background of type 2 diabetes mellitus(2019) Gozhenko, A. I.; Hryshko, Yu. M.; Gorbach, T. V.; Гоженко, Анатолій Іванович; Гришко, Юлія Михайлівна; Горбач, Т. В.The study of the daily rhythm of in the saliva parameters makes it possible to conclude that in DM2, and especially in its progress, as evidenced by the accession of hypertension, there are disorders of FMC. One of its consequences is the development of oxidative stress, which is considered as one of the leading secondary mechanisms in the pathogenesis of DM2.Документ Circadian rhythm of metabolism indicators in healthy people according to saliva study findings(2018) Hryshko, Yu. M.; Gorbatch, T. V.; Gozhenko, A. I.; Гришко, Юлія Михайлівна; Горбач, Т. В.; Гоженко, Анатолій ІвановичThe obtained results, firstly, represent the existence of daily changes in FMC, and secondly, constitute a possible information channel that can be used to study these changes, to determine and compare indicators in the morning and evening about the functional activity of the body involving the regulatory systems.Документ Dynamics of CD68 receptor expression in macrophages of the interstitial space of the rat testis under triptorelin prolonged administration(Ukr. Biochem. J., 2023) Stetsuk, Ye. V.; Shepytko, V. I.; Akimov, O. Ye.; Boruta, N. V.; Rud, M. V.; Pelypenko, L. B.; Lysachenko, O. D.; Vilkhova, O. V.; Skotarenko, T. A.; Voloshyna, O. V.; Стецук, Євген Валерійович; Шепітько, Володимир Іванович; Акімов, Олег Євгенович; Борута, Наталія Володимирівна; Рудь, Марія Володимирівна; Пелипенко, Лариса Борисівна; Лисаченко, Ольга Дмитрівна; Вільхова, Олена Вікторівна; Скотаренко, Тетяна АнатоліївнаTestosterone, the production of which is stimulated by the release of luteinizing hormone (LH) has a remarkable anti-inflammatory and immunomodulatory effect, and in conditions of testosterone deficiency tissue damage can occur due to excessive differentiation of macrophages into a proinflammatory M1 phenotype. The aim of this study was to determine the spread of CD68 receptors as the marker of inflammation on the cells in the interstitial space and testicular vessels under LH synthesis blockade with tryptorelin. Sexually mature white male rats were randomly divided into control (10 animals) and experimental (25 animals) groups. Animals in the experimental group were injected with a tryptorelin acetate solution (0.3 mg/kg). Immunochemical analysis of CD68+ expression was estimated at Olympus FV10i-LIV laser scanning confocal microscope using fluorescent labeling dye. It was shown that luteinizing hormone deprivation led to an increase in the distribution of the CD68 receptor in the interstitial space and in the testicular vessels from day 30 to 180 of the experiment, associated with the increase of inducible NO synthase activity in testis tissue.Документ Effect of combined nitrate-fluoride intoxication on the function of NO-synthases and arginases in the gastric mucosa, blood and heart of rats(JSciMed Central®, 2016) Akimov, O. Ye.; Kostenko, V. O.; Костенко, Віталій Олександрович; Акімов, Олег ЄвгеновичThe focus of this article is on the functioning of NO-synthases and arginases in the gastric mucosa, blood and heart of white rats under excessive intake of sodium fluoride and sodium nitrate during 30 days. Excessive intake of sodium nitrate was modeled by infusion of aqueous solution into stomach through special catheter at a dose of 500 mg / kg. Excessive intake of sodium fluoride was modeled by infusion of aqueous solution into stomach through special catheter at a dose of 10 mg / kg. The activity of these enzymes was measured by spectrophotometric methods. Analysis of the influence produced by combined nitrate-fluoride intoxication on total NOS activity in the gastric mucosa, heart and blood showed the presence of antagonistic effect of nitrates and fluorides. Nitrates act as NOS inhibitors, whereas the fluorides are inducers of NOS. Under their combined action in the organism, fluoride-induced elevation prevails over nitrate-induced inhibition, but only in tissue homogenates. Such effect is absent in blood. The inductive effect of combined nitrate-fluoride intoxication on functioning of arginases is mediated by nitrate release of L-arginin from NOS. Combined intoxication showed prevalence of inducing influence of nitrates over inhibiting effect of fluoridesДокумент Energy-saving and environmentally-friendly methods extraction of tungsten and tungsten ore concentrate – an integral part of environment and human healt(2014) Chernenko, L. A.; Solovieva, N. V.; Черненко, Людмила Олексіївна; Соловйова, Наталія ВеніамінівнаIt is shown that tungsten is extracted from tungsten ores and concentrates in sodium chloride-sodium metasilicate melts into the halide phase at a temperature of 1050 to 1050-1100°C. The compositions of the melts that provide the extraction efficiency as high as 99% and maximal ratios of separation of the components between the phases are chosen.Документ Extracellular matrix of rat liver under the conditions of combining systemic inflammatory response syndrome and chronic alcohol intoxication(Полтавський державний медичний університет, 2022) Mykytenko, A. O.; Akimov, O. Ye.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне СтепанівнаThe aim of this study was to establish the effect of systemic inflammatory response syndrome on the background of chronic alcohol intoxication on the concentration of oxyproline, glycosaminoglycans and sialic acids in the liver of rats. The study was performed on 24 male Wistar rats. Animals were divided into 4 groups of 6 animals: I – control; II – systemic inflammatory response syndrome group; III – alcoholic hepatitis groupand IV – animals with a combination of systemic inflammatory response syndrome and chronic alcohol intoxication. The concentrations of glycosaminoglycans, heparin-heparan, keratan-dermatan and chondroitin fractions of glycosaminoglycans, free oxyproline and sialic acids were studied in the liver tissue homogenate. The combination of systemic inflammatory response syndromeand chronic alcohol intoxication leads to ncreased intensity of collagenolysis, glycoprotein catabolism and reduced intensity of breakdown of proteoglycans of connective tissue of the liver. However, it changes the ratio of individual fractions of glycosaminoglycans in the direction of reducing тtheconcentration of anti-inflammatory and increasing regenerative.Документ Influence of carboline on reactive oxygen and nitrogen species production in gastric mucosa of rats under chronic nitrate-fluoride intoxication(Національний фармацевтичний університет, 2018-10-18) Akimov, O. Ye.; Акімов, Олег ЄвгеновичCertain regions of Ukraine have risk of simultaneous intake of excessive concentrations of nitrates and fluorides with drinking water and food. Fluorides can enter groundwater since they are byproduct of aluminum production. Some regions of Ukraine have high concentration of fluorides in groundwater due to high concentration of fluorine containing salts in ground. Poltava region can serve as good example of such regions. Nitrates are used as cheap and effective fertilizers. Therefore vegetables are the primary source of excessive nitrates intake. Gastro-intestinal system is the first target for adverse effects of both mentioned above environmental pollutants during their alimentary consumption. Combined effect of nitrates and fluorides on gastro-intestinal system is not well researched. The aim of this study is to determine influence of “Carboline” on production of superoxide anion radical (O2˙-) and peroxynitrite (ONOO-) in rats gastric mucosa under chronic nitrate-fluoride intoxication. Materials and methods. Experiment was performed on 38 Wistar rats weighting 180-230 g. Animals were divided into 3 groups: control group (10), combined intoxication group (15) and “Carboline” group (13). Chronic nitrate-fluoride intoxication (combined intoxication) was induced as described in our previous work [1]. Animals of “Carboline” group were receiving suspension of “Carboline” at a dose 100 mg/kg during combined intoxication induction. Animals were sacrificed under thiopental anesthesia by bloodletting. All biochemical studies were performed in 10% tissue homogenate. Production of O2˙- was determined as described in [2]. Production of ONOO- was determined by increase in concentration of peroxynitrites of alkali and alkali-base metals after 40 min incubation in buffer solution (ph=7.4). Concentration of peroxynitrites of alkali and alkali-base metals was determined as described in [1]. Results were analyzed by Mann-Whitney method. Differences between groups were deemed statistically significant if p<0.05. Results and discussion. Combined intoxication increases production of O2˙- by 195% compared to control group. Production of ONOO- elevates by 17.64%. Suspension of “Carboline” decreases O2˙- production by 21% compared to combined intoxication group. Production of ONOO- drops by 25%. Increased O2˙- production during combined intoxication owns to the fact that both nitrates and fluorides can influence mitochondrial electron transport chain. There are evidences in literature that fluoride ions can cause tissue hypoxia by impairing mitochondrial electron transport. However exact mechanism is not known yet. Cytochromes of mitochondria have ability to reduce nitrates to nitrites sacrificing two electrons in the process. Under physiologic conditions electrons from cytochromes should be used in ATP synthesis. Two electron reduction of nitrates to nitrites in eukariotic cells does not lead to ATP synthesis. This “electron leakage” creates possibility of uncoupling of oxidative phosphorylation and ATP synthesis thus leading to O2˙- production by mitochondria. Judging from almost twofold increase in O2˙- production during combined intoxication we can assume that nitrates and fluorides have synergetic effect on O2˙- production. ONOO- is formed in reaction of O2˙- with nitric oxide (NO). Both nitrates and fluorides can lead to increased production of NO [1]. However sources of increased production of NO are different for fluorides and nitrates. Fluorides can increase NO production by activation of inducible NO-synthase, while nitrates can provide excessive amounts of NO by activation of nitrate reductases. “Carboline” is a sorbent which consists from carbon fibers obtained by extrusion of crushed tissue carbon fiber sorbent of AUT –M brand. “Carboline” has sorption surface of 2500 sq. meters per 1 gram, which allows it efficiently absorb nitrates and fluorides from gastro-intestinal tract. Conclusion. Suspension of “Carboline” is effective for correction of increased reactive oxygen and nitrogen species production in gastric mucosa of rats under nitrate-fluoride intoxication.Документ Influence of chronic nitrate intoxication on the concentration of lipid hydroperoxides in the gastric mucosa(Українська медична стоматологічна академія, 2018-10) Akimov, O. Y.; Акімов, Олег ЄвгеновичThe excessive alimentary intake of nitrates into human and animal body can cause a cascade of changes in their organism. The sources of nitrates can be either products of vegetable origin (especially beetroot) or groundwater and drinking water (regions with developed agriculture). The first system of organs, which experiences the negative effect of nitrates, is the digestive system. The purpose of this work was to establish the effect of chronic nitrate intoxication on the content of lipid hydroperoxides in rat stomach mucous membrane. Materials and methods. We carried out experiment on 24 white rats. We divided animals into 2 groups: the control group (10) and the group of chronic nitrate intoxication (14). We modeled chronic nitrate intoxication by intragastric administration of sodium nitrate at a dose of 500 mg / kg for 30 days. The content of lipid hydroperoxides in 10% of the mucous membrane homogenate was determined by the method of VB Gavrilov. and MI Mishkorudnaya (1983). We analyzed the obtained results for normality by the Shapiro-Wilk method. Further, we compared data by the Student method. The difference was considered statistically significant if p <0.05. Results and discussion. Chronic nitrate intoxication reduces the content of dien conjugates (DC) in the gastric mucosa by 8% relative to the control group. The concentration of triene conjugates (TC) increases by 20.51%. The content of octadiene conjugates (ODC) increases by 52.2%. Decrease in the concentration of DC under conditions of nitrate intoxication, we can explain by the increase in activity of superoxide dismutase [1]. The remaining hydroperoxides are products of the later stages of lipid peroxidation (LPO), which is controlled by the glutathione system. Thus, it can be argued that the excessive intake of nitrates in the body leads to a disruption in the functioning of the glutathione system. Conclusion. Excess intake of nitrates in the body prevents the activation of LPO on early stages, but enhances lipid oxidation that has already begun.Документ Influence of combined nitrate and fluoride intoxication on connective tissue disorders in rats gastric mucosa(2019) Akimov, O. Y.; Mischenko, A. V.; Kostenko, V. O.; Акімов, Олег Євгенович; Міщенко, Артур Володимирович; Костенко, Віталій ОлександровичWe established that chronic excessive fluoride or nitrate intake can cause oxidative and nitrosative stress-depended collagen degradation, simultaneously decreasing the concentration of anti-inflammatory GAG. Combined intoxication leads to even more severe connective tissue degradation, but subsequently activates compensatory mechanisms through increase in concentration of anti-inflammatory GAG.Документ Influence of inhibitors of transcription factor kappa B on depolimerization of biopolymers in periodontal connective tissue under systemic intlammatory response in rats(Українська медична стоматологічна академія, 2020) Yelinska, A. M.; Denisenko, S. V.; Liashenko, L. I.; Kostenko, V. O.; Єлінська, Аліна Миколаївна; Денисенко, Софія Валеріївна; Ляшенко, Лілія Іванівна; Костенко, Віталій Олександрович; Елинская, Алина Николаевна; Денисенко, София Валерьевна; Ляшенко, Лилия Ивановна; Костенко, Виталий АлександровичThe use of ammonium pyrrolidine dithiocarbamate (APDTC), an inhibitor of the nuclear translocation of the transcription factor κB (NF-κB), under the conditions of systemic administration of Salmonella typhi lipopolysaccharide is an effective means in correction of periodontium connective tissue disorganization of the periodontium in rats, reduces depolymerization of collagen, proteoglicans and sialoglycoproteins in soft and osseous tissues of periodontium, as well as decreases alveolar process resorption.Документ Influence of nanosized cerium dioxide on the vascular bed of the liver under the conditions of chronic alcoholic hepatitis modeling(Полтавський державний медичний університет, 2024) Mykytenko, A. O.; Akimov, O. Y.; Yeroshenko, G. A.; Neporada, K. S.; Микитенко, Андрій Олегович; Акімов, Олег Євгенович; Єрошенко, Галина Анатоліївна; Непорада, Каріне СтепанівнаChronic alcohol consumption changes the microcirculation of the liver and, depending on the duration and dose of ethanol, can have various negative consequences. Nanosized cerium dioxide can be an effective means of pathogenetic correction of ethanol-induced changes in the liver. The aim of the work was to study the effect of nanosized cerium dioxide on the morphometric indicators of the vascular bed of rat liver under the conditions of chronic alcoholic hepatitis modeling. The experiments were performed on 24 white, mature male Wistar rats, weighing 180–220 g. Animals modeled chronic alcoholic hepatitis according to Stepanov Yu.M. (2017) and nanosized cerium dioxide was administered intragastrically at a dose of 1 mg/kg in a volume of 2.9 ml/kg. The morphometric parameters of the inner diameter of the vessels of the liver lobe were determined; capillary lumen around the central vein and hepatic triad. Simulation of chronic alcoholic hepatitis leads to expansion of the central vein and narrowing of interlobular arteries and veins and lobular arterioles and venules. The introduction of nanodispersed cerium dioxide against the background of modeling chronic alcoholic hepatitis expands alcohol-narrowed lumens of interlobular arteries and veins and lobular arterioles and venules, without affecting the diameter of the central vein. Хронічне вживання алкоголю змінює мікроциркуляцію печінки і, в залежності від тривалості і дози етанолу, може мати різні негативні наслідки. Нанодисперсний діоксид церію може бути ефективним засобом патогенетичної корекції етанол-індукованих змін в печінці. Метою роботи було вивчити вплив нанодисперсного діоксиду церію на морфометричні показники мікроциркуляторного русла печінки щурів за умов моделювання хронічного алкогольного гепатиту. Експерименти виконані на 24 білих статевозрілих щурах-самцях лінії Wistar, вагою 180-220 г. Тварини моделювали хронічний алкогольний гепатит за Степановим Ю.М. (2017) і внутрішньошлунково вводили нанокристалічний діоксид церію в дозі 1 мг/кг об’ємом 2,9 мл/кг. Визначали морфометричні параметри внутрішнього діаметра судинного русла печінкової часточки; просвіт капілярів навколо центральної вени та печінкової тріади. Моделювання хронічного алкогольного гепатиту призводить до розширення центральної вени та звуження міжчасточкових артерій та вен і часточкових артеріол та венул. Введення нанодисперсного діоксиду церію на фоні моделювання хронічного алкогольного гепатиту розширює звужені алкоголем просвіти міжчасточкових артерій та вен і часточкових артеріол та венул, не впливаючи на діаметр центральної вени.Документ Lipid peroxidation and antioxidant protection in periodontaltissues under the action of local pathogenic factor on gums in rats exposed to modeled systemic inflammatory response(Українська медична стоматологічна академія, 2017) Yelins’ka, A. M.; Kostenko, V. O.; Єлінська, Аліна Миколаївна; Костенко, Віталій ОлександровичThe purpose of the work was to study the state of lipid peroxidation (LPO) and antioxidant system in periodontal tissues under the influence of the local pathogenic factor on the gums in rats against modeled systemic inflammatory response (SIR)reproduction. The study was carried out on 40 white male rats of the Wistar line weighing 180-220 g, divided into 4 groups: the 1stincluded intact animals, the 2nd was made up by animals subjected to SIR modeling, the 3rdincluded animals, which were subjected to modeled acute gingivitis by applying 5% sodium hydroxide (NaOH) solution onto their gums, and the 4thgroup involved rats with acute gingivitis induced by 5% NaOH solution against modeled SIR. SIR was induced by intraperitoneal administration of lipopolysaccharide Salmonella typhi (pyrogenalum) in a dose that stimulated rise in temperature by 1.5 °C. During the following seven weeks of the experiment, rats were given 4 MPD / kg of body weight once a week. To simulate the action of local pathogenic factor affecting the gums we used the modeled acute gingivitis. For this purpose we irrigated the rats’ gums with 5% NaOH solution through 10 s. The simulation of SIRis ac-companied by the development of decompensated LPO in periodontal tissues, by the decrease in their antioxidant poten-tial as well as the activity of superoxide dismutase and catalase. The application of 5% NaOH solution onto thegums is accompanied by the development of compensated LPO in periodontal tissues with an increase in the activity of superox-ide dismutase and catalase. The application of 5% NaOH solution onto the gums against modeled SIR causes an in-crease in the production of by-products of LPO in periodontal tissues and reduced their antioxidant potential, as well as the activity of superoxide dismutase and catalase compared to separate simulations of SIR and acute gingivitis.Документ Methodical approach to teaching students mechanisms of hemoblastosis development at the department of pathophysiology(Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2, 2021-01) Yelinska, A. M.; Єлінська, Аліна МиколаївнаThis work is a fragment of the initiative research "The role of transcription factors, the circadian oscillator system and metabolic disorders in the formation and functioning of pathological systems" (№ 0119U103898). The article is devoted to the problem of methodical approach to teaching the topic "Hemoblastosis" at the Department of Pathophysiology. Module 2 "Pathophysiology of organs and systems" begins with the study of the submodule "Pathophysiology of the blood system" for all students majoring in 222 "Medicine", 228 "Pediatrics" and 221 "Dentistry". One of the difficult topics for students to understand is the topic of "Hemoblastosis". In Ukraine, the system of higher medical education is undergoing active innovative changes. This process is characterized by the introduction of new technologies and techniques in the educational process. Innovative forms and methods of teaching are developed and implemented. The purpose of this work is to analyze the difficulties that students have when they studying the topic of "Hemoblastosis" and the development of methodological approaches to their elimination. According to the WHO: cancer is one of the leading causes of death in the world: in 2012, there were 8.2 million deaths from cancer. Hemoblastosis accounts for approximately 8% of all malignancies and all together they are among the 6 most common types of malignant tumors. Acute leukemias account for about 50-60% of all leukemias, with acute myeloblastic leukemia being slightly more common than acute lymphoblastic leukemia. Chronic leukemias account for about 40-50% of all leukemias, with chronic lymphocytic leukemia being slightly more common than chronic myelocytic leukemia. Hemoblastosis is a relatively rare disease; mortality from them is 1.7-3.1 per 100,000 population. Among therapeutic diseases, leukemia occurs in 1.5-2.6% of cases. Recently, the incidences of hemoblastosis, especially acute, have increased in all countries.Документ Modeling the electronic structure and composition tungsten adsorbed metal complexes of on the electrode surface under conditions of high-temperature electrochemical synthesis(Science Publishing Corporation, 2018) Solyanik, Liudmyla; Kuznetsova, Tetiana; Solovyova, Nataliia; Usenko, Dmytro; Соловйова, Наталія ВеніамінівнаThe interest to the physics of nano-dimensional structures, the most urgent and most dynamically developing field of modern solid state physics, is connected both with completely new fundamental scientific problems and physical phenomena, and with the prospects of creating entirely new quantum devices and systems with wide functional opportunities for nano-electronics, measuring equipment, new- generation information technologies, communication equipment, etc.Документ NF-κB-опосередкований вплив NO-синтаз на вільнорадикальні процеси у тканинах пародонта за умов експериментального метаболічного синдрому(Вищий державний навчальний заклад України "Українська медична стоматологічна академія", 2014) Ляшенко, Лілія Іванівна; Ляшенко, Лилия Ивановна; Lyashenko, L. I.; Костенко, Виталий Александрович; Костенко, Віталій Олександрович; Kostenko, V. O.У експерименті на 40 білих щурах досліджено роль NF-κB у механізмах порушень вільнорадикальних процесів, залежних від функціонального стану NOS, у тканинах пародонта при моделюванні метаболічного синдрому (МС). Показано, що здатність нейрональної NO-синтази (nNOS) регулювати (обмежувати) пероксидне окиснення ліпідів (ПОЛ) у м’яких тканинах пародонта за умов МС є NF-κB-опосередкованим. Збільшення при введенні селективного інгібітора nNOS утворення ТБК-активних сполук та зниження антиоксидантного (АО) потенціалу усувається призначенням інгібітора ядерної транслокації NF-κB JSH-23 (4-метил-N-(3-фенілпропіл)бензол-1,2-діаміну). Показано, що дія селективного інгібітора індуцибельної NO-синтази аміногуанідину за умов МС не супроводжується NF-κB-залежними змінами ПОЛ і АО потенціалу в м’яких тканинах пародонта. Введення JSH-23 за умов експерименту супроводжується підвищенням АО дії L-аргініну; В эксперименте на 40 белых крысах исследована роль NF-κB в механизмах нарушений свободнорадикальных процессов, зависящих от функционального состояния NOS, в тканях пародонта при моделировании метаболического синдрома (МС). Показано, что способность нейрональной NO-синтазы (nNOS) регулировать (ограничивать) пероксидное окисление липидов (ПОЛ) в мягких тканях пародонта при МС является NF-κB-опосредованным. Увеличение при введении селективного ингибитора nNOS образования ТБК-активных соединений и снижение антиоксидантного (АО) потенциала устраняется назначением ингибитора ядерной транслокации NF-κB JSH-23 (4-метил-N- (3-фенилпропил) бензол- 1,2-диамина). Показано, что действие селективного ингибитора индуцибельной NO-синтазы аминогуанидина в условиях МС не сопровождается NF-κB-зависимыми изменениями ПОЛ и АО потенциала в мягких тканях пародонта. Введение JSH-23 в условиях эксперимента сопровождается повышением АО действия L-аргинина; The role of NF-κB in the mechanisms of free radical processes impairment depending on the functional state of NOS in periodontal tissues under modeled metabolic syndrome (MS) was studied in the experiment on 40 white rats. We have shown the ability of neuronal NO-synthases (nNOS) to regulate (limit) lipid peroxidation (LPO) in periodontal soft tissues under conditions of MS is NF-κB- mediated. The introduction of selective nNOS inhibitor leads to the increase in the formation of TBA- active compounds, while the reduction in antioxidant potential (AO) is eliminated by the inhibitor of nuclear translocation of NF-κB JSH- 23 (4-methyl-N- (3-phenylpropyl) benzene -1,2-diamine). It has been shown that the effect of selective inhibitor of inducible NO-synthase aminoguanidine under MS is not accompanied by NF-κBdependent changes of LPO and AO capacity in periodontal tissues. The introduction of JSH-23 under conditions of metabolic syndrome is accompanied by increased AO action of L-arginineДокумент NO- и пероксинитрит-зависимые изменения продукции супероксидного анион-радикала в органах крыс при экспериментальном метаболическом синдроме(Гродненский государственный медицинский университет, 2014) Костенко, Віталій Олександрович; Єлінська, Аліна Миколаївна; Ляшенко, Лілія Іванівна; Соловйова, Наталія Веніамінівна; Талаш, Вікторія Володимирівна; Костенко, Виталий Александрович; Елинская, Алина Николаевна; Ляшенко, Лилия Ивановна; Соловьева, Наталья Вениаминовна; Талаш, Виктория Владимировна; Kostenko, V. O.; Yelinskaya, A. M.; Lyashenko, L. I.; Solovyeva, N. V.; Talash, V. V.В эксперименте на 30 белых крысах-самцах линии Вистар массой 180-230 г исследована роль различных изоформ NO-синтазы и пероксинитрита в продукции супероксидного анион-радикала (.О ) в различных органах (пародонте, поднижнечелюстных слюнных железах, аорте, семенниках) в условиях воспроизведения углеводно-жировой модели метаболического синдрома (МС). Образование. О оценивали спектрофотометрически при проведении теста с нитросиним тетразолием в гомогенате тканей с индукторами в виде НАДН и НАДФН для оценки продукции О, соответственно, митохондриальной и микросомальной электронно-транспортными цепями (ЭТЦ). Выявили, что воспроизведение МС сопровождается усилением продукции .О указанными ЭТЦ в тканях пародонта, СЖ,аорты и семенников. Функциональная активность нейрональной NO-синтазы (nNOS) при экспериментальном МС обеспечивает ограничение продукции .О митохондриальной и микросомальной ЭТЦ. Введение селективного ингибитора nNOS 7-нитроиндазола повышает выработку .О этими источниками в тканях всех исследуемых органов. Функциональная активность индуцибельной NO-синтазы (iNOS) при моделировании МС способствует генерации .О . Введение селективного ингибитора iNOS аминогуанидина снижает продукцию .О митохондриями (в тканях пародонта, слюнных желез, аорты и семенников) и микросомами (в слюнных железах). Применение скэвенджера пероксинитрита L-селенометионина при моделировании МС ограничивает гиперпродукцию .О митохондриальной ЭТЦ (в тканях пародонта и слюнных желез), однако не влияет на генерацию .О НАДФН-оксидазой микросом.Документ NO- та NF-κB –залежні механізми порушення білоксинтезуючої функції слинних залоз щурів за умов експериментального метаболічного синдрому(Вищий державний навчальний заклад України «Українська медична стоматологічна академія», 2014) Єлінська, Аліна Миколаївна; Костенко, Віталій Олександрович; Елинская, Алина Николаевна; Костенко, Виталий Александрович; Yelinska, A.; Kostenko, V. O.У експерименті на 40 білих щурах досліджено роль NO-синтаз і ядерного фактора κB (NF-κB) у механізмах порушень білоксинтезуючої функції піднижньощелепних слинних залоз (СЗ) за умов моделювання метаболічного синдрому (МС). Показано, що ця функція СЗ залежить від функціонального стану NO-синтаз. Функціонування нейрональної NO-синтази за цих умов сприяє збільшенню активності α-амілази у тканинах СЗ. Функціонування індуцибельної NO-синтази за умов експерименту пригнічує активність α-амілази та орнітиндекарбоксилази у СЗ. Призначення L-аргініну під час відтворення МС суттєво не впливає на активність α-амілази у тканинах СЗ, але обмежує зниження активності орнітиндекарбоксилази. Введення скевенджеру пероксинітриту L-селенометіоніну та інгібітора активації NF-κB JSH-23 за умов експериментального МС істотно покращує білоксинтезуючу функцію СЗ, що не є характерним при призначенні метформіну гідрохлориду; В эксперименте на 40 белых крысах исследована роль NO-синтаз и ядерного фактора κB (NF-κB) в механизмах нарушений белоксинтезирующей функции поднижнечелюстных слюнных желез (СЖ) в условиях моделирования метаболического синдрома (МС). Показано, что данная функция СЖ зависит от функциональногосостояния NO-синтаз. Функционирование нейрональной NO-синтазы в этих условиях способствует увеличению активности α-амилазы в тканях поднижнечелюстных СЖ. Функционирование индуцибельной NO-синтазы в условиях эксперимента угнетает активность α-амилазы и орнитиндекарбоксилазы (ОДК) в СЖ. Назначение L-аргинина существенно не влияет на активность α- милазы в тканях СЖ, но ограничивает снижение активности ОДК. Введение скэвенджера пероксинитрита L-селенометионина и ингибитора активации NF-κB JSH-23 в условиях экспериментального МС существенно улучшает белоксинтезирующую функцию СЖ, что не характерно при назначении метформина гидрохлорида; The role of NO-synthases and nuclear factor κB (NF-κB) in the mechanisms impairing protein synthesis function of submandibular salivary glands (SSG) under modeled metabolic syndrome (MS) was investigated on 40 white rats. We have found out this function depends on the functional state of NO-synthases. Functioning of neuronal NO-synthase under these conditions increases the activity of α-amylase in SSG tissues. Functioning of inducible NO-synthase in experimental conditions inhibits the activity of α-amylase and ornithine decarboxylase in SSG. Administration of L-arginine during MS modeling did not significantly affect α-amylase activity in the SSG tissues, but limited the decrease in ornithine decarboxylase activity. The introduction of peroxynitrite scavenger L-selenomethionine and NF-κB activation inhibitor JSH-23 under the experimental MS significantly improves protein synthesis in SSG, which is not typical in metformin hydrochloride administration